################################## # NO CUT ENZYMES ( 47 enzymes) # ################################## AatII AflII AgeI AhdI AocI Aor51HI AspEI AspI AviII BbrPI BciVI BfrI BsaAI BsiWI BspDI Bsu36I ClaI CpoI DrdI Eam1105I Eco47III Eco81I EcoRI EcoT22I FseI FspI HpaI MluI NotI NsiI PacI PflFI PinAI PmaCI PmeI PmlI PvuI RsrII SalI SapI SexAI SfiI SnaBI SpeI SphI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AclI 4642 AACGTT AA^CGTT AscI 306 GGCGCGCC GG^CGCGCC Asp700I 5542 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BclI 4273 TGATCA T^GATCA BglII 2234 AGATCT A^GATCT BsmI 4777 GCATTC GAATGC(1/-1) Bsp1407I 5510 TGTACA T^GTACA BspLU11I 4010 ACATGT A^CATGT BspMI 5020 GCAGGT ACCTGC(4/8) BsrGI 5510 TGTACA T^GTACA BssSI 2670 CTCGTG CACGAG(-5/-1) Bst1107I 4164 GTATAC GTA^TAC BstAPI 1769 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 944 TTCGAA TT^CGAA BstEII 5383 GGTTACC G^GT[ACGT]ACC BstPI 5383 GGTTACC G^GT[ACGT]ACC BstZ17I 4164 GTATAC GTA^TAC DraIII 3422 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 2684 CCTCTTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 5383 GGTTACC G^GT[ACGT]ACC FbaI 4273 TGATCA T^GATCA HincII 753 GTTGAC GT[CT]^[GA]AC HindII 753 GTTGAC GT[CT]^[GA]AC HindIII 4282 AAGCTT A^AGCTT NdeI 5798 CATATG CA^TATG NheI 5803 GCTAGC G^CTAGC NruI 852 TCGCGA TCG^CGA NspV 944 TTCGAA TT^CGAA PaeR7I 1 CTCGAG C^TCGAG Psp1406I 4642 AACGTT AA^CGTT SbfI 1766 CCTGCAGG CCTGCA^GG ScaI 4473 AGTACT AGT^ACT SfuI 944 TTCGAA TT^CGAA SgrAI 310 CGCCGGCG C[GA]^CCGG[CT]G SmlI 1 CTCGAG C^T[CT][GA]AG Sse8387I 1766 CCTGCAGG CCTGCA^GG SspBI 5510 TGTACA T^GTACA XcmI 5026 CCAATTCTGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1 CTCGAG C^TCGAG XmnI 5542 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### Acc65I 3274 GGTACC G^GTACC 5518 GGTACC AccI 2545 GTAGAC GT^[AC][GT]AC 4164 GTATAC AflIII 122 ACACGT A^C[GA][CT]GT 4010 ACATGT Alw44I 993 GTGCAC G^TGCAC 4799 GTGCAC ApaLI 993 GTGCAC G^TGCAC 4799 GTGCAC AseI 553 ATTAAT AT^TAAT 5116 ATTAAT AsnI 553 ATTAAT AT^TAAT 5116 ATTAAT Asp718I 3274 GGTACC G^GTACC 5518 GGTACC BamHI 63 GGATCC G^GATCC 465 GGATCC BbsI 4653 GAAGAC GAAGAC(2/6) 4370 GTCTTC BbvCI 534 GCTGAGG CCTCAGC(-5/-2) 1390 GCTGAGG BglI 311 GCCGGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1004 GCCCCGAGGGC BpuAI 4653 GAAGAC GAAGAC(2/6) 4370 GTCTTC BsaBI 174 GATCTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3516 GATAGAAATC BsgI 1776 CTGCAC GTGCAG(16/14) 1465 GTGCAG BsiEI 8 CGGCCG CG[GA][CT]^CG 1099 CGGCCG BspHI 3512 TCATGA T^CATGA 4996 TCATGA BsrDI 2621 CATTGC GCAATG(2/0) 4681 GCAATG BssHII 307 GCGCGC G^CGCGC 665 GCGCGC BstXI 4670 CCATACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4885 CCATACTGATGG EagI 8 CGGCCG C^GGCCG 1099 CGGCCG EclXI 8 CGGCCG C^GGCCG 1099 CGGCCG Eco52I 8 CGGCCG C^GGCCG 1099 CGGCCG EcoRV 2938 GATATC GAT^ATC 4631 GATATC KpnI 3274 GGTACC GGTAC^C 5518 GGTACC KspI 103 CCGCGG CCGC^GG 1037 CCGCGG MamI 174 GATCTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3516 GATAGAAATC MfeI 3279 CAATTG C^AATTG 3497 CAATTG MunI 3279 CAATTG C^AATTG 3497 CAATTG NcoI 4148 CCATGG C^CATGG 5565 CCATGG PpuMI 1303 GGGACCT [GA]G^G[AT]CC[CT] 2006 AGGTCCT PshAI 3389 GACATGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3413 GACATCTGTC PshBI 553 ATTAAT AT^TAAT 5116 ATTAAT PstI 1544 CTGCAG CTGCA^G 1767 CTGCAG RcaI 3512 TCATGA T^CATGA 4996 TCATGA SacI 228 GAGCTC GAGCT^C 1532 GAGCTC SacII 103 CCGCGG CCGC^GG 1037 CCGCGG StuI 602 AGGCCT AGG^CCT 840 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 133 enzymes) # ######################################### AccII AccIII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII AvrII BalI BanI BanII BbeI BbvI BcgI BcnI BfaI BlnI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BsaWI BseAI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspEI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XhoII XmaCI XmaI