##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AflII AgeI AhdI AscI
AseI AsnI AspEI BamHI BbrPI
BcgI BciVI BfrI BglII BsiWI
BsmI Bsp1407I BspDI BspHI BsrGI
BssHII BstBI ClaI CpoI DrdI
EagI Eam1105I EclXI Eco52I EcoRV
EcoT22I FseI HpaI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RcaI RsrII SalI SapI SbfI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 5240 GGTACC G^GTACC
AccI 4389 GTATAC GT^[AC][GT]AC
AclI 4563 AACGTT AA^CGTT
Asp718I 5240 GGTACC G^GTACC
AvrII 2713 CCTAGG C^CTAGG
BbeI 114 GGCGCC GGCGC^C
BbvCI 5435 CCTCAGC CCTCAGC(-5/-2)
BclI 2842 TGATCA T^GATCA
BlnI 2713 CCTAGG C^CTAGG
BlpI 974 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 974 GCTCAGC GC^T[ACGT]AGC
BsaAI 376 TACGTG [CT]AC^GT[GA]
BsgI 394 GTGCAG GTGCAG(16/14)
BspLU11I 4417 ACATGT A^CATGT
Bst1107I 4389 GTATAC GTA^TAC
BstZ17I 4389 GTATAC GTA^TAC
CelII 974 GCTCAGC GC^T[ACGT]AGC
DraIII 4245 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 3298 GAATTC G^AATTC
FbaI 2842 TGATCA T^GATCA
HincII 4745 GTTGAC GT[CT]^[GA]AC
HindII 4745 GTTGAC GT[CT]^[GA]AC
KasI 114 GGCGCC G^GCGCC
KpnI 5240 GGTACC GGTAC^C
NarI 114 GGCGCC GG^CGCC
Psp1406I 4563 AACGTT AA^CGTT
ScaI 2045 AGTACT AGT^ACT
SfoI 114 GGCGCC GGC^GCC
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AccIII 170 TCCGGA T^CCGGA
1628 TCCGGA
AcyI 114 GGCGCC G[GA]^CG[CT]C
2028 GACGCC
AflIII 2569 ACGTGT A^C[GA][CT]GT
4417 ACATGT
Alw44I 2128 GTGCAC G^TGCAC
5055 GTGCAC
ApaI 2120 GGGCCC GGGCC^C
5277 GGGCCC
ApaLI 2128 GTGCAC G^TGCAC
5055 GTGCAC
Asp700I 837 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1656 GAAGGACTTC
AspI 444 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1971 GACCCGGTC
AviII 1526 TGCGCA TGC^GCA
1748 TGCGCA
BbsI 1317 GTCTTC GAAGAC(2/6)
1735 GTCTTC
BpuAI 1317 GTCTTC GAAGAC(2/6)
1735 GTCTTC
BsaBI 1410 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4861 GATGTTGATC
BsaHI 114 GGCGCC G[GA]^CG[CT]C
2028 GACGCC
BsaWI 170 TCCGGA [AT]^CCGG[AT]
1628 TCCGGA
BseAI 170 TCCGGA T^CCGGA
1628 TCCGGA
BsmBI 1372 GAGACG CGTCTC(1/5)
2509 GAGACG
BspEI 170 TCCGGA T^CCGGA
1628 TCCGGA
BsrDI 4804 CATTGC GCAATG(2/0)
1091 GCAATG
BssSI 1780 CTCGTG CACGAG(-5/-1)
3638 CTCGTG
BstAPI 396 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
687 GCAGCCCCTGC
BstEII 913 GGTGACC G^GT[ACGT]ACC
3328 GGTGACC
BstPI 913 GGTGACC G^GT[ACGT]ACC
3328 GGTGACC
BstXI 903 CCAACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1466 CCACCTTCATGG
BstYI 1415 AGATCC [GA]^GATC[CT]
2410 GGATCT
DraI 4583 TTTAAA TTT^AAA
5707 TTTAAA
EarI 4132 GAAGAG CTCTTC(1/4)
4840 GAAGAG
EcoO65I 913 GGTGACC G^GT[ACGT]ACC
3328 GGTGACC
FspI 1526 TGCGCA TGC^GCA
1748 TGCGCA
Hin1I 114 GGCGCC G[GA]^CG[CT]C
2028 GACGCC
HindIII 3352 AAGCTT A^AGCTT
4220 AAGCTT
Ksp632I 4132 GAAGAG CTCTTC(1/4)
4840 GAAGAG
MamI 1410 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4861 GATGTTGATC
MflI 1415 AGATCC [GA]^GATC[CT]
2410 GGATCT
MroI 170 TCCGGA T^CCGGA
1628 TCCGGA
NaeI 1880 GCCGGC GCC^GGC
5560 GCCGGC
NgoMIV 1880 GCCGGC G^CCGGC
5560 GCCGGC
PflFI 444 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1971 GACCCGGTC
SexAI 389 ACCAGGT A^CC[AT]GGT
3074 ACCTGGT
SfiI 3084 GGCCCCAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3557 GGCCGGGCAGGCC
Tth111I 444 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1971 GACCCGGTC
XbaI 2904 TCTAGA T^CTAGA
3145 TCTAGA
XhoII 1415 AGATCC [GA]^GATC[CT]
2410 GGATCT
XmnI 837 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1656 GAAGGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI Aor51HI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BglI
BmyI BpmI BsaI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI Eco47III Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
KspI MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PaeR7I PflMI PleI
PpuMI PstI PvuII RsaI SacI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoI