##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AclI AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I BbeI BbrPI BglI BlpI
Bpu1102I BsaAI BsiWI BsmBI BsrFI
BssSI BstBI BstEII BstPI CelII
Cfr10I CpoI EagI EclXI Eco47III
Eco52I EcoO65I EcoRV FseI KasI
KpnI KspI MluI NaeI NarI
NgoMIV NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I RsrII SacII SalI SbfI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccII 3445 CGCG CG^CG
AcyI 5919 GGCGTC G[GA]^CG[CT]C
AflII 6270 CTTAAG C^TTAAG
AflIII 6432 ACATGT A^C[GA][CT]GT
AhdI 3319 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3319 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5706 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4333 CCTAGG C^CTAGG
BamHI 2637 GGATCC G^GATCC
BcgI 3908 GCAGCCGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2784 TGATCA T^GATCA
BfrI 6270 CTTAAG C^TTAAG
BlnI 4333 CCTAGG C^CTAGG
BsaHI 5919 GGCGTC G[GA]^CG[CT]C
BsiEI 2553 CGATCG CG[GA][CT]^CG
Bsp1407I 4355 TGTACA T^GTACA
BspDI 5904 ATCGAT AT^CGAT
BspLU11I 6432 ACATGT A^CATGT
BsrBI 4137 CCGCTC CCGCTC(-3/-3)
BsrGI 4355 TGTACA T^GTACA
BssHII 3444 GCGCGC G^CGCGC
Bst1107I 2457 GTATAC GTA^TAC
BstUI 3445 CGCG CG^CG
BstZ17I 2457 GTATAC GTA^TAC
ClaI 5904 ATCGAT AT^CGAT
DraIII 3802 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 5304 GACTGTTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3319 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 5848 GAATTC G^AATTC
FbaI 2784 TGATCA T^GATCA
Hin1I 5919 GGCGTC G[GA]^CG[CT]C
HindIII 4849 AAGCTT A^AGCTT
HpaI 96 GTTAAC GTT^AAC
MvnI 3445 CGCG CG^CG
PaeR7I 3305 CTCGAG C^TCGAG
PflFI 5706 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 758 GACACTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 2553 CGATCG CGAT^CG
SacI 3936 GAGCTC GAGCT^C
SapI 1416 GCTCTTC GCTCTTC(1/4)
ScaI 6266 AGTACT AGT^ACT
SexAI 2186 ACCAGGT A^CC[AT]GGT
SspBI 4355 TGTACA T^GTACA
Tth111I 5706 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2529 TCTAGA T^CTAGA
XhoI 3305 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 2457 GTATAC GT^[AC][GT]AC
2686 GTAGAC
AccIII 3992 TCCGGA T^CCGGA
4517 TCCGGA
ApaI 4247 GGGCCC GGGCC^C
4933 GGGCCC
Asp700I 2747 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3967 GAAGACATTC
AviII 3462 TGCGCA TGC^GCA
5972 TGCGCA
BalI 971 TGGCCA TGG^CCA
4956 TGGCCA
BanI 3418 GGTGCC G^G[CT][GA]CC
3814 GGTGCC
BbvCI 5350 CCTCAGC CCTCAGC(-5/-2)
1585 GCTGAGG
BsaBI 690 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1633 GATTTTGATC
BsaI 2433 GAGACC GGTCTC(1/5)
4287 GAGACC
BsaWI 3992 TCCGGA [AT]^CCGG[AT]
4517 TCCGGA
BseAI 3992 TCCGGA T^CCGGA
4517 TCCGGA
BspEI 3992 TCCGGA T^CCGGA
4517 TCCGGA
BsrDI 3614 CATTGC GCAATG(2/0)
6361 CATTGC
FspI 3462 TGCGCA TGC^GCA
5972 TGCGCA
HaeII 2382 GGCGCT [GA]GCGC^[CT]
5288 AGCGCC
HgaI 3325 GCGTC GACGC(5/10)
5920 GCGTC
HincII 96 GTTAAC GT[CT]^[GA]AC
1594 GTTGAC
HindII 96 GTTAAC GT[CT]^[GA]AC
1594 GTTGAC
MamI 690 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1633 GATTTTGATC
MfeI 818 CAATTG C^AATTG
1094 CAATTG
MluNI 971 TGGCCA TGG^CCA
4956 TGGCCA
MroI 3992 TCCGGA T^CCGGA
4517 TCCGGA
MscI 971 TGGCCA TGG^CCA
4956 TGGCCA
MunI 818 CAATTG C^AATTG
1094 CAATTG
NdeI 2159 CATATG CA^TATG
3860 CATATG
NheI 2385 GCTAGC G^CTAGC
5807 GCTAGC
SmaI 3422 CCCGGG CCC^GGG
4385 CCCGGG
XmaCI 3422 CCCGGG C^CCGGG
4385 CCCGGG
XmaI 3422 CCCGGG C^CCGGG
4385 CCCGGG
XmnI 2747 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3967 GAAGACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaI
AvaII BanII BbsI BbvI BciVI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII