##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AscI AseI AsnI AvrII BbrPI
BfrI BlnI BseAI BsiWI BspEI
BspLU11I BssHII Bst1107I BstZ17I CpoI
DrdI EagI EclXI Eco52I EcoRV
EcoT22I FseI HpaI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NotI NruI NsiI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SalI ScaI
SfiI SgrAI SnaBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 17 enzymes) #
###################################
Acc65I 2206 GGTACC G^GTACC
AccI 1138 GTAGAC GT^[AC][GT]AC
AhdI 6082 GACCGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3100 GTGCAC G^TGCAC
ApaLI 3100 GTGCAC G^TGCAC
Asp718I 2206 GGTACC G^GTACC
AspEI 6082 GACCGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 1092 CGATGTGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 7507 GGATAC GTATCC(6/5)
DraI 394 TTTAAA TTT^AAA
Eam1105I 6082 GACCGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HindIII 2081 AAGCTT A^AGCTT
KpnI 2206 GGTACC GGTAC^C
NheI 402 GCTAGC G^CTAGC
SbfI 1357 CCTGCAGG CCTGCA^GG
SpeI 8875 ACTAGT A^CTAGT
Sse8387I 1357 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AflIII 7538 ACGTGT A^C[GA][CT]GT
8897 ACGTGT
AviII 2558 TGCGCA TGC^GCA
4298 TGCGCA
BbeI 2264 GGCGCC GGCGC^C
8158 GGCGCC
BclI 1277 TGATCA T^GATCA
6461 TGATCA
BglI 2878 GCCATCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4399 GCCCAAGTGGC
BsaAI 7378 TACGTG [CT]AC^GT[GA]
8896 TACGTG
BsaBI 8301 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
8805 GATGTTCATC
BsaWI 5360 ACCGGA [AT]^CCGG[AT]
7325 ACCGGA
BsiEI 6081 CGACCG CG[GA][CT]^CG
7323 CGACCG
BsmBI 7648 GAGACG CGTCTC(1/5)
8147 GAGACG
BstAPI 488 GCAGATTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1351 GCAGGACCTGC
BstBI 2077 TTCGAA TT^CGAA
3061 TTCGAA
BstEII 5382 GGTCACC G^GT[ACGT]ACC
9051 GGTCACC
BstPI 5382 GGTCACC G^GT[ACGT]ACC
9051 GGTCACC
DraIII 7534 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
8544 CACTGTGTG
EcoO65I 5382 GGTCACC G^GT[ACGT]ACC
9051 GGTCACC
FbaI 1277 TGATCA T^GATCA
6461 TGATCA
FspI 2558 TGCGCA TGC^GCA
4298 TGCGCA
KasI 2264 GGCGCC G^GCGCC
8158 GGCGCC
MamI 8301 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
8805 GATGTTCATC
NarI 2264 GGCGCC GG^CGCC
8158 GGCGCC
NspV 2077 TTCGAA TT^CGAA
3061 TTCGAA
PshAI 114 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5766 GACCGCAGTC
SapI 7388 GAAGAGC GCTCTTC(1/4)
8100 GCTCTTC
SfoI 2264 GGCGCC GGC^GCC
8158 GGCGCC
SfuI 2077 TTCGAA TT^CGAA
3061 TTCGAA
SmaI 1103 CCCGGG CCC^GGG
3597 CCCGGG
SphI 1098 GCATGC GCATG^C
7301 GCATGC
SspI 5557 AATATT AAT^ATT
8199 AATATT
XbaI 2584 TCTAGA T^CTAGA
6282 TCTAGA
XmaCI 1103 CCCGGG C^CCGGG
3597 CCCGGG
XmaI 1103 CCCGGG C^CCGGG
3597 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 154 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI Aor51HI
ApaI ApoI Asp700I AspHI AspI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssKI
BssSI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I ClaI DdeI DpnII
DraII DsaI EaeI EarI Eco47III
Eco81I EcoNI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RcaI RsaI SacI
SacII Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmnI