##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AccII AccIII AclI AgeI
AhdI Aor51HI AscI AspEI AspI
AviII BbrPI BcgI BglI BseAI
BsgI BsiWI BsmBI BspEI BsrFI
BssHII BssSI Bst1107I BstAPI BstUI
BstZ17I Cfr10I CpoI DraIII DrdI
Eam1105I Eco47III EcoRV FseI FspI
KspI MfeI MluI MroI MunI
MvnI NaeI NgoMIV NotI NruI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SapI SbfI SexAI
SfiI SgrAI SnaBI SpeI Sse8387I
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 973 GGTACC G^GTACC
AcyI 2203 GGCGCC G[GA]^CG[CT]C
AflII 1926 CTTAAG C^TTAAG
ApaI 5090 GGGCCC GGGCC^C
Asp718I 973 GGTACC G^GTACC
BamHI 5692 GGATCC G^GATCC
BbeI 2203 GGCGCC GGCGC^C
BfrI 1926 CTTAAG C^TTAAG
BglII 5763 AGATCT A^GATCT
BsaAI 3660 CACGTA [CT]AC^GT[GA]
BsaHI 2203 GGCGCC G[GA]^CG[CT]C
BsaWI 308 TCCGGT [AT]^CCGG[AT]
BsiEI 31 CGGCCG CG[GA][CT]^CG
Bsp1407I 5478 TGTACA T^GTACA
BspDI 5155 ATCGAT AT^CGAT
BsrBI 2785 CCGCTC CCGCTC(-3/-3)
BsrDI 2113 GCAATG GCAATG(2/0)
BsrGI 5478 TGTACA T^GTACA
BstBI 1640 TTCGAA TT^CGAA
ClaI 5155 ATCGAT AT^CGAT
EagI 31 CGGCCG C^GGCCG
EclXI 31 CGGCCG C^GGCCG
Eco52I 31 CGGCCG C^GGCCG
Hin1I 2203 GGCGCC G[GA]^CG[CT]C
HpaI 2470 GTTAAC GTT^AAC
KasI 2203 GGCGCC G^GCGCC
KpnI 973 GGTACC GGTAC^C
NarI 2203 GGCGCC GG^CGCC
NdeI 4474 CATATG CA^TATG
NspV 1640 TTCGAA TT^CGAA
SacI 4956 GAGCTC GAGCT^C
SalI 436 GTCGAC G^TCGAC
SfoI 2203 GGCGCC GGC^GCC
SfuI 1640 TTCGAA TT^CGAA
SphI 1981 GCATGC GCATG^C
SspBI 5478 TGTACA T^GTACA
XbaI 2771 TCTAGA T^CTAGA
XcmI 3605 CCAGTGAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Alw44I 4257 GTGCAC G^TGCAC
4865 GTGCAC
AlwNI 501 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
564 CAGCGTCTG
ApaLI 4257 GTGCAC G^TGCAC
4865 GTGCAC
AseI 1342 ATTAAT AT^TAAT
1850 ATTAAT
AsnI 1342 ATTAAT AT^TAAT
1850 ATTAAT
AvaI 4649 CCCGGG C^[CT]CG[GA]G
5024 CCCGGG
AvrII 2100 CCTAGG C^CTAGG
4873 CCTAGG
BalI 1507 TGGCCA TGG^CCA
6219 TGGCCA
BclI 881 TGATCA T^GATCA
4379 TGATCA
BlnI 2100 CCTAGG C^CTAGG
4873 CCTAGG
BlpI 155 GCTGAGC GC^T[ACGT]AGC
5545 GCTGAGC
BpmI 1000 CTGGAG CTGGAG(16/14)
5759 CTGGAG
Bpu1102I 155 GCTGAGC GC^T[ACGT]AGC
5545 GCTGAGC
BsaBI 2758 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5729 GATCCAGATC
BseRI 1395 CTCCTC GAGGAG(10/8)
144 GAGGAG
BsoBI 4649 CCCGGG C^[CT]CG[GA]G
5024 CCCGGG
CelII 155 GCTGAGC GC^T[ACGT]AGC
5545 GCTGAGC
EcoNI 1019 CCTCAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1547 CCTTTGCCAGG
EcoRI 1403 GAATTC G^AATTC
1453 GAATTC
EcoT22I 1413 ATGCAT ATGCA^T
4695 ATGCAT
FauI 574 CCCGC CCCGC(4/6)
2305 CCCGC
FbaI 881 TGATCA T^GATCA
4379 TGATCA
HaeII 152 GGCGCT [GA]GCGC^[CT]
2203 GGCGCC
HgaI 206 GCGTC GACGC(5/10)
566 GCGTC
HincII 436 GTCGAC GT[CT]^[GA]AC
2470 GTTAAC
HindII 436 GTCGAC GT[CT]^[GA]AC
2470 GTTAAC
MamI 2758 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5729 GATCCAGATC
MluNI 1507 TGGCCA TGG^CCA
6219 TGGCCA
MscI 1507 TGGCCA TGG^CCA
6219 TGGCCA
NsiI 1413 ATGCAT ATGCA^T
4695 ATGCAT
PflMI 1910 CCACAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4127 CCATCAGCTGG
PshBI 1342 ATTAAT AT^TAAT
1850 ATTAAT
SmaI 4649 CCCGGG CCC^GGG
5024 CCCGGG
StuI 256 AGGCCT AGG^CCT
6128 AGGCCT
Van91I 1910 CCACAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4127 CCATCAGCTGG
XmaCI 4649 CCCGGG C^CCGGG
5024 CCCGGG
XmaI 4649 CCCGGG C^CCGGG
5024 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AocI ApoI Asp700I
AspHI AvaII BanI BanII BbsI
BbvCI BbvI BciVI BcnI BfaI
BmyI BpuAI BsaI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspHI BspLU11I BspMI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoO65I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NheI NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI