##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
Alw44I Aor51HI ApaLI AscI Asp718I
AvaI AviII BamHI BbeI BbrPI
BbvCI BcgI BglI BsaAI BseAI
BsiWI BsoBI BspDI BspEI BsrBI
BsrFI BssHII BstBI BstEII BstPI
BstUI Cfr10I ClaI CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoO65I FseI FspI KasI KpnI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I StuI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 5136 GACGTC GACGT^C
AcyI 5136 GACGTC G[GA]^CG[CT]C
AflII 5730 CTTAAG C^TTAAG
AhdI 5934 GACAAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 3996 GGGCCC GGGCC^C
AspEI 5934 GACAAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2531 GAGCTC G[AT]GC[AT]^C
BcnI 334 CCCGG CC^[GC]GG
BfrI 5730 CTTAAG C^TTAAG
BsaHI 5136 GACGTC G[GA]^CG[CT]C
BsaWI 6366 TCCGGT [AT]^CCGG[AT]
BsiEI 966 CGGTCG CG[GA][CT]^CG
BsiHKAI 2531 GAGCTC G[AT]GC[AT]^C
BsmBI 5099 CGTCTC CGTCTC(1/5)
Bst1107I 3794 GTATAC GTA^TAC
BstAPI 6055 GCAGTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3794 GTATAC GTA^TAC
CfoI 5435 GCGC GCG^C
Eam1105I 5934 GACAAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 126 GATATC GAT^ATC
EcoT22I 2260 ATGCAT ATGCA^T
HaeII 5434 AGCGCC [GA]GCGC^[CT]
HgaI 3950 GACGC GACGC(5/10)
HhaI 5435 GCGC GCG^C
Hin1I 5136 GACGTC G[GA]^CG[CT]C
HinP1I 5435 GCGC G^CGC
HpaI 2579 GTTAAC GTT^AAC
NciI 334 CCCGG CC^[GC]GG
NdeI 1097 CATATG CA^TATG
NsiI 2260 ATGCAT ATGCA^T
SacI 2531 GAGCTC GAGCT^C
SalI 5476 GTCGAC G^TCGAC
SapI 1620 GAAGAGC GCTCTTC(1/4)
ScaI 936 AGTACT AGT^ACT
SpeI 3678 ACTAGT A^CTAGT
SphI 565 GCATGC GCATG^C
XcmI 4874 CCATTTATCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 3794 GTATAC GT^[AC][GT]AC
5476 GTCGAC
AlwNI 51 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
6056 CAGTGTCTG
AseI 4233 ATTAAT AT^TAAT
5382 ATTAAT
AsnI 4233 ATTAAT AT^TAAT
5382 ATTAAT
AspI 1411 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4550 GACTCAGTC
BciVI 1196 GTATCC GTATCC(6/5)
4197 GTATCC
BclI 2975 TGATCA T^GATCA
3944 TGATCA
BlpI 2123 GCTAAGC GC^T[ACGT]AGC
5237 GCTGAGC
Bpu1102I 2123 GCTAAGC GC^T[ACGT]AGC
5237 GCTGAGC
BsaBI 1225 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5527 GATTCAGATC
BsaI 1692 GAGACC GGTCTC(1/5)
3731 GGTCTC
BsmI 1286 GAATGC GAATGC(1/-1)
6122 GAATGC
Bsp1407I 4323 TGTACA T^GTACA
6012 TGTACA
BspMI 2151 GCAGGT ACCTGC(4/8)
4954 GCAGGT
BsrGI 4323 TGTACA T^GTACA
6012 TGTACA
BssSI 3889 CACGAG CACGAG(-5/-1)
2600 CTCGTG
CelII 2123 GCTAAGC GC^T[ACGT]AGC
5237 GCTGAGC
FauI 1179 CCCGC CCCGC(4/6)
5587 GCGGG
FbaI 2975 TGATCA T^GATCA
3944 TGATCA
HapII 335 CCGG C^CGG
6367 CCGG
HpaII 335 CCGG C^CGG
6367 CCGG
MamI 1225 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5527 GATTCAGATC
MspI 335 CCGG C^CGG
6367 CCGG
NcoI 1279 CCATGG C^CATGG
1516 CCATGG
PflFI 1411 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4550 GACTCAGTC
PshBI 4233 ATTAAT AT^TAAT
5382 ATTAAT
SmlI 3829 CTCAAG C^T[CT][GA]AG
5730 CTTAAG
SspBI 4323 TGTACA T^GTACA
6012 TGTACA
SspI 4284 AATATT AAT^ATT
6427 AATATT
Tth111I 1411 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4550 GACTCAGTC
XbaI 1119 TCTAGA T^CTAGA
5274 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AocI ApoI Asp700I AvaII
AvrII BalI BanI BanII BbsI
BbvI BfaI BglII BlnI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspHI BspLU11I BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HincII HindII
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI