##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI Alw44I ApaLI AscI
Asp718I AvaI AviII AvrII BamHI
BbeI BbrPI BcgI BfrI BglI
BglII BlnI BsaBI BsaWI BseAI
BsiEI BsiWI BsoBI BspDI BspEI
BspHI BsrFI BssHII BssSI BstBI
BstEII BstPI BstUI Cfr10I ClaI
CpoI DrdI EagI EclXI Eco52I
EcoO65I EcoRV FseI FspI KasI
KpnI KspI MamI MluI MroI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SwaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AcyI 160 GGCGTC G[GA]^CG[CT]C
AhdI 3020 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2937 CCTTAGG CC^T[ACGT]AGG
Aor51HI 3687 AGCGCT AGC^GCT
AspEI 3020 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 3392 GAGCAC G[AT]GC[AT]^C
AspI 5024 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 2143 GGTGCC G^G[CT][GA]CC
BciVI 5015 GGATAC GTATCC(6/5)
BclI 405 TGATCA T^GATCA
BcnI 2802 CCGGG CC^[GC]GG
BlpI 1892 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1892 GCTCAGC GC^T[ACGT]AGC
BsaAI 3765 CACGTA [CT]AC^GT[GA]
BsaHI 160 GGCGTC G[GA]^CG[CT]C
BsiHKAI 3392 GAGCAC G[AT]GC[AT]^C
Bsp1407I 922 TGTACA T^GTACA
BsrBI 2998 CCGCTC CCGCTC(-3/-3)
BsrDI 1379 GCAATG GCAATG(2/0)
BsrGI 922 TGTACA T^GTACA
Bst1107I 993 GTATAC GTA^TAC
BstAPI 999 GCAACTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 993 GTATAC GTA^TAC
Bsu36I 2937 CCTTAGG CC^T[ACGT]AGG
CelII 1892 GCTCAGC GC^T[ACGT]AGC
Eam1105I 3020 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3687 AGCGCT AGC^GCT
Eco81I 2937 CCTTAGG CC^T[ACGT]AGG
FbaI 405 TGATCA T^GATCA
Hin1I 160 GGCGTC G[GA]^CG[CT]C
MfeI 5203 CAATTG C^AATTG
MunI 5203 CAATTG C^AATTG
NciI 2802 CCGGG CC^[GC]GG
NdeI 471 CATATG CA^TATG
PflFI 5024 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SpeI 434 ACTAGT A^CTAGT
SspBI 922 TGTACA T^GTACA
Tth111I 5024 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2428 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 277 GTCTAC GT^[AC][GT]AC
993 GTATAC
ApaI 4431 GGGCCC GGGCC^C
4661 GGGCCC
AseI 5501 ATTAAT AT^TAAT
5875 ATTAAT
AsnI 5501 ATTAAT AT^TAAT
5875 ATTAAT
BalI 52 TGGCCA TGG^CCA
1638 TGGCCA
BbvCI 816 CCTCAGC CCTCAGC(-5/-2)
951 CCTCAGC
BsaI 3085 GAGACC GGTCTC(1/5)
1193 GGTCTC
BsmBI 1202 CGTCTC CGTCTC(1/5)
3424 GAGACG
BspLU11I 2820 ACATGT A^CATGT
3264 ACATGT
BstYI 812 AGATCC [GA]^GATC[CT]
2884 GGATCT
DsaI 1797 CCATGG C^C[GA][CT]GG
4427 CCATGG
EaeI 52 TGGCCA [CT]^GGCC[GA]
1638 TGGCCA
EcoNI 241 CCTCCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4189 CCTTAGAGAGG
EcoT22I 1603 ATGCAT ATGCA^T
3900 ATGCAT
FauI 333 CCCGC CCCGC(4/6)
1765 CCCGC
HaeII 3314 GGCGCT [GA]GCGC^[CT]
3687 AGCGCT
HapII 1134 CCGG C^CGG
2802 CCGG
HpaI 3760 GTTAAC GTT^AAC
4467 GTTAAC
HpaII 1134 CCGG C^CGG
2802 CCGG
MflI 812 AGATCC [GA]^GATC[CT]
2884 GGATCT
MluNI 52 TGGCCA TGG^CCA
1638 TGGCCA
MscI 52 TGGCCA TGG^CCA
1638 TGGCCA
MspI 1134 CCGG C^CGG
2802 CCGG
NcoI 1797 CCATGG C^CATGG
4427 CCATGG
NsiI 1603 ATGCAT ATGCA^T
3900 ATGCAT
PshBI 5501 ATTAAT AT^TAAT
5875 ATTAAT
ScaI 1970 AGTACT AGT^ACT
2945 AGTACT
StuI 2910 AGGCCT AGG^CCT
5391 AGGCCT
XhoII 812 AGATCC [GA]^GATC[CT]
2884 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AvaII
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
EarI EcoO109I EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XmnI