##################################
# NO CUT ENZYMES (   54 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AscI        Asp718I     AviII       AvrII       BfrI        
BlnI        BsiEI       BsiWI       Bsp1407I    BspHI       
BspLU11I    BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstZ17I     EagI        EclXI       Eco52I      
EcoT22I     FspI        HindIII     HpaI        KpnI        
MluI        NheI        NotI        NruI        NsiI        
NspV        PaeR7I      PmeI        PshAI       Psp1406I    
PvuI        RcaI        SalI        SbfI        SfiI        
SfuI        SgrAI       SpeI        SphI        Sse8387I    
SspBI       SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccIII        693  TCCGGA               T^CCGGA
AflIII       4835  ACACGT               A^C[GA][CT]GT
AgeI         3960  ACCGGT               A^CCGGT
Alw44I       3483  GTGCAC               G^TGCAC
Aor51HI        60  AGCGCT               AGC^GCT
ApaLI        3483  GTGCAC               G^TGCAC
AseI         5349  ATTAAT               AT^TAAT
AsnI         5349  ATTAAT               AT^TAAT
AspI         3576  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1032  TGGCCA               TGG^CCA
BamHI        4636  GGATCC               G^GATCC
BbrPI        4836  CACGTG               CAC^GTG
BciVI        3651  GTATCC               GTATCC(6/5)
BclI          236  TGATCA               T^GATCA
BglII        3564  AGATCT               A^GATCT
BsaBI        2122  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI         693  TCCGGA               T^CCGGA
BspDI        1310  ATCGAT               AT^CGAT
BspEI         693  TCCGGA               T^CCGGA
BssSI        5116  CTCGTG               CACGAG(-5/-1)
ClaI         1310  ATCGAT               AT^CGAT
CpoI         2070  CGGTCCG              CG^G[AT]CCG
Eco47III       60  AGCGCT               AGC^GCT
EcoRI        1178  GAATTC               G^AATTC
EcoRV        3475  GATATC               GAT^ATC
FbaI          236  TGATCA               T^GATCA
FseI          808  GGCCGGCC             GGCCGG^CC
MamI         2122  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         4751  CAATTG               C^AATTG
MluNI        1032  TGGCCA               TGG^CCA
MroI          693  TCCGGA               T^CCGGA
MscI         1032  TGGCCA               TGG^CCA
MslI         2962  CATCCCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI         4751  CAATTG               C^AATTG
NdeI         1686  CATATG               CA^TATG
PacI         5346  TTAATTAA             TTAAT^TAA
PflFI        3576  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        3960  ACCGGT               A^CCGGT
PmaCI        4836  CACGTG               CAC^GTG
PmlI         4836  CACGTG               CAC^GTG
PshBI        5349  ATTAAT               AT^TAAT
RsrII        2070  CGGTCCG              CG^G[AT]CCG
SacI         3774  GAGCTC               GAGCT^C
SapI         3221  GCTCTTC              GCTCTTC(1/4)
SexAI        4657  ACCTGGT              A^CC[AT]GGT
SnaBI        4491  TACGTA               TAC^GTA
SspI         5292  AATATT               AAT^ATT
Tth111I      3576  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AocI         1545  CCTCAGG              CC^T[ACGT]AGG
             2685  CCTCAGG             
BbvCI        1046  CCTCAGC              CCTCAGC(-5/-2)
             1863  CCTCAGC             
BlpI          442  GCTGAGC              GC^T[ACGT]AGC
             1516  GCTGAGC             
Bpu1102I      442  GCTGAGC              GC^T[ACGT]AGC
             1516  GCTGAGC             
BsmBI        3037  CGTCTC               CGTCTC(1/5)
             3270  GAGACG              
BsmI         1831  GAATGC               GAATGC(1/-1)
             1490  GCATTC              
BstEII       3343  GGTGACC              G^GT[ACGT]ACC
             4441  GGTGACC             
BstPI        3343  GGTGACC              G^GT[ACGT]ACC
             4441  GGTGACC             
Bsu36I       1545  CCTCAGG              CC^T[ACGT]AGG
             2685  CCTCAGG             
CelII         442  GCTGAGC              GC^T[ACGT]AGC
             1516  GCTGAGC             
DrdI          992  GACAGGACTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3431  GACCAGGGAGTC        
Eco81I       1545  CCTCAGG              CC^T[ACGT]AGG
             2685  CCTCAGG             
EcoO65I      3343  GGTGACC              G^GT[ACGT]ACC
             4441  GGTGACC             
HincII       2621  GTCAAC               GT[CT]^[GA]AC
             4756  GTTGAC              
HindII       2621  GTCAAC               GT[CT]^[GA]AC
             4756  GTTGAC              
NcoI         3096  CCATGG               C^CATGG
             3913  CCATGG              
ScaI         1161  AGTACT               AGT^ACT
             5258  AGTACT              
SmaI         2055  CCCGGG               CCC^GGG
             2666  CCCGGG              
XmaCI        2055  CCCGGG               C^CCGGG
             2666  CCCGGG              
XmaI         2055  CCCGGG               C^CCGGG
             2666  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  136 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AhdI        AluI        AlwI        AlwNI       ApaI        
ApoI        Asp700I     AspEI       AspHI       AvaI        
AvaII       BanI        BanII       BbeI        BbsI        
BbvI        BcgI        BcnI        BfaI        BglI        
BmyI        BpmI        BpuAI       BsaAI       BsaHI       
BsaI        BsaJI       BsaWI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrDI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraI        
DraII       DraIII      DsaI        EaeI        Eam1105I    
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     KspI        MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NdeII       NgoMIV      
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        SacII       
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfoI        SmlI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
XmnI