##################################
# NO CUT ENZYMES (   71 enzymes) #
##################################

Acc65I      AclI        AhdI        Aor51HI     AscI        
Asp718I     AspEI       AviII       BbeI        BbrPI       
BcgI        BglII       BsaAI       BsaBI       BsiEI       
BsiWI       BspDI       BsrBI       BssHII      BssSI       
Bst1107I    BstBI       BstXI       BstZ17I     ClaI        
CpoI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoT22I     FspI        KasI        
KpnI        KspI        MamI        MluI        NarI        
NdeI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PmaCI       PmeI        PmlI        
Psp1406I    PvuI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       Sse8387I    
StuI        SwaI        XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AatII        4213  GACGTC               GACGT^C
AccI         3823  GTCTAC               GT^[AC][GT]AC
AccII        3616  CGCG                 CG^CG
AccIII       2537  TCCGGA               T^CCGGA
AflII         418  CTTAAG               C^TTAAG
AflIII       2823  ACATGT               A^C[GA][CT]GT
AgeI          472  ACCGGT               A^CCGGT
Alw44I       1379  GTGCAC               G^TGCAC
ApaLI        1379  GTGCAC               G^TGCAC
Asp700I      5319  GAATTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI         1372  CCCGAG               C^[CT]CG[GA]G
AvrII        2342  CCTAGG               C^CTAGG
BamHI        2191  GGATCC               G^GATCC
BanI         2856  GGCACC               G^G[CT][GA]CC
BfrI          418  CTTAAG               C^TTAAG
BlnI         2342  CCTAGG               C^CTAGG
BseAI        2537  TCCGGA               T^CCGGA
BsgI         4161  CTGCAC               GTGCAG(16/14)
BsoBI        1372  CCCGAG               C^[CT]CG[GA]G
BspEI        2537  TCCGGA               T^CCGGA
BspHI        5113  TCATGA               T^CATGA
BspLU11I     2823  ACATGT               A^CATGT
BstEII       3318  GGTCACC              G^GT[ACGT]ACC
BstPI        3318  GGTCACC              G^GT[ACGT]ACC
BstUI        3616  CGCG                 CG^CG
DraIII       3422  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          307  GAAGAG               CTCTTC(1/4)
EcoO65I      3318  GGTCACC              G^GT[ACGT]ACC
EcoRV        1889  GATATC               GAT^ATC
FseI         2833  GGCCGGCC             GGCCGG^CC
Ksp632I       307  GAAGAG               CTCTTC(1/4)
MroI         2537  TCCGGA               T^CCGGA
MvnI         3616  CGCG                 CG^CG
NaeI         2834  GCCGGC               GCC^GGC
NgoMIV       2834  GCCGGC               G^CCGGC
NheI          410  GCTAGC               G^CTAGC
PinAI         472  ACCGGT               A^CCGGT
RcaI         5113  TCATGA               T^CATGA
SexAI        2698  ACCAGGT              A^CC[AT]GGT
SpeI         5125  ACTAGT               A^CTAGT
XmnI         5319  GAATTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AcyI         4213  GACGTC               G[GA]^CG[CT]C
             4522  GACGCC              
ApaI         2407  GGGCCC               GGGCC^C
             4630  GGGCCC              
AseI          851  ATTAAT               AT^TAAT
             5025  ATTAAT              
AsnI          851  ATTAAT               AT^TAAT
             5025  ATTAAT              
AspI         1956  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2098  GACTCTGTC           
BlpI         2519  GCTCAGC              GC^T[ACGT]AGC
             4435  GCTAAGC             
Bpu1102I     2519  GCTCAGC              GC^T[ACGT]AGC
             4435  GCTAAGC             
BsaHI        4213  GACGTC               G[GA]^CG[CT]C
             4522  GACGCC              
BsaI         4084  GAGACC               GGTCTC(1/5)
             4354  GAGACC              
BsmBI        2487  CGTCTC               CGTCTC(1/5)
             2166  GAGACG              
BsmI         3843  GAATGC               GAATGC(1/-1)
             2453  GCATTC              
BspMI        1695  ACCTGC               ACCTGC(4/8)
             4246  ACCTGC              
BsrDI        3373  CATTGC               GCAATG(2/0)
             2046  GCAATG              
BsrFI         472  ACCGGT               [GA]^CCGG[CT]
             2834  GCCGGC              
BstAPI       1690  GCAGCACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3598  GCAGCAGATGC         
CelII        2519  GCTCAGC              GC^T[ACGT]AGC
             4435  GCTAAGC             
Cfr10I        472  ACCGGT               [GA]^CCGG[CT]
             2834  GCCGGC              
DraI         4919  TTTAAA               TTT^AAA
             5100  TTTAAA              
EcoNI        2525  CCTCAGACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4010  CCTACCTTAGG         
EcoRI         910  GAATTC               G^AATTC
             1899  GAATTC              
HaeII        1297  AGCGCC               [GA]GCGC^[CT]
             1398  AGCGCC              
HgaI         3614  GACGC                GACGC(5/10)
             4522  GACGC               
Hin1I        4213  GACGTC               G[GA]^CG[CT]C
             4522  GACGCC              
HindIII      3748  AAGCTT               A^AGCTT
             5214  AAGCTT              
PflFI        1956  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2098  GACTCTGTC           
PpuMI        1976  GGGACCT              [GA]G^G[AT]CC[CT]
             4399  GGGTCCC             
PshAI        2312  GACTTGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3651  GACATTTGTC          
PshBI         851  ATTAAT               AT^TAAT
             5025  ATTAAT              
PvuII        2426  CAGCTG               CAG^CTG
             2442  CAGCTG              
SmlI          418  CTTAAG               C^T[CT][GA]AG
             4179  CTCAAG              
SphI         2620  GCATGC               GCATG^C
             4113  GCATGC              
SspI         2144  AATATT               AAT^ATT
             4943  AATATT              
Tth111I      1956  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2098  GACTCTGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       AocI        ApoI        AspHI       AvaII       
BalI        BanII       BbsI        BbvCI       BbvI        
BciVI       BclI        BcnI        BfaI        BglI        
BmyI        BpmI        BpuAI       BsaJI       BsaWI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    Bsp1407I    BsrGI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        Eco81I      EcoO109I    
EcoRII      EcoT14I     FauI        FbaI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HincII      HindII      HinfI       HpaI        HpaII       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MfeI        MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MunI        MvaI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PstI        RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SspBI       StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII