##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccII AclI AcyI AflII
AflIII AgeI AhdI Aor51HI ApaI
AscI AspEI AvaI AviII AvrII
BamHI BanII BbeI BbrPI BbvCI
BcnI BfrI BglI BlnI BsaAI
BsaHI BsgI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspLU11I BsrBI
BsrFI BsrGI BssHII Bst1107I BstUI
BstZ17I Cfr10I ClaI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoRV FseI
FspI Hin1I KasI KspI MluI
MvnI NaeI NarI NciI NcoI
NgoMIV NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI SacI SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI Sse8387I SspBI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 193 GGTACC G^GTACC
AccIII 4146 TCCGGA T^CCGGA
Alw44I 5066 GTGCAC G^TGCAC
ApaLI 5066 GTGCAC G^TGCAC
Asp718I 193 GGTACC G^GTACC
BcgI 4486 GCACTCCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5007 GGATAC GTATCC(6/5)
BclI 591 TGATCA T^GATCA
BglII 3925 AGATCT A^GATCT
BsaBI 586 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4146 TCCGGA [AT]^CCGG[AT]
BseAI 4146 TCCGGA T^CCGGA
BsmFI 899 GTCCC GGGAC(10/14)
BspEI 4146 TCCGGA T^CCGGA
BspMI 501 GCAGGT ACCTGC(4/8)
BstBI 1251 TTCGAA TT^CGAA
BstEII 3809 GGTCACC G^GT[ACGT]ACC
BstPI 3809 GGTCACC G^GT[ACGT]ACC
BstXI 4406 CCAGCTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 36 CGGACCG CG^G[AT]CCG
EcoO65I 3809 GGTCACC G^GT[ACGT]ACC
EcoRI 2785 GAATTC G^AATTC
EcoT22I 3993 ATGCAT ATGCA^T
FbaI 591 TGATCA T^GATCA
HaeII 208 GGCGCT [GA]GCGC^[CT]
HapII 4147 CCGG C^CGG
HgaI 1025 GACGC GACGC(5/10)
HpaII 4147 CCGG C^CGG
KpnI 193 GGTACC GGTAC^C
MamI 586 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3772 CAATTG C^AATTG
MroI 4146 TCCGGA T^CCGGA
MspI 4147 CCGG C^CGG
MunI 3772 CAATTG C^AATTG
NheI 1243 GCTAGC G^CTAGC
NsiI 3993 ATGCAT ATGCA^T
NspV 1251 TTCGAA TT^CGAA
PflMI 1840 CCAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 528 GACTCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 36 CGGACCG CG^G[AT]CCG
ScaI 3893 AGTACT AGT^ACT
SfuI 1251 TTCGAA TT^CGAA
StuI 2623 AGGCCT AGG^CCT
SwaI 4674 ATTTAAAT ATTT^AAAT
Van91I 1840 CCAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2569 CCATTCTACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AocI 2155 CCTAAGG CC^T[ACGT]AGG
4307 CCTGAGG
AseI 4599 ATTAAT AT^TAAT
5312 ATTAAT
AsnI 4599 ATTAAT AT^TAAT
5312 ATTAAT
AspHI 3365 GAGCAC G[AT]GC[AT]^C
5066 GTGCAC
AspI 1025 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4512 GACACTGTC
BalI 1278 TGGCCA TGG^CCA
4243 TGGCCA
BanI 193 GGTACC G^G[CT][GA]CC
5231 GGTGCC
BlpI 2305 GCTGAGC GC^T[ACGT]AGC
3362 GCTGAGC
BpmI 4489 CTCCAG CTGGAG(16/14)
5038 CTCCAG
Bpu1102I 2305 GCTGAGC GC^T[ACGT]AGC
3362 GCTGAGC
BsiHKAI 3365 GAGCAC G[AT]GC[AT]^C
5066 GTGCAC
BssSI 3011 CACGAG CACGAG(-5/-1)
530 CTCGTG
Bsu36I 2155 CCTAAGG CC^T[ACGT]AGG
4307 CCTGAGG
CelII 2305 GCTGAGC GC^T[ACGT]AGC
3362 GCTGAGC
DsaI 91 CCGTGG C^C[GA][CT]GG
120 CCGTGG
Eco81I 2155 CCTAAGG CC^T[ACGT]AGG
4307 CCTGAGG
FauI 9 GCGGG CCCGC(4/6)
1588 GCGGG
MluNI 1278 TGGCCA TGG^CCA
4243 TGGCCA
MscI 1278 TGGCCA TGG^CCA
4243 TGGCCA
NdeI 1370 CATATG CA^TATG
1811 CATATG
PflFI 1025 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4512 GACACTGTC
PshBI 4599 ATTAAT AT^TAAT
5312 ATTAAT
PvuII 2927 CAGCTG CAG^CTG
3026 CAGCTG
SapI 2347 GAAGAGC GCTCTTC(1/4)
2633 GAAGAGC
Tth111I 1025 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4512 GACACTGTC
XbaI 1021 TCTAGA T^CTAGA
1293 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AvaII
BbsI BbvI BfaI BmyI BpuAI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmI Bsp1286I BspHI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI EaeI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HhaI
HinP1I HincII HindII HindIII HinfI
HpaI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SpeI SphI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI