##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccI AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AspEI AspI AviII BbrPI BseAI
BsiEI BsiWI BspEI BspHI BssHII
BssSI Bst1107I BstBI BstZ17I CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I FseI FspI KspI
MluI MroI NaeI NgoMIV NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 3074 GGTACC G^GTACC
AflII 3147 CTTAAG C^TTAAG
AflIII 5584 ACATGT A^C[GA][CT]GT
Asp718I 3074 GGTACC G^GTACC
BalI 1091 TGGCCA TGG^CCA
BamHI 4230 GGATCC G^GATCC
BbvCI 5376 CCTCAGC CCTCAGC(-5/-2)
BcgI 5365 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3147 CTTAAG C^TTAAG
BsaAI 6100 TACGTG [CT]AC^GT[GA]
BsaBI 3230 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1386 ACCGGA [AT]^CCGG[AT]
BspLU11I 5584 ACATGT A^CATGT
BsrBI 2814 GAGCGG CCGCTC(-3/-3)
BsrDI 61 GCAATG GCAATG(2/0)
BsrFI 355 ACCGGC [GA]^CCGG[CT]
BstAPI 4760 GCATGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 355 ACCGGC [GA]^CCGG[CT]
EaeI 1091 TGGCCA [CT]^GGCC[GA]
EcoRI 240 GAATTC G^AATTC
EcoRV 1264 GATATC GAT^ATC
EcoT22I 729 ATGCAT ATGCA^T
KpnI 3074 GGTACC GGTAC^C
MaeII 6101 ACGT A^CGT
MamI 3230 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1735 CAATTG C^AATTG
MluNI 1091 TGGCCA TGG^CCA
MscI 1091 TGGCCA TGG^CCA
MunI 1735 CAATTG C^AATTG
NheI 5065 GCTAGC G^CTAGC
NsiI 729 ATGCAT ATGCA^T
PaeR7I 4032 CTCGAG C^TCGAG
XbaI 4316 TCTAGA T^CTAGA
XhoI 4032 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 29 CGCG CG^CG
108 CGCG
AseI 5040 ATTAAT AT^TAAT
5950 ATTAAT
AsnI 5040 ATTAAT AT^TAAT
5950 ATTAAT
AvrII 1665 CCTAGG C^CTAGG
4497 CCTAGG
BbeI 95 GGCGCC GGCGC^C
3748 GGCGCC
BclI 4251 TGATCA T^GATCA
4969 TGATCA
BlnI 1665 CCTAGG C^CTAGG
4497 CCTAGG
BsmBI 6410 CGTCTC CGTCTC(1/5)
3081 GAGACG
Bsp1407I 1702 TGTACA T^GTACA
3115 TGTACA
BspDI 3267 ATCGAT AT^CGAT
3843 ATCGAT
BsrGI 1702 TGTACA T^GTACA
3115 TGTACA
BstUI 29 CGCG CG^CG
108 CGCG
ClaI 3267 ATCGAT AT^CGAT
3843 ATCGAT
FbaI 4251 TGATCA T^GATCA
4969 TGATCA
HaeII 95 GGCGCC [GA]GCGC^[CT]
3748 GGCGCC
HgaI 3083 GACGC GACGC(5/10)
3803 GCGTC
HpaI 2147 GTTAAC GTT^AAC
4922 GTTAAC
KasI 95 GGCGCC G^GCGCC
3748 GGCGCC
MvnI 29 CGCG CG^CG
108 CGCG
NarI 95 GGCGCC GG^CGCC
3748 GGCGCC
PflMI 3850 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4057 CCATCTGTTGG
PshBI 5040 ATTAAT AT^TAAT
5950 ATTAAT
SacI 606 GAGCTC GAGCT^C
4023 GAGCTC
SapI 947 GAAGAGC GCTCTTC(1/4)
4188 GCTCTTC
SexAI 5636 ACCAGGT A^CC[AT]GGT
6313 ACCAGGT
SfoI 95 GGCGCC GGC^GCC
3748 GGCGCC
SspBI 1702 TGTACA T^GTACA
3115 TGTACA
SspI 648 AATATT AAT^ATT
6559 AATATT
Van91I 3850 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4057 CCATCTGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
Asp700I AspHI AvaI AvaII BanI
BanII BbsI BbvI BciVI BcnI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrI BssKI BstEII
BstF5I BstNI BstPI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeI NdeII NlaIII
NlaIV NspI PleI PpuMI PshAI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmaCI XmaI
XmnI