##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccIII AflIII AgeI AhdI
Aor51HI AscI AspEI AviII AvrII
BbrPI BclI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CelII
Cfr10I ClaI CpoI DraIII DrdI
Eam1105I Eco47III EcoO65I FbaI FseI
FspI KspI MamI MluI MroI
NaeI NgoMIV NheI NotI NruI
NspV PinAI PmaCI PmeI PmlI
PvuI RsrII SacI SacII SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 32 GGTACC G^GTACC
AclI 3998 AACGTT AA^CGTT
AcyI 1933 GGCGCC G[GA]^CG[CT]C
AflII 1258 CTTAAG C^TTAAG
Alw44I 433 GTGCAC G^TGCAC
ApaI 4844 GGGCCC GGGCC^C
ApaLI 433 GTGCAC G^TGCAC
AseI 514 ATTAAT AT^TAAT
AsnI 514 ATTAAT AT^TAAT
Asp718I 32 GGTACC G^GTACC
AspI 2052 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 125 CTCGAG C^[CT]CG[GA]G
BamHI 3908 GGATCC G^GATCC
BanII 4844 GGGCCC G[GA]GC[CT]^C
BbeI 1933 GGCGCC GGCGC^C
BcgI 747 GCATGTGTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4113 GGATAC GTATCC(6/5)
BfrI 1258 CTTAAG C^TTAAG
BsaHI 1933 GGCGCC G[GA]^CG[CT]C
BsiEI 1592 CGGCCG CG[GA][CT]^CG
BsiWI 665 CGTACG C^GTACG
BsoBI 125 CTCGAG C^[CT]CG[GA]G
BssSI 41 CACGAG CACGAG(-5/-1)
EagI 1592 CGGCCG C^GGCCG
EclXI 1592 CGGCCG C^GGCCG
Eco52I 1592 CGGCCG C^GGCCG
EcoNI 4663 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 1933 GGCGCC [GA]GCGC^[CT]
Hin1I 1933 GGCGCC G[GA]^CG[CT]C
HpaI 574 GTTAAC GTT^AAC
KasI 1933 GGCGCC G^GCGCC
KpnI 32 GGTACC GGTAC^C
MslI 4903 CACACCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1933 GGCGCC GG^CGCC
NcoI 3729 CCATGG C^CATGG
NdeI 3110 CATATG CA^TATG
PacI 2894 TTAATTAA TTAAT^TAA
PaeR7I 125 CTCGAG C^TCGAG
PflFI 2052 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4636 CCATATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 979 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 514 ATTAAT AT^TAAT
Psp1406I 3998 AACGTT AA^CGTT
PvuII 3945 CAGCTG CAG^CTG
SalI 651 GTCGAC G^TCGAC
SfoI 1933 GGCGCC GGC^GCC
SwaI 2459 ATTTAAAT ATTT^AAAT
TfiI 374 GAATC G^A[AT]TC
Tth111I 2052 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4636 CCATATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2862 CCAGCTTCCAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 125 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 1789 CGCG CG^CG
4395 CGCG
AlwNI 3344 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
3600 CAGGCTCTG
Asp700I 409 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3060 GAAAAAATTC
AspHI 433 GTGCAC G[AT]GC[AT]^C
2797 GAGCAC
BcnI 235 CCGGG CC^[GC]GG
1580 CCCGG
BsiHKAI 433 GTGCAC G[AT]GC[AT]^C
2797 GAGCAC
BstUI 1789 CGCG CG^CG
4395 CGCG
BstYI 3908 GGATCC [GA]^GATC[CT]
4462 GGATCT
DsaI 319 CCACGG C^C[GA][CT]GG
3729 CCATGG
HgaI 4813 GACGC GACGC(5/10)
3497 GCGTC
HindIII 1335 AAGCTT A^AGCTT
4562 AAGCTT
MaeII 772 ACGT A^CGT
3999 ACGT
MfeI 2870 CAATTG C^AATTG
3098 CAATTG
MflI 3908 GGATCC [GA]^GATC[CT]
4462 GGATCT
MspA1I 623 CAGCGG C[AC]G^C[GT]G
3945 CAGCTG
MunI 2870 CAATTG C^AATTG
3098 CAATTG
MvnI 1789 CGCG CG^CG
4395 CGCG
NciI 235 CCGGG CC^[GC]GG
1580 CCCGG
XhoII 3908 GGATCC [GA]^GATC[CT]
4462 GGATCT
XmnI 409 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3060 GAAAAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AocI ApoI AvaII BalI
BanI BbsI BbvCI BbvI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmBI BsmFI BsmI Bsp1286I BspHI
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstXI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI Eco81I EcoO109I
EcoRI EcoRII EcoRV EcoT14I EcoT22I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MluNI MnlI
MscI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV NsiI NspI
PleI PpuMI PstI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI