##################################
# NO CUT ENZYMES (   75 enzymes) #
##################################

Acc65I      AccIII      AgeI        AhdI        Aor51HI     
ApaI        AscI        Asp718I     AspEI       AvrII       
BamHI       BbeI        BcgI        BciVI       BlnI        
BsaWI       BseAI       BsiEI       BsiWI       BspDI       
BspEI       BspLU11I    BsrBI       BsrFI       BssHII      
BstBI       Cfr10I      ClaI        CpoI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      FseI        HaeII       HgaI        HpaI        
KasI        KpnI        KspI        MluI        MroI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PinAI       PmeI        
PshAI       PvuI        RsrII       SacI        SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SphI        
Sse8387I    StuI        SwaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AatII        2798  GACGTC               GACGT^C
AccII        4343  CGCG                 CG^CG
AclI         3574  AACGTT               AA^CGTT
AcyI         2798  GACGTC               G[GA]^CG[CT]C
Alw44I        950  GTGCAC               G^TGCAC
ApaLI         950  GTGCAC               G^TGCAC
AviII        4429  TGCGCA               TGC^GCA
BanI         1149  GGTGCC               G^G[CT][GA]CC
BcnI         4488  CCGGG                CC^[GC]GG
BglI         3341  GCCACAACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         1705  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     1705  GCTAAGC              GC^T[ACGT]AGC
BsaBI        2152  GATTTGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        2798  GACGTC               G[GA]^CG[CT]C
BseRI        6329  GAGGAG               GAGGAG(10/8)
BspMI        1987  ACCTGC               ACCTGC(4/8)
BssSI        4725  CACGAG               CACGAG(-5/-1)
Bst1107I     6511  GTATAC               GTA^TAC
BstEII        414  GGTCACC              G^GT[ACGT]ACC
BstPI         414  GGTCACC              G^GT[ACGT]ACC
BstUI        4343  CGCG                 CG^CG
BstZ17I      6511  GTATAC               GTA^TAC
CelII        1705  GCTAAGC              GC^T[ACGT]AGC
DsaI         3492  CCATGG               C^C[GA][CT]GG
EcoNI        1131  CCTTCAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       414  GGTCACC              G^GT[ACGT]ACC
EcoT22I      3998  ATGCAT               ATGCA^T
FspI         4429  TGCGCA               TGC^GCA
Hin1I        2798  GACGTC               G[GA]^CG[CT]C
MamI         2152  GATTTGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I       2252  CAGCTG               C[AC]G^C[GT]G
MvnI         4343  CGCG                 CG^CG
NciI         4488  CCGGG                CC^[GC]GG
NcoI         3492  CCATGG               C^CATGG
NdeI         3659  CATATG               CA^TATG
NsiI         3998  ATGCAT               ATGCA^T
NspI         4434  ACATGC               [GA]CATG^[CT]
PaeR7I       4813  CTCGAG               C^TCGAG
PflMI         971  CCAATCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I     3574  AACGTT               AA^CGTT
PvuII        2252  CAGCTG               CAG^CTG
SnaBI         202  TACGTA               TAC^GTA
Van91I        971  CCAATCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          419  CCATACATGGAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         4813  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccI         4938  GTCTAC               GT^[AC][GT]AC
             6511  GTATAC              
AflII        2871  CTTAAG               C^TTAAG
             3625  CTTAAG              
AflIII       3636  ACACGT               A^C[GA][CT]GT
             3638  ACGTGT              
Asp700I      1853  GAATAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2316  GAAACAGTTC          
AvaI         4405  CCCGAG               C^[CT]CG[GA]G
             4813  CTCGAG              
BalI         1439  TGGCCA               TGG^CCA
             4574  TGGCCA              
BbrPI        3637  CACGTG               CAC^GTG
             6129  CACGTG              
BclI         3913  TGATCA               T^GATCA
             5481  TGATCA              
BfrI         2871  CTTAAG               C^TTAAG
             3625  CTTAAG              
BsoBI        4405  CCCGAG               C^[CT]CG[GA]G
             4813  CTCGAG              
Bsp1407I     1656  TGTACA               T^GTACA
             5966  TGTACA              
BsrGI        1656  TGTACA               T^GTACA
             5966  TGTACA              
EcoRI        2019  GAATTC               G^AATTC
             5275  GAATTC              
EcoRV        1364  GATATC               GAT^ATC
             3784  GATATC              
FauI         1787  CCCGC                CCCGC(4/6)
             4523  CCCGC               
FbaI         3913  TGATCA               T^GATCA
             5481  TGATCA              
HapII        1678  CCGG                 C^CGG
             4488  CCGG                
HincII       2785  GTTGAC               GT[CT]^[GA]AC
             4012  GTTGAC              
HindII       2785  GTTGAC               GT[CT]^[GA]AC
             4012  GTTGAC              
HpaII        1678  CCGG                 C^CGG
             4488  CCGG                
MfeI         2073  CAATTG               C^AATTG
             3064  CAATTG              
MluNI        1439  TGGCCA               TGG^CCA
             4574  TGGCCA              
MscI         1439  TGGCCA               TGG^CCA
             4574  TGGCCA              
MspI         1678  CCGG                 C^CGG
             4488  CCGG                
MunI         2073  CAATTG               C^AATTG
             3064  CAATTG              
PmaCI        3637  CACGTG               CAC^GTG
             6129  CACGTG              
PmlI         3637  CACGTG               CAC^GTG
             6129  CACGTG              
PpuMI         789  GGGTCCT              [GA]G^G[AT]CC[CT]
             2339  AGGACCC             
SspBI        1656  TGTACA               T^GTACA
             5966  TGTACA              
XmnI         1853  GAATAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2316  GAAACAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  108 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       AocI        ApoI        AseI        AsnI        
AspHI       AspI        AvaII       BanII       BbsI        
BbvCI       BbvI        BfaI        BglII       BmyI        
BpmI        BpuAI       BsaAI       BsaI        BsaJI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsmI        Bsp1286I    BspHI       
BsrDI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstXI       BstYI       Bsu36I      Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DraII       EaeI        EarI        Eco81I      EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HhaI        HinP1I      HindIII     HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PflFI       PleI        PshBI       PstI        
RcaI        RsaI        Sau3AI      Sau96I      ScaI        
ScrFI       SfaNI       SfcI        SmlI        SpeI        
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     XbaI        XhoII