##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccII AccIII AclI AgeI
AocI Aor51HI ApaI AscI AspI
AviII BamHI BbeI BbrPI BcgI
BcnI BglII BsaAI BsaWI BseAI
BsiEI BspDI BspEI BspMI BsrBI
BsrFI BssHII Bst1107I BstBI BstUI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraII DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoRV FauI FseI FspI HaeII
HapII HpaII KasI KspI MluI
MroI MspI MvnI NaeI NarI
NciI NdeI NgoMIV NotI NruI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
Tth111I Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 4868 GGTACC G^GTACC
AcyI 4802 GGCGTC G[GA]^CG[CT]C
AflIII 4518 ACATGT A^C[GA][CT]GT
AhdI 4797 GACACGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 5284 GTGCAC G^TGCAC
ApaLI 5284 GTGCAC G^TGCAC
Asp718I 4868 GGTACC G^GTACC
AspEI 4797 GACACGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 3896 CTCGAG C^[CT]CG[GA]G
AvrII 2229 CCTAGG C^CTAGG
BalI 4684 TGGCCA TGG^CCA
BbvCI 3010 GCTGAGG CCTCAGC(-5/-2)
BlnI 2229 CCTAGG C^CTAGG
BlpI 2833 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2833 GCTCAGC GC^T[ACGT]AGC
BsaHI 4802 GGCGTC G[GA]^CG[CT]C
BsiWI 1417 CGTACG C^GTACG
BsmBI 1808 GAGACG CGTCTC(1/5)
BsmFI 4263 GTCCC GGGAC(10/14)
BsoBI 3896 CTCGAG C^[CT]CG[GA]G
BspLU11I 4518 ACATGT A^CATGT
BssSI 1835 CTCGTG CACGAG(-5/-1)
BstEII 3868 GGTGACC G^GT[ACGT]ACC
BstPI 3868 GGTGACC G^GT[ACGT]ACC
CelII 2833 GCTCAGC GC^T[ACGT]AGC
DsaI 3035 CCATGG C^C[GA][CT]GG
EaeI 4684 TGGCCA [CT]^GGCC[GA]
Eam1105I 4797 GACACGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3868 GGTGACC G^GT[ACGT]ACC
Hin1I 4802 GGCGTC G[GA]^CG[CT]C
HpaI 5153 GTTAAC GTT^AAC
KpnI 4868 GGTACC GGTAC^C
MluNI 4684 TGGCCA TGG^CCA
MscI 4684 TGGCCA TGG^CCA
MspA1I 446 CAGCTG C[AC]G^C[GT]G
NcoI 3035 CCATGG C^CATGG
NheI 2451 GCTAGC G^CTAGC
PaeR7I 3896 CTCGAG C^TCGAG
PvuII 446 CAGCTG CAG^CTG
SapI 757 GAAGAGC GCTCTTC(1/4)
ScaI 903 AGTACT AGT^ACT
SphI 17 GCATGC GCATG^C
SwaI 3630 ATTTAAAT ATTT^AAAT
XbaI 5111 TCTAGA T^CTAGA
XhoI 3896 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 3816 CCGC CCGC(-3/-1)
4006 GCGG
AflII 2121 CTTAAG C^TTAAG
2704 CTTAAG
BanI 3779 GGTGCC G^G[CT][GA]CC
4868 GGTACC
BfrI 2121 CTTAAG C^TTAAG
2704 CTTAAG
BglI 1118 GCCTCATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1567 GCCCTTGAGGC
BsaBI 984 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5168 GATTTTAATC
BsaI 3884 GGTCTC GGTCTC(1/5)
4277 GGTCTC
Bsp1407I 1002 TGTACA T^GTACA
3998 TGTACA
BsrGI 1002 TGTACA T^GTACA
3998 TGTACA
CfoI 15 GCGC GCG^C
21 GCGC
EcoNI 5 CCTGGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1994 CCTGGCTTAGG
EcoRI 3221 GAATTC G^AATTC
5426 GAATTC
HgaI 1318 GCGTC GACGC(5/10)
4803 GCGTC
HhaI 15 GCGC GCG^C
21 GCGC
HinP1I 15 GCGC G^CGC
21 GCGC
MamI 984 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5168 GATTTTAATC
SspBI 1002 TGTACA T^GTACA
3998 TGTACA
SspI 2399 AATATT AAT^ATT
5628 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
AspHI AvaII BanII BbsI BbvI
BciVI BclI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmI Bsp1286I
BspHI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI EarI EcoRII
EcoT14I EcoT22I FbaI Fnu4HI FokI
HaeIII HincII HindII HindIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MfeI MflI
MnlI MseI MslI MunI MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI PleI PshBI PstI RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI