##################################
# NO CUT ENZYMES (   73 enzymes) #
##################################

AatII       AccIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        AspEI       AspI        AviII       AvrII       
BbeI        BbrPI       BbvCI       BglI        BglII       
BlnI        BsaAI       BsaBI       BseAI       BspDI       
BspEI       BssHII      BssSI       Bst1107I    BstBI       
BstZ17I     ClaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      FseI        FspI        KasI        
KspI        MamI        MluI        MroI        NarI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshBI       PvuI        SacI        SacII       SbfI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SwaI        Tth111I     
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AclI         1569  AACGTT               AA^CGTT
AcyI         3556  GGCGTC               G[GA]^CG[CT]C
AflIII       3109  ACATGT               A^C[GA][CT]GT
AocI         3172  CCTGAGG              CC^T[ACGT]AGG
AspHI        4146  GAGCAC               G[AT]GC[AT]^C
AvaI         4477  CCCGAG               C^[CT]CG[GA]G
BcgI         3683  GCAAGGAAGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI         2146  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2146  GCTGAGC              GC^T[ACGT]AGC
BsaHI        3556  GGCGTC               G[GA]^CG[CT]C
BsaWI        2923  ACCGGA               [AT]^CCGG[AT]
BsiEI        1030  CGACCG               CG[GA][CT]^CG
BsiHKAI      4146  GAGCAC               G[AT]GC[AT]^C
BsiWI        3954  CGTACG               C^GTACG
BsoBI        4477  CCCGAG               C^[CT]CG[GA]G
BspHI         981  TCATGA               T^CATGA
BspLU11I     3109  ACATGT               A^CATGT
BsrBI        2149  GAGCGG               CCGCTC(-3/-3)
BstEII       1109  GGTCACC              G^GT[ACGT]ACC
BstPI        1109  GGTCACC              G^GT[ACGT]ACC
Bsu36I       3172  CCTGAGG              CC^T[ACGT]AGG
CelII        2146  GCTGAGC              GC^T[ACGT]AGC
CpoI          504  CGGACCG              CG^G[AT]CCG
DraIII       3137  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         3550  GACTGTGGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       3172  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      1109  GGTCACC              G^GT[ACGT]ACC
EcoRI        1378  GAATTC               G^AATTC
EcoRV        5664  GATATC               GAT^ATC
EcoT22I      1091  ATGCAT               ATGCA^T
Hin1I        3556  GGCGTC               G[GA]^CG[CT]C
HpaI         4590  GTTAAC               GTT^AAC
NaeI         2276  GCCGGC               GCC^GGC
NgoMIV       2276  GCCGGC               G^CCGGC
NheI         2167  GCTAGC               G^CTAGC
NsiI         1091  ATGCAT               ATGCA^T
PshAI        3859  GACCAAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     1569  AACGTT               AA^CGTT
RcaI          981  TCATGA               T^CATGA
RsrII         504  CGGACCG              CG^G[AT]CCG
SalI         3559  GTCGAC               G^TCGAC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

Acc65I       1277  GGTACC               G^GTACC
             5552  GGTACC              
AccII         472  CGCG                 CG^CG
             2884  CGCG                
AflII         783  CTTAAG               C^TTAAG
             5871  CTTAAG              
Asp718I      1277  GGTACC               G^GTACC
             5552  GGTACC              
BalI         2866  TGGCCA               TGG^CCA
             3945  TGGCCA              
BamHI        1081  GGATCC               G^GATCC
             6471  GGATCC              
BanII        2596  GAGCCC               G[GA]GC[CT]^C
             3586  GAGCCC              
BciVI        1645  GGATAC               GTATCC(6/5)
              558  GTATCC              
BcnI         2268  CCCGG                CC^[GC]GG
             5275  CCCGG               
BfrI          783  CTTAAG               C^TTAAG
             5871  CTTAAG              
BsrFI        1274  GCCGGT               [GA]^CCGG[CT]
             2276  GCCGGC              
BstUI         472  CGCG                 CG^CG
             2884  CGCG                
BstXI        4457  CCATGCAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             6160  CCAAATACTTGG        
Cfr10I       1274  GCCGGT               [GA]^CCGG[CT]
             2276  GCCGGC              
EcoNI        1650  CCTCTCTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             5884  CCTTGATTAGG         
KpnI         1277  GGTACC               GGTAC^C
             5552  GGTACC              
MfeI          341  CAATTG               C^AATTG
             5435  CAATTG              
MluNI        2866  TGGCCA               TGG^CCA
             3945  TGGCCA              
MscI         2866  TGGCCA               TGG^CCA
             3945  TGGCCA              
MunI          341  CAATTG               C^AATTG
             5435  CAATTG              
MvnI          472  CGCG                 CG^CG
             2884  CGCG                
NciI         2268  CCCGG                CC^[GC]GG
             5275  CCCGG               
NdeI          886  CATATG               CA^TATG
             5219  CATATG              
SexAI        1318  ACCTGGT              A^CC[AT]GGT
             3138  ACCAGGT             
SphI         4322  GCATGC               GCATG^C
             5794  GCATGC              
XbaI          283  TCTAGA               T^CTAGA
             1765  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  118 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        Asp700I     AvaII       
BanI        BbsI        BbvI        BclI        BfaI        
BmyI        BpmI        BpuAI       BsaI        BsaJI       
BseRI       BsgI        BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsmI        Bsp1286I    Bsp1407I    
BspMI       BsrDI       BsrGI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        FbaI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HincII      HindII      HindIII     
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        SapI        
Sau3AI      Sau96I      ScaI        ScrFI       SfaNI       
SfcI        SmlI        SspBI       SspI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoII       XmnI