##################################
# NO CUT ENZYMES (   58 enzymes) #
##################################

AccIII      AclI        AgeI        Alw44I      ApaI        
ApaLI       AseI        AsnI        AspI        AvrII       
BbrPI       BbvCI       BcgI        BlnI        BsaAI       
BsaBI       BseAI       BsiWI       Bsp1407I    BspEI       
BsrGI       Bst1107I    BstZ17I     CpoI        DraI        
DraIII      EcoT22I     KspI        MamI        MluI        
MroI        NdeI        NotI        NruI        NsiI        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SfiI        SgrAI       
SmaI        SnaBI       SspBI       SwaI        Tth111I     
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AatII        2898  GACGTC               GACGT^C
AflII        4550  CTTAAG               C^TTAAG
AflIII       1004  ACATGT               A^C[GA][CT]GT
AhdI         4133  GACAGGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         2766  CCTGAGG              CC^T[ACGT]AGG
AscI          501  GGCGCGCC             GG^CGCGCC
AspEI        4133  GACAGGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         156  TGCGCA               TGC^GCA
BalI         1226  TGGCCA               TGG^CCA
BamHI           1  GGATCC               G^GATCC
BbeI          233  GGCGCC               GGCGC^C
BciVI        4784  GGATAC               GTATCC(6/5)
BfrI         4550  CTTAAG               C^TTAAG
BglII        3570  AGATCT               A^GATCT
BlpI         2149  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2149  GCTCAGC              GC^T[ACGT]AGC
BsmI         4211  GAATGC               GAATGC(1/-1)
BspDI         842  ATCGAT               AT^CGAT
BspLU11I     1004  ACATGT               A^CATGT
BsrFI         284  GCCGGC               [GA]^CCGG[CT]
BssSI        2296  CACGAG               CACGAG(-5/-1)
BstAPI        894  GCAAAAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        2432  TTCGAA               TT^CGAA
BstEII        597  GGTCACC              G^GT[ACGT]ACC
BstPI         597  GGTCACC              G^GT[ACGT]ACC
BstXI        2774  CCAAAGGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       2766  CCTGAGG              CC^T[ACGT]AGG
CelII        2149  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        284  GCCGGC               [GA]^CCGG[CT]
ClaI          842  ATCGAT               AT^CGAT
DrdI         1003  GACATGTCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     4133  GACAGGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       2766  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       597  GGTCACC              G^GT[ACGT]ACC
EcoRI        1818  GAATTC               G^AATTC
EcoRV        4576  GATATC               GAT^ATC
FseI          283  GGCCGGCC             GGCCGG^CC
FspI          156  TGCGCA               TGC^GCA
HpaI         5439  GTTAAC               GTT^AAC
KasI          233  GGCGCC               G^GCGCC
MfeI         5242  CAATTG               C^AATTG
MluNI        1226  TGGCCA               TGG^CCA
MscI         1226  TGGCCA               TGG^CCA
MunI         5242  CAATTG               C^AATTG
NaeI          284  GCCGGC               GCC^GGC
NarI          233  GGCGCC               GG^CGCC
NcoI         1119  CCATGG               C^CATGG
NgoMIV        284  GCCGGC               G^CCGGC
NheI         1408  GCTAGC               G^CTAGC
NspV         2432  TTCGAA               TT^CGAA
SalI         1263  GTCGAC               G^TCGAC
ScaI         5205  AGTACT               AGT^ACT
SexAI        2048  ACCAGGT              A^CC[AT]GGT
SfoI          233  GGCGCC               GGC^GCC
SfuI         2432  TTCGAA               TT^CGAA
SpeI         3741  ACTAGT               A^CTAGT
SphI          153  GCATGC               GCATG^C
SspI         3191  AATATT               AAT^ATT
XbaI         2640  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

Asp700I      3898  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4925  GAACAGCTTC          
BglI          447  GCCCGAGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2404  GCCTGGGAGGC         
BsiEI         282  CGGCCG               CG[GA][CT]^CG
              286  CGGCCG              
BsrBI        1768  CCGCTC               CCGCTC(-3/-3)
             2611  GAGCGG              
BssHII        487  GCGCGC               G^CGCGC
              502  GCGCGC              
DsaI         1119  CCATGG               C^C[GA][CT]GG
             1158  CCGTGG              
EagI          282  CGGCCG               C^GGCCG
              286  CGGCCG              
EclXI         282  CGGCCG               C^GGCCG
              286  CGGCCG              
Eco52I        282  CGGCCG               C^GGCCG
              286  CGGCCG              
PflMI         588  CCATTGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2220  CCAGAGGGTGG         
SbfI         1728  CCTGCAGG             CCTGCA^GG
             5053  CCTGCAGG            
Sse8387I     1728  CCTGCAGG             CCTGCA^GG
             5053  CCTGCAGG            
Van91I        588  CCATTGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2220  CCAGAGGGTGG         
XcmI         2103  CCAAAGCCAGCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4284  CCACTGAGGTCATGG     
XmnI         3898  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4925  GAACAGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  126 enzymes) #
#########################################

Acc65I      AccI        AccII       AciI        AcsI        
AcyI        AfaI        AluI        AlwI        AlwNI       
Aor51HI     ApoI        Asp718I     AspHI       AvaI        
AvaII       BanI        BanII       BbsI        BbvI        
BclI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaHI       BsaI        BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspHI       BspMI       BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EaeI        EarI        Eco47III    EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        FbaI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HinfI       HpaII       HphI        ItaI        
KpnI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PleI        PpuMI       PstI        PvuII       
RcaI        RsaI        SacI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII