##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AclI AhdI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AvrII BamHI
BanII BcgI BglI BlnI BlpI
Bpu1102I BsiEI BsiWI BsmBI BssHII
BstBI Bsu36I CelII CpoI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoRI EcoRV FseI KpnI
KspI MfeI MluI MunI NotI
NruI NspV PacI PaeR7I PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
SfiI SfuI SgrAI SnaBI Sse8387I
StuI XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccI 3679 GTATAC GT^[AC][GT]AC
AflII 824 CTTAAG C^TTAAG
AgeI 2436 ACCGGT A^CCGGT
Aor51HI 2049 AGCGCT AGC^GCT
BbeI 22 GGCGCC GGCGC^C
BbrPI 4525 CACGTG CAC^GTG
BbvCI 1633 CCTCAGC CCTCAGC(-5/-2)
BfrI 824 CTTAAG C^TTAAG
BsaAI 4525 CACGTG [CT]AC^GT[GA]
BsaBI 3008 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BssSI 6010 CTCGTG CACGAG(-5/-1)
Bst1107I 3679 GTATAC GTA^TAC
BstEII 5419 GGTTACC G^GT[ACGT]ACC
BstPI 5419 GGTTACC G^GT[ACGT]ACC
BstXI 1212 CCAAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3679 GTATAC GTA^TAC
Eco47III 2049 AGCGCT AGC^GCT
EcoO65I 5419 GGTTACC G^GT[ACGT]ACC
HpaI 5686 GTTAAC GTT^AAC
KasI 22 GGCGCC G^GCGCC
MamI 3008 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 25 GCCGGC GCC^GGC
NarI 22 GGCGCC GG^CGCC
NgoMIV 25 GCCGGC G^CCGGC
NheI 5226 GCTAGC G^CTAGC
PflMI 3256 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2436 ACCGGT A^CCGGT
PmaCI 4525 CACGTG CAC^GTG
PmlI 4525 CACGTG CAC^GTG
ScaI 6076 AGTACT AGT^ACT
SfoI 22 GGCGCC GGC^GCC
SpeI 2944 ACTAGT A^CTAGT
SphI 4059 GCATGC GCATG^C
SwaI 4426 ATTTAAAT ATTT^AAAT
Van91I 3256 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2254 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AspHI 1010 GAGCAC G[AT]GC[AT]^C
1673 GTGCTC
AspI 382 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4457 GACTGAGTC
AviII 2613 TGCGCA TGC^GCA
5505 TGCGCA
BalI 2550 TGGCCA TGG^CCA
2766 TGGCCA
BanI 22 GGCGCC G^G[CT][GA]CC
3975 GGCACC
BglII 115 AGATCT A^GATCT
700 AGATCT
BsgI 1998 CTGCAC GTGCAG(16/14)
4004 CTGCAC
BsiHKAI 1010 GAGCAC G[AT]GC[AT]^C
1673 GTGCTC
BspDI 3051 ATCGAT AT^CGAT
5931 ATCGAT
BspHI 454 TCATGA T^CATGA
4453 TCATGA
BspLU11I 4292 ACATGT A^CATGT
4472 ACATGT
BsrBI 1005 GAGCGG CCGCTC(-3/-3)
3266 GAGCGG
BsrDI 2863 CATTGC GCAATG(2/0)
5785 GCAATG
ClaI 3051 ATCGAT AT^CGAT
5931 ATCGAT
EcoNI 2452 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5324 CCTTTATGAGG
FspI 2613 TGCGCA TGC^GCA
5505 TGCGCA
HaeII 22 GGCGCC [GA]GCGC^[CT]
2049 AGCGCT
MluNI 2550 TGGCCA TGG^CCA
2766 TGGCCA
MscI 2550 TGGCCA TGG^CCA
2766 TGGCCA
NcoI 546 CCATGG C^CATGG
3530 CCATGG
NdeI 2525 CATATG CA^TATG
3118 CATATG
PflFI 382 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4457 GACTGAGTC
PstI 725 CTGCAG CTGCA^G
3205 CTGCAG
RcaI 454 TCATGA T^CATGA
4453 TCATGA
SexAI 654 ACCAGGT A^CC[AT]GGT
5117 ACCAGGT
SmaI 49 CCCGGG CCC^GGG
96 CCCGGG
Tth111I 382 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4457 GACTGAGTC
XmaCI 49 CCCGGG C^CCGGG
96 CCCGGG
XmaI 49 CCCGGG C^CCGGG
96 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI Asp700I AvaI AvaII BbsI
BbvI BciVI BclI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspEI BspMI BsrFI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I EcoT22I FauI FbaI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MroI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI