##################################
# NO CUT ENZYMES ( 44 enzymes) #
##################################
AccIII AgeI AscI AviII BcgI
BglI BlpI Bpu1102I BseAI BsiWI
BspDI BspEI Bst1107I BstEII BstPI
BstZ17I CelII ClaI CpoI DrdI
EcoO65I EcoT22I FseI FspI HindIII
HpaI MfeI MroI MunI NotI
NruI NsiI PacI PaeR7I PinAI
PmeI PvuI RsrII SfiI SgrAI
SnaBI SpeI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 5977 GACGTC GACGT^C
AclI 58 AACGTT AA^CGTT
AhdI 388 GACCTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1160 CCTTAGG CC^T[ACGT]AGG
AspEI 388 GACCTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 580 CCTAGG C^CTAGG
BbeI 6142 GGCGCC GGCGC^C
BbrPI 5410 CACGTG CAC^GTG
BciVI 1478 GTATCC GTATCC(6/5)
BclI 2169 TGATCA T^GATCA
BlnI 580 CCTAGG C^CTAGG
BsaBI 1271 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 6285 ACCGGA [AT]^CCGG[AT]
BsiEI 3551 CGGCCG CG[GA][CT]^CG
BsmBI 3134 GAGACG CGTCTC(1/5)
BssHII 3012 GCGCGC G^CGCGC
BssSI 1367 CACGAG CACGAG(-5/-1)
BstBI 2834 TTCGAA TT^CGAA
Bsu36I 1160 CCTTAGG CC^T[ACGT]AGG
EagI 3551 CGGCCG C^GGCCG
Eam1105I 388 GACCTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3551 CGGCCG C^GGCCG
Eco52I 3551 CGGCCG C^GGCCG
Eco81I 1160 CCTTAGG CC^T[ACGT]AGG
EcoNI 3093 CCTGAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 4935 GATATC GAT^ATC
FbaI 2169 TGATCA T^GATCA
KasI 6142 GGCGCC G^GCGCC
KspI 3028 CCGCGG CCGC^GG
MamI 1271 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 5672 ACGCGT A^CGCGT
NaeI 80 GCCGGC GCC^GGC
NarI 6142 GGCGCC GG^CGCC
NdeI 1769 CATATG CA^TATG
NgoMIV 80 GCCGGC G^CCGGC
NheI 6002 GCTAGC G^CTAGC
NspV 2834 TTCGAA TT^CGAA
PmaCI 5410 CACGTG CAC^GTG
PmlI 5410 CACGTG CAC^GTG
PshAI 3591 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 58 AACGTT AA^CGTT
SacI 5910 GAGCTC GAGCT^C
SacII 3028 CCGCGG CCGC^GG
SalI 3640 GTCGAC G^TCGAC
SapI 1902 GAAGAGC GCTCTTC(1/4)
SbfI 6208 CCTGCAGG CCTGCA^GG
SfoI 6142 GGCGCC GGC^GCC
SfuI 2834 TTCGAA TT^CGAA
SmaI 3021 CCCGGG CCC^GGG
Sse8387I 6208 CCTGCAGG CCTGCA^GG
StuI 4056 AGGCCT AGG^CCT
XmaCI 3021 CCCGGG C^CCGGG
XmaI 3021 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 5637 GGTACC G^GTACC
5930 GGTACC
AflII 2083 CTTAAG C^TTAAG
5212 CTTAAG
Alw44I 4503 GTGCAC G^TGCAC
5618 GTGCAC
Aor51HI 1763 AGCGCT AGC^GCT
4185 AGCGCT
ApaLI 4503 GTGCAC G^TGCAC
5618 GTGCAC
Asp718I 5637 GGTACC G^GTACC
5930 GGTACC
BbvCI 5102 CCTCAGC CCTCAGC(-5/-2)
3472 GCTGAGG
BfrI 2083 CTTAAG C^TTAAG
5212 CTTAAG
BsaAI 2798 TACGTG [CT]AC^GT[GA]
5410 CACGTG
BsmI 1617 GAATGC GAATGC(1/-1)
1790 GCATTC
Bsp1407I 4127 TGTACA T^GTACA
6855 TGTACA
BspHI 4660 TCATGA T^CATGA
5241 TCATGA
BspLU11I 4119 ACATGT A^CATGT
5629 ACATGT
BsrDI 3623 CATTGC GCAATG(2/0)
5969 CATTGC
BsrGI 4127 TGTACA T^GTACA
6855 TGTACA
DraIII 6434 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
6504 CACCCTGTG
Eco47III 1763 AGCGCT AGC^GCT
4185 AGCGCT
EcoRI 2499 GAATTC G^AATTC
4583 GAATTC
KpnI 5637 GGTACC GGTAC^C
5930 GGTACC
NcoI 465 CCATGG C^CATGG
2607 CCATGG
RcaI 4660 TCATGA T^CATGA
5241 TCATGA
ScaI 5746 AGTACT AGT^ACT
5988 AGTACT
SphI 2744 GCATGC GCATG^C
6116 GCATGC
SspBI 4127 TGTACA T^GTACA
6855 TGTACA
SspI 240 AATATT AAT^ATT
3978 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApaI ApoI AseI AsnI Asp700I
AspHI AspI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XbaI XcmI XhoII
XmnI