##################################
# NO CUT ENZYMES (   92 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AgeI        Alw44I      ApaLI       AscI        
Asp718I     AvaI        AviII       AvrII       BamHI       
BbeI        BbrPI       BcgI        BfrI        BglI        
BglII       BlnI        BsaBI       BsaWI       BseAI       
BsiEI       BsiWI       BsoBI       BspDI       BspEI       
BspHI       BsrFI       BssHII      BssSI       BstBI       
BstEII      BstPI       BstUI       Cfr10I      ClaI        
CpoI        DrdI        EagI        EclXI       Eco52I      
EcoO65I     EcoRV       FseI        FspI        KasI        
KpnI        KspI        MamI        MluI        MroI        
MvnI        NaeI        NarI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SphI        Sse8387I    SwaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AcyI          160  GGCGTC               G[GA]^CG[CT]C
AhdI         3020  GACATTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         2937  CCTTAGG              CC^T[ACGT]AGG
Aor51HI      3687  AGCGCT               AGC^GCT
AspEI        3020  GACATTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        3392  GAGCAC               G[AT]GC[AT]^C
AspI         5024  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI         2143  GGTGCC               G^G[CT][GA]CC
BciVI        5015  GGATAC               GTATCC(6/5)
BclI          405  TGATCA               T^GATCA
BcnI         2802  CCGGG                CC^[GC]GG
BlpI         1892  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1892  GCTCAGC              GC^T[ACGT]AGC
BsaAI        3765  CACGTA               [CT]AC^GT[GA]
BsaHI         160  GGCGTC               G[GA]^CG[CT]C
BsiHKAI      3392  GAGCAC               G[AT]GC[AT]^C
Bsp1407I      922  TGTACA               T^GTACA
BsrBI        2998  CCGCTC               CCGCTC(-3/-3)
BsrDI        1379  GCAATG               GCAATG(2/0)
BsrGI         922  TGTACA               T^GTACA
Bst1107I      993  GTATAC               GTA^TAC
BstAPI        999  GCAACTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I       993  GTATAC               GTA^TAC
Bsu36I       2937  CCTTAGG              CC^T[ACGT]AGG
CelII        1892  GCTCAGC              GC^T[ACGT]AGC
Eam1105I     3020  GACATTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     3687  AGCGCT               AGC^GCT
Eco81I       2937  CCTTAGG              CC^T[ACGT]AGG
FbaI          405  TGATCA               T^GATCA
Hin1I         160  GGCGTC               G[GA]^CG[CT]C
MfeI         5203  CAATTG               C^AATTG
MunI         5203  CAATTG               C^AATTG
NciI         2802  CCGGG                CC^[GC]GG
NdeI          471  CATATG               CA^TATG
PflFI        5024  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SpeI          434  ACTAGT               A^CTAGT
SspBI         922  TGTACA               T^GTACA
Tth111I      5024  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         2428  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccI          277  GTCTAC               GT^[AC][GT]AC
              993  GTATAC              
ApaI         4431  GGGCCC               GGGCC^C
             4661  GGGCCC              
AseI         5501  ATTAAT               AT^TAAT
             5875  ATTAAT              
AsnI         5501  ATTAAT               AT^TAAT
             5875  ATTAAT              
BalI           52  TGGCCA               TGG^CCA
             1638  TGGCCA              
BbvCI         816  CCTCAGC              CCTCAGC(-5/-2)
              951  CCTCAGC             
BsaI         3085  GAGACC               GGTCTC(1/5)
             1193  GGTCTC              
BsmBI        1202  CGTCTC               CGTCTC(1/5)
             3424  GAGACG              
BspLU11I     2820  ACATGT               A^CATGT
             3264  ACATGT              
BstYI         812  AGATCC               [GA]^GATC[CT]
             2884  GGATCT              
DsaI         1797  CCATGG               C^C[GA][CT]GG
             4427  CCATGG              
EaeI           52  TGGCCA               [CT]^GGCC[GA]
             1638  TGGCCA              
EcoNI         241  CCTCCAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4189  CCTTAGAGAGG         
EcoT22I      1603  ATGCAT               ATGCA^T
             3900  ATGCAT              
FauI          333  CCCGC                CCCGC(4/6)
             1765  CCCGC               
HaeII        3314  GGCGCT               [GA]GCGC^[CT]
             3687  AGCGCT              
HapII        1134  CCGG                 C^CGG
             2802  CCGG                
HpaI         3760  GTTAAC               GTT^AAC
             4467  GTTAAC              
HpaII        1134  CCGG                 C^CGG
             2802  CCGG                
MflI          812  AGATCC               [GA]^GATC[CT]
             2884  GGATCT              
MluNI          52  TGGCCA               TGG^CCA
             1638  TGGCCA              
MscI           52  TGGCCA               TGG^CCA
             1638  TGGCCA              
MspI         1134  CCGG                 C^CGG
             2802  CCGG                
NcoI         1797  CCATGG               C^CATGG
             4427  CCATGG              
NsiI         1603  ATGCAT               ATGCA^T
             3900  ATGCAT              
PshBI        5501  ATTAAT               AT^TAAT
             5875  ATTAAT              
ScaI         1970  AGTACT               AGT^ACT
             2945  AGTACT              
StuI         2910  AGGCCT               AGG^CCT
             5391  AGGCCT              
XhoII         812  AGATCC               [GA]^GATC[CT]
             2884  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   98 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        Asp700I     AvaII       
BanII       BbsI        BbvI        BfaI        BmyI        
BpmI        BpuAI       BsaJI       BseRI       BsgI        
BsiYI       BslI        BsmAI       BsmFI       BsmI        
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       BstXI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraII       DraIII      
EarI        EcoO109I    EcoRI       EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HgaI        HhaI        
HinP1I      HincII      HindII      HindIII     HinfI       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MnlI        MseI        
MslI        MspA1I      MvaI        MwoI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XmnI