##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Aor51HI ApaI AscI
Asp718I AviII BanI BbeI BcgI
BfrI BsaHI BseAI BsiWI Bsp1407I
BspDI BspEI BsrBI BsrFI BsrGI
BssHII Bst1107I BstXI BstZ17I Cfr10I
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I FseI FspI
HaeII Hin1I HpaI KasI KpnI
MluI MroI NaeI NarI NgoMIV
NotI NruI PacI PaeR7I PinAI
PmeI PshAI Psp1406I PvuI RsrII
SacI SapI SbfI SexAI SfiI
SfoI SgrAI SnaBI Sse8387I SspBI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AhdI 3236 GACTCCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 4733 ATTAAT AT^TAAT
AsnI 4733 ATTAAT AT^TAAT
AspEI 3236 GACTCCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2925 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 531 CCCGGG C^[CT]CG[GA]G
AvrII 2521 CCTAGG C^CTAGG
BalI 1075 TGGCCA TGG^CCA
BamHI 535 GGATCC G^GATCC
BbvCI 1616 CCTCAGC CCTCAGC(-5/-2)
BglII 3190 AGATCT A^GATCT
BlnI 2521 CCTAGG C^CTAGG
BsaWI 1314 ACCGGA [AT]^CCGG[AT]
BsiEI 586 CGGTCG CG[GA][CT]^CG
BsoBI 531 CCCGGG C^[CT]CG[GA]G
BspLU11I 5834 ACATGT A^CATGT
BssSI 4158 CTCGTG CACGAG(-5/-1)
BstAPI 5264 GCACTCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2780 TTCGAA TT^CGAA
BstEII 2093 GGTGACC G^GT[ACGT]ACC
BstPI 2093 GGTGACC G^GT[ACGT]ACC
EaeI 1075 TGGCCA [CT]^GGCC[GA]
Eam1105I 3236 GACTCCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1944 CCTTACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2093 GGTGACC G^GT[ACGT]ACC
EcoRV 3592 GATATC GAT^ATC
HgaI 606 GACGC GACGC(5/10)
KspI 575 CCGCGG CCGC^GG
MfeI 3066 CAATTG C^AATTG
MluNI 1075 TGGCCA TGG^CCA
MscI 1075 TGGCCA TGG^CCA
MunI 3066 CAATTG C^AATTG
NcoI 2192 CCATGG C^CATGG
NdeI 2268 CATATG CA^TATG
NheI 2558 GCTAGC G^CTAGC
NspV 2780 TTCGAA TT^CGAA
PflFI 2925 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3365 CCAACATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 4733 ATTAAT AT^TAAT
SacII 575 CCGCGG CCGC^GG
SalI 588 GTCGAC G^TCGAC
SfuI 2780 TTCGAA TT^CGAA
SmaI 531 CCCGGG CCC^GGG
Tth111I 2925 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3365 CCAACATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4586 TCTAGA T^CTAGA
XcmI 2020 CCATTTGGCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 531 CCCGGG C^CCGGG
XmaI 531 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 232 CGCG CG^CG
576 CGCG
AflIII 5834 ACATGT A^C[GA][CT]GT
6027 ACGTGT
Alw44I 2076 GTGCAC G^TGCAC
2457 GTGCAC
ApaLI 2076 GTGCAC G^TGCAC
2457 GTGCAC
AspHI 2076 GTGCAC G[AT]GC[AT]^C
2457 GTGCAC
BbrPI 2099 CACGTG CAC^GTG
4332 CACGTG
BciVI 1873 GGATAC GTATCC(6/5)
3613 GGATAC
BclI 1009 TGATCA T^GATCA
1563 TGATCA
BlpI 744 GCTTAGC GC^T[ACGT]AGC
4062 GCTTAGC
Bpu1102I 744 GCTTAGC GC^T[ACGT]AGC
4062 GCTTAGC
BsaAI 2099 CACGTG [CT]AC^GT[GA]
4332 CACGTG
BsaI 308 GAGACC GGTCTC(1/5)
4899 GAGACC
BsiHKAI 2076 GTGCAC G[AT]GC[AT]^C
2457 GTGCAC
BsmBI 3243 CGTCTC CGTCTC(1/5)
169 GAGACG
BsrDI 175 GCAATG GCAATG(2/0)
3212 GCAATG
BstUI 232 CGCG CG^CG
576 CGCG
CelII 744 GCTTAGC GC^T[ACGT]AGC
4062 GCTTAGC
CfoI 233 GCGC GCG^C
809 GCGC
DraII 1612 AGGACCT [GA]G^G[ACGT]CC[CT]
3134 AGGTCCC
EcoO109I 1612 AGGACCT [GA]G^G[ACGT]CC[CT]
3134 AGGTCCC
EcoT22I 3119 ATGCAT ATGCA^T
6194 ATGCAT
FauI 374 GCGGG CCCGC(4/6)
580 GCGGG
FbaI 1009 TGATCA T^GATCA
1563 TGATCA
HhaI 233 GCGC GCG^C
809 GCGC
HinP1I 233 GCGC G^CGC
809 GCGC
HincII 588 GTCGAC GT[CT]^[GA]AC
4289 GTTGAC
HindII 588 GTCGAC GT[CT]^[GA]AC
4289 GTTGAC
MvnI 232 CGCG CG^CG
576 CGCG
NsiI 3119 ATGCAT ATGCA^T
6194 ATGCAT
PmaCI 2099 CACGTG CAC^GTG
4332 CACGTG
PmlI 2099 CACGTG CAC^GTG
4332 CACGTG
PpuMI 1612 AGGACCT [GA]G^G[AT]CC[CT]
3134 AGGTCCC
SpeI 763 ACTAGT A^CTAGT
2687 ACTAGT
SphI 2112 GCATGC GCATG^C
5143 GCATGC
StuI 546 AGGCCT AGG^CCT
735 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI Asp700I
AvaII BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaBI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspHI BspMI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraI DsaI EarI
Eco81I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI