##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Aor51HI AscI Asp700I Asp718I
AspEI AviII BamHI BbrPI BciVI
BclI BglII BlpI Bpu1102I BsaWI
BseAI BsiWI BspEI BspHI BssHII
BstAPI BstEII BstPI CelII CpoI
DraIII Eam1105I Eco47III EcoNI EcoO65I
EcoRV FbaI FspI KpnI KspI
MluI MroI NdeI NotI NruI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI SexAI SfiI SgrAI SnaBI
Sse8387I SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Alw44I 1500 GTGCAC G^TGCAC
AocI 4392 CCTGAGG CC^T[ACGT]AGG
ApaI 193 GGGCCC GGGCC^C
ApaLI 1500 GTGCAC G^TGCAC
AvrII 189 CCTAGG C^CTAGG
BbeI 2879 GGCGCC GGCGC^C
BcgI 2839 CGATTCTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 189 CCTAGG C^CTAGG
BsaAI 5125 TACGTG [CT]AC^GT[GA]
BsiEI 141 CGGCCG CG[GA][CT]^CG
Bsp1407I 3570 TGTACA T^GTACA
BspDI 5176 ATCGAT AT^CGAT
BspMI 2987 ACCTGC ACCTGC(4/8)
BsrBI 12 CCGCTC CCGCTC(-3/-3)
BsrFI 3037 GCCGGC [GA]^CCGG[CT]
BsrGI 3570 TGTACA T^GTACA
BssSI 4069 CTCGTG CACGAG(-5/-1)
Bst1107I 2641 GTATAC GTA^TAC
BstBI 4856 TTCGAA TT^CGAA
BstZ17I 2641 GTATAC GTA^TAC
Bsu36I 4392 CCTGAGG CC^T[ACGT]AGG
Cfr10I 3037 GCCGGC [GA]^CCGG[CT]
ClaI 5176 ATCGAT AT^CGAT
DraI 3542 TTTAAA TTT^AAA
DrdI 1165 GACACAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 141 CGGCCG C^GGCCG
EclXI 141 CGGCCG C^GGCCG
Eco52I 141 CGGCCG C^GGCCG
Eco81I 4392 CCTGAGG CC^T[ACGT]AGG
EcoT22I 598 ATGCAT ATGCA^T
FseI 3036 GGCCGGCC GGCCGG^CC
HpaI 3674 GTTAAC GTT^AAC
KasI 2879 GGCGCC G^GCGCC
MfeI 1792 CAATTG C^AATTG
MunI 1792 CAATTG C^AATTG
NaeI 3037 GCCGGC GCC^GGC
NarI 2879 GGCGCC GG^CGCC
NgoMIV 3037 GCCGGC G^CCGGC
NheI 4425 GCTAGC G^CTAGC
NsiI 598 ATGCAT ATGCA^T
NspV 4856 TTCGAA TT^CGAA
PstI 1027 CTGCAG CTGCA^G
SapI 3088 GAAGAGC GCTCTTC(1/4)
ScaI 1527 AGTACT AGT^ACT
SfoI 2879 GGCGCC GGC^GCC
SfuI 4856 TTCGAA TT^CGAA
SmaI 554 CCCGGG CCC^GGG
SspBI 3570 TGTACA T^GTACA
SspI 2375 AATATT AAT^ATT
StuI 3298 AGGCCT AGG^CCT
XbaI 2363 TCTAGA T^CTAGA
XmaCI 554 CCCGGG C^CCGGG
XmaI 554 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 2641 GTATAC GT^[AC][GT]AC
4756 GTAGAC
AflII 2275 CTTAAG C^TTAAG
3095 CTTAAG
AlwNI 201 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
5498 CAGTCTCTG
AseI 5006 ATTAAT AT^TAAT
5089 ATTAAT
AsnI 5006 ATTAAT AT^TAAT
5089 ATTAAT
AspI 2757 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4602 GACTCTGTC
AvaI 554 CCCGGG C^[CT]CG[GA]G
4503 CTCGGG
BanII 193 GGGCCC G[GA]GC[CT]^C
818 GAGCCC
BfrI 2275 CTTAAG C^TTAAG
3095 CTTAAG
BsaBI 1441 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5907 GATATGGATC
BsgI 4574 CTGCAC GTGCAG(16/14)
2788 GTGCAG
BsoBI 554 CCCGGG C^[CT]CG[GA]G
4503 CTCGGG
BsrDI 5016 CATTGC GCAATG(2/0)
1193 GCAATG
BstXI 1012 CCAGATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3002 CCAAAGTGCTGG
DraII 192 AGGGCCC [GA]G^G[ACGT]CC[CT]
451 AGGGCCT
EcoO109I 192 AGGGCCC [GA]G^G[ACGT]CC[CT]
451 AGGGCCT
EcoRI 3666 GAATTC G^AATTC
5077 GAATTC
HaeII 1237 GGCGCT [GA]GCGC^[CT]
2879 GGCGCC
HgaI 1453 GACGC GACGC(5/10)
3888 GACGC
HincII 331 GTCAAC GT[CT]^[GA]AC
3674 GTTAAC
HindII 331 GTCAAC GT[CT]^[GA]AC
3674 GTTAAC
HindIII 1272 AAGCTT A^AGCTT
3779 AAGCTT
MamI 1441 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5907 GATATGGATC
PflFI 2757 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4602 GACTCTGTC
PshBI 5006 ATTAAT AT^TAAT
5089 ATTAAT
SphI 600 GCATGC GCATG^C
4480 GCATGC
Tth111I 2757 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4602 GACTCTGTC
XcmI 2951 CCAGGTTGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3785 CCAGGAGCCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI AspHI
AvaII BalI BanI BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI Bsp1286I BspLU11I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SpeI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII