##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AclI AflII AgeI AscI
Asp718I AspI AviII AvrII BbeI
BbrPI BcgI BfrI BglI BlnI
BsaAI BsiWI Bsp1407I BspDI BsrBI
BsrGI BssHII BstBI BstEII BstPI
ClaI DsaI EagI EclXI Eco52I
EcoO65I FseI FspI HpaI KasI
KpnI KspI MluI NarI NcoI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
Psp1406I SacII ScaI SfiI SfoI
SfuI SgrAI SnaBI SphI SspBI
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Alw44I 2097 GTGCAC G^TGCAC
AocI 4849 CCTTAGG CC^T[ACGT]AGG
Aor51HI 5399 AGCGCT AGC^GCT
ApaI 147 GGGCCC GGGCC^C
ApaLI 2097 GTGCAC G^TGCAC
AseI 5451 ATTAAT AT^TAAT
AsnI 5451 ATTAAT AT^TAAT
BbsI 2262 GAAGAC GAAGAC(2/6)
BbvCI 5182 CCTCAGC CCTCAGC(-5/-2)
BciVI 5120 GGATAC GTATCC(6/5)
BpuAI 2262 GAAGAC GAAGAC(2/6)
BsiEI 11 CGATCG CG[GA][CT]^CG
BspHI 4312 TCATGA T^CATGA
BspLU11I 478 ACATGT A^CATGT
BsrDI 3025 GCAATG GCAATG(2/0)
BssSI 2100 CACGAG CACGAG(-5/-1)
Bsu36I 4849 CCTTAGG CC^T[ACGT]AGG
CpoI 86 CGGACCG CG^G[AT]CCG
DraIII 2157 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 777 GACTTTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5399 AGCGCT AGC^GCT
Eco81I 4849 CCTTAGG CC^T[ACGT]AGG
EcoNI 2428 CCTTGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2324 GAATTC G^AATTC
NaeI 1537 GCCGGC GCC^GGC
NdeI 892 CATATG CA^TATG
NgoMIV 1537 GCCGGC G^CCGGC
NheI 2646 GCTAGC G^CTAGC
PshAI 5685 GACTTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5451 ATTAAT AT^TAAT
PvuI 11 CGATCG CGAT^CG
RcaI 4312 TCATGA T^CATGA
RsrII 86 CGGACCG CG^G[AT]CCG
SapI 4352 GAAGAGC GCTCTTC(1/4)
SbfI 327 CCTGCAGG CCTGCA^GG
SexAI 3058 ACCTGGT A^CC[AT]GGT
SmaI 150 CCCGGG CCC^GGG
Sse8387I 327 CCTGCAGG CCTGCA^GG
StuI 1797 AGGCCT AGG^CCT
XbaI 4427 TCTAGA T^CTAGA
XmaCI 150 CCCGGG C^CCGGG
XmaI 150 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AatII 2987 GACGTC GACGT^C
3215 GACGTC
AccIII 2398 TCCGGA T^CCGGA
2707 TCCGGA
AcyI 2987 GACGTC G[GA]^CG[CT]C
3215 GACGTC
AflIII 478 ACATGT A^C[GA][CT]GT
634 ACACGT
AhdI 909 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3553 GACTGAATGTC
AspEI 909 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3553 GACTGAATGTC
BamHI 108 GGATCC G^GATCC
2523 GGATCC
BclI 613 TGATCA T^GATCA
3511 TGATCA
BlpI 21 GCTCAGC GC^T[ACGT]AGC
792 GCTCAGC
Bpu1102I 21 GCTCAGC GC^T[ACGT]AGC
792 GCTCAGC
BsaBI 103 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2667 GATGAGGATC
BsaHI 2987 GACGTC G[GA]^CG[CT]C
3215 GACGTC
BseAI 2398 TCCGGA T^CCGGA
2707 TCCGGA
BspEI 2398 TCCGGA T^CCGGA
2707 TCCGGA
BsrFI 1537 GCCGGC [GA]^CCGG[CT]
1897 ACCGGC
Bst1107I 3207 GTATAC GTA^TAC
3697 GTATAC
BstAPI 945 GCAGAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1655 GCAGCTACTGC
BstXI 934 CCATTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4114 CCACGTCTGTGG
BstZ17I 3207 GTATAC GTA^TAC
3697 GTATAC
CelII 21 GCTCAGC GC^T[ACGT]AGC
792 GCTCAGC
Cfr10I 1537 GCCGGC [GA]^CCGG[CT]
1897 ACCGGC
Eam1105I 909 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3553 GACTGAATGTC
EcoRV 1940 GATATC GAT^ATC
3945 GATATC
EcoT22I 4215 ATGCAT ATGCA^T
4736 ATGCAT
FbaI 613 TGATCA T^GATCA
3511 TGATCA
Hin1I 2987 GACGTC G[GA]^CG[CT]C
3215 GACGTC
HindIII 221 AAGCTT A^AGCTT
5259 AAGCTT
MamI 103 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2667 GATGAGGATC
MfeI 1110 CAATTG C^AATTG
1701 CAATTG
MroI 2398 TCCGGA T^CCGGA
2707 TCCGGA
MunI 1110 CAATTG C^AATTG
1701 CAATTG
NsiI 4215 ATGCAT ATGCA^T
4736 ATGCAT
NspI 478 ACATGT [GA]CATG^[CT]
970 ACATGC
PflMI 2156 CCACCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3128 CCAGGAGATGG
SalI 1307 GTCGAC G^TCGAC
2638 GTCGAC
SmlI 1170 CTCAAG C^T[CT][GA]AG
5424 CTCAAG
Van91I 2156 CCACCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3128 CCAGGAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BglII
BmyI BpmI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SpeI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI