##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I AocI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BglI BlnI BsaAI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BspDI BspEI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstUI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoRV FseI FspI
HgaI Hin1I KasI KpnI KspI
MluI MroI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI Sse8387I Tth111I Van91I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 4043 GTAGAC GT^[AC][GT]AC
Aor51HI 382 AGCGCT AGC^GCT
ApaI 4371 GGGCCC GGGCC^C
AspHI 3510 GAGCTC G[AT]GC[AT]^C
AvaI 4389 CTCGAG C^[CT]CG[GA]G
BcgI 3214 GCATTTTATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 3613 CCGGG CC^[GC]GG
BsaBI 5489 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 3062 GAGGAG GAGGAG(10/8)
BsiHKAI 3510 GAGCTC G[AT]GC[AT]^C
BsoBI 4389 CTCGAG C^[CT]CG[GA]G
Bsp1407I 716 TGTACA T^GTACA
BspLU11I 5335 ACATGT A^CATGT
BspMI 189 ACCTGC ACCTGC(4/8)
BsrGI 716 TGTACA T^GTACA
BstEII 5802 GGTCACC G^GT[ACGT]ACC
BstPI 5802 GGTCACC G^GT[ACGT]ACC
Eco47III 382 AGCGCT AGC^GCT
EcoO65I 5802 GGTCACC G^GT[ACGT]ACC
EcoT22I 1027 ATGCAT ATGCA^T
HaeII 382 AGCGCT [GA]GCGC^[CT]
HapII 3613 CCGG C^CGG
HindIII 1 AAGCTT A^AGCTT
HpaI 5288 GTTAAC GTT^AAC
HpaII 3613 CCGG C^CGG
MamI 5489 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 3613 CCGG C^CGG
NciI 3613 CCGGG CC^[GC]GG
NdeI 2402 CATATG CA^TATG
NsiI 1027 ATGCAT ATGCA^T
PaeR7I 4389 CTCGAG C^TCGAG
SacI 3510 GAGCTC GAGCT^C
SpeI 4835 ACTAGT A^CTAGT
SphI 4926 GCATGC GCATG^C
SspBI 716 TGTACA T^GTACA
SwaI 3738 ATTTAAAT ATTT^AAAT
XcmI 1674 CCAAAATCAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4389 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 58 ACGTGT A^C[GA][CT]GT
5335 ACATGT
AlwNI 3627 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
3630 CAGCTGCTG
BalI 1182 TGGCCA TGG^CCA
4702 TGGCCA
BciVI 2368 GGATAC GTATCC(6/5)
4474 GGATAC
BspHI 195 TCATGA T^CATGA
2557 TCATGA
BsrDI 1957 CATTGC GCAATG(2/0)
2983 CATTGC
BstBI 2751 TTCGAA TT^CGAA
4134 TTCGAA
BstF5I 866 CATCC GGATG(2/0)
1687 GGATG
DraII 2337 AGGGCCT [GA]G^G[ACGT]CC[CT]
4370 AGGGCCC
EaeI 1182 TGGCCA [CT]^GGCC[GA]
4702 TGGCCA
EcoO109I 2337 AGGGCCT [GA]G^G[ACGT]CC[CT]
4370 AGGGCCC
EcoRI 4131 GAATTC G^AATTC
5929 GAATTC
EcoT14I 1410 CCAAGG C^C[AT][AT]GG
2196 CCTTGG
FauI 695 CCCGC CCCGC(4/6)
1573 GCGGG
FokI 866 CATCC GGATG(9/13)
1687 GGATG
HincII 2594 GTTGAC GT[CT]^[GA]AC
5288 GTTAAC
HindII 2594 GTTGAC GT[CT]^[GA]AC
5288 GTTAAC
MfeI 221 CAATTG C^AATTG
5711 CAATTG
MluNI 1182 TGGCCA TGG^CCA
4702 TGGCCA
MscI 1182 TGGCCA TGG^CCA
4702 TGGCCA
MunI 221 CAATTG C^AATTG
5711 CAATTG
NspV 2751 TTCGAA TT^CGAA
4134 TTCGAA
PvuII 3630 CAGCTG CAG^CTG
5408 CAGCTG
RcaI 195 TCATGA T^CATGA
2557 TCATGA
SapI 226 GAAGAGC GCTCTTC(1/4)
4382 GCTCTTC
SfuI 2751 TTCGAA TT^CGAA
4134 TTCGAA
StuI 2055 AGGCCT AGG^CCT
5365 AGGCCT
StyI 1410 CCAAGG C^C[AT][AT]GG
2196 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI ApoI AseI AsnI Asp700I
AvaII BanII BbsI BbvI BclI
BfaI BfrI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstNI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI EarI EcoRII FbaI Fnu4HI
HaeIII HhaI HinP1I HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PshBI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XhoII XmnI