##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII AccI AccII AccIII AcyI
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BcgI
BciVI BcnI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BspHI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I FseI FspI HaeII HgaI
HhaI Hin1I HinP1I KasI KspI
MluI MroI MspA1I MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AclI 2216 AACGTT AA^CGTT
AocI 2921 CCTTAGG CC^T[ACGT]AGG
ApaI 582 GGGCCC GGGCC^C
AseI 4104 ATTAAT AT^TAAT
AsnI 4104 ATTAAT AT^TAAT
AspHI 3486 GAGCAC G[AT]GC[AT]^C
BalI 1759 TGGCCA TGG^CCA
BbsI 2367 GTCTTC GAAGAC(2/6)
BbvCI 4188 GCTGAGG CCTCAGC(-5/-2)
BpuAI 2367 GTCTTC GAAGAC(2/6)
BsaBI 494 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 887 GAGACC GGTCTC(1/5)
BsgI 608 CTGCAC GTGCAG(16/14)
BsiHKAI 3486 GAGCAC G[AT]GC[AT]^C
Bsp1407I 1195 TGTACA T^GTACA
BsrGI 1195 TGTACA T^GTACA
BstAPI 898 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2921 CCTTAGG CC^T[ACGT]AGG
EaeI 1759 TGGCCA [CT]^GGCC[GA]
Eco81I 2921 CCTTAGG CC^T[ACGT]AGG
EcoNI 2921 CCTTAGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2529 GAATTC G^AATTC
EcoT14I 4503 CCTTGG C^C[AT][AT]GG
FauI 2761 CCCGC CCCGC(4/6)
HindIII 4212 AAGCTT A^AGCTT
HpaI 221 GTTAAC GTT^AAC
MamI 494 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1759 TGGCCA TGG^CCA
MscI 1759 TGGCCA TGG^CCA
PshAI 855 GACAATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4104 ATTAAT AT^TAAT
Psp1406I 2216 AACGTT AA^CGTT
SbfI 630 CCTGCAGG CCTGCA^GG
SpeI 5380 ACTAGT A^CTAGT
Sse8387I 630 CCTGCAGG CCTGCA^GG
SspBI 1195 TGTACA T^GTACA
StyI 4503 CCTTGG C^C[AT][AT]GG
XcmI 952 CCAGCCATCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 1029 GGTACC G^GTACC
3630 GGTACC
AflIII 1192 ACATGT A^C[GA][CT]GT
1633 ACATGT
Asp718I 1029 GGTACC G^GTACC
3630 GGTACC
BglI 617 GCCTACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3351 GCCAGTTGGGC
BsmI 274 GAATGC GAATGC(1/-1)
4920 GAATGC
BspLU11I 1192 ACATGT A^CATGT
1633 ACATGT
BspMI 278 GCAGGT ACCTGC(4/8)
1954 GCAGGT
BstXI 1 CCAATTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2504 CCAATGAAATGG
DraIII 242 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
927 CACTAAGTG
EcoRV 498 GATATC GAT^ATC
1626 GATATC
EcoT22I 2586 ATGCAT ATGCA^T
4922 ATGCAT
HapII 1232 CCGG C^CGG
1243 CCGG
HincII 221 GTTAAC GT[CT]^[GA]AC
4254 GTTGAC
HindII 221 GTTAAC GT[CT]^[GA]AC
4254 GTTGAC
HpaII 1232 CCGG C^CGG
1243 CCGG
KpnI 1029 GGTACC GGTAC^C
3630 GGTACC
MfeI 857 CAATTG C^AATTG
3936 CAATTG
MspI 1232 CCGG C^CGG
1243 CCGG
MunI 857 CAATTG C^AATTG
3936 CAATTG
NsiI 2586 ATGCAT ATGCA^T
4922 ATGCAT
PpuMI 648 AGGTCCC [GA]G^G[AT]CC[CT]
3692 GGGTCCT
PstI 631 CTGCAG CTGCA^G
2619 CTGCAG
SphI 907 GCATGC GCATG^C
3196 GCATGC
StuI 615 AGGCCT AGG^CCT
2330 AGGCCT
TaqI 1702 TCGA T^CGA
2566 TCGA
TthHB8I 1702 TCGA T^CGA
2566 TCGA
XbaI 2717 TCTAGA T^CTAGA
2868 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI AlwNI ApoI Asp700I AvaII
BanI BanII BbvI BclI BfaI
BfrI BmyI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRII FbaI
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XhoII XmnI