##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AatII AflII AgeI AhdI AocI
Aor51HI AspEI AspI AviII BbrPI
BciVI BfrI BsaAI BsiWI BspDI
Bsu36I ClaI CpoI DrdI Eam1105I
Eco47III Eco81I EcoRI EcoT22I FseI
FspI HpaI MluI NotI NsiI
PacI PflFI PinAI PmaCI PmeI
PmlI PvuI RsrII SalI SapI
SexAI SfiI SnaBI SpeI SphI
SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AclI 4642 AACGTT AA^CGTT
AscI 306 GGCGCGCC GG^CGCGCC
Asp700I 5542 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BclI 4273 TGATCA T^GATCA
BglII 2234 AGATCT A^GATCT
BsmI 4777 GCATTC GAATGC(1/-1)
Bsp1407I 5510 TGTACA T^GTACA
BspLU11I 4010 ACATGT A^CATGT
BspMI 5020 GCAGGT ACCTGC(4/8)
BsrGI 5510 TGTACA T^GTACA
BssSI 2670 CTCGTG CACGAG(-5/-1)
Bst1107I 4164 GTATAC GTA^TAC
BstAPI 1769 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 944 TTCGAA TT^CGAA
BstEII 5383 GGTTACC G^GT[ACGT]ACC
BstPI 5383 GGTTACC G^GT[ACGT]ACC
BstZ17I 4164 GTATAC GTA^TAC
DraIII 3422 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 2684 CCTCTTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 5383 GGTTACC G^GT[ACGT]ACC
FbaI 4273 TGATCA T^GATCA
HincII 753 GTTGAC GT[CT]^[GA]AC
HindII 753 GTTGAC GT[CT]^[GA]AC
HindIII 4282 AAGCTT A^AGCTT
NdeI 5798 CATATG CA^TATG
NheI 5803 GCTAGC G^CTAGC
NruI 852 TCGCGA TCG^CGA
NspV 944 TTCGAA TT^CGAA
PaeR7I 1 CTCGAG C^TCGAG
Psp1406I 4642 AACGTT AA^CGTT
SbfI 1766 CCTGCAGG CCTGCA^GG
ScaI 4473 AGTACT AGT^ACT
SfuI 944 TTCGAA TT^CGAA
SgrAI 310 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 1 CTCGAG C^T[CT][GA]AG
Sse8387I 1766 CCTGCAGG CCTGCA^GG
SspBI 5510 TGTACA T^GTACA
XcmI 5026 CCAATTCTGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1 CTCGAG C^TCGAG
XmnI 5542 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 3274 GGTACC G^GTACC
5518 GGTACC
AccI 2545 GTAGAC GT^[AC][GT]AC
4164 GTATAC
AflIII 122 ACACGT A^C[GA][CT]GT
4010 ACATGT
Alw44I 993 GTGCAC G^TGCAC
4799 GTGCAC
ApaLI 993 GTGCAC G^TGCAC
4799 GTGCAC
AseI 553 ATTAAT AT^TAAT
5116 ATTAAT
AsnI 553 ATTAAT AT^TAAT
5116 ATTAAT
Asp718I 3274 GGTACC G^GTACC
5518 GGTACC
BamHI 63 GGATCC G^GATCC
465 GGATCC
BbsI 4653 GAAGAC GAAGAC(2/6)
4370 GTCTTC
BbvCI 534 GCTGAGG CCTCAGC(-5/-2)
1390 GCTGAGG
BglI 311 GCCGGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1004 GCCCCGAGGGC
BpuAI 4653 GAAGAC GAAGAC(2/6)
4370 GTCTTC
BsaBI 174 GATCTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3516 GATAGAAATC
BsgI 1776 CTGCAC GTGCAG(16/14)
1465 GTGCAG
BsiEI 8 CGGCCG CG[GA][CT]^CG
1099 CGGCCG
BspHI 3512 TCATGA T^CATGA
4996 TCATGA
BsrDI 2621 CATTGC GCAATG(2/0)
4681 GCAATG
BssHII 307 GCGCGC G^CGCGC
665 GCGCGC
BstXI 4670 CCATACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4885 CCATACTGATGG
EagI 8 CGGCCG C^GGCCG
1099 CGGCCG
EclXI 8 CGGCCG C^GGCCG
1099 CGGCCG
Eco52I 8 CGGCCG C^GGCCG
1099 CGGCCG
EcoRV 2938 GATATC GAT^ATC
4631 GATATC
KpnI 3274 GGTACC GGTAC^C
5518 GGTACC
KspI 103 CCGCGG CCGC^GG
1037 CCGCGG
MamI 174 GATCTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3516 GATAGAAATC
MfeI 3279 CAATTG C^AATTG
3497 CAATTG
MunI 3279 CAATTG C^AATTG
3497 CAATTG
NcoI 4148 CCATGG C^CATGG
5565 CCATGG
PpuMI 1303 GGGACCT [GA]G^G[AT]CC[CT]
2006 AGGTCCT
PshAI 3389 GACATGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3413 GACATCTGTC
PshBI 553 ATTAAT AT^TAAT
5116 ATTAAT
PstI 1544 CTGCAG CTGCA^G
1767 CTGCAG
RcaI 3512 TCATGA T^CATGA
4996 TCATGA
SacI 228 GAGCTC GAGCT^C
1532 GAGCTC
SacII 103 CCGCGG CCGC^GG
1037 CCGCGG
StuI 602 AGGCCT AGG^CCT
840 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII AvrII
BalI BanI BanII BbeI BbvI
BcgI BcnI BfaI BlnI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspEI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XhoII XmaCI XmaI