##################################
# NO CUT ENZYMES (   53 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AgeI        
AhdI        AscI        Asp718I     AspEI       AviII       
AvrII       BamHI       BlnI        BlpI        Bpu1102I    
BseAI       BsiWI       BspDI       BspEI       BspMI       
BssHII      BssSI       BstAPI      BstEII      BstPI       
CelII       ClaI        DraIII      Eam1105I    EcoNI       
EcoO65I     FseI        FspI        KpnI        MluI        
MroI        NheI        NotI        NruI        PacI        
PaeR7I      PinAI       PmeI        PshAI       Psp1406I    
PvuI        SbfI        SexAI       SgrAI       SnaBI       
SphI        Sse8387I    XhoI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AflII        5730  CTTAAG               C^TTAAG
Aor51HI      2859  AGCGCT               AGC^GCT
BalI         6128  TGGCCA               TGG^CCA
BbrPI        1156  CACGTG               CAC^GTG
BbsI          722  GAAGAC               GAAGAC(2/6)
BciVI        1324  GGATAC               GTATCC(6/5)
BfrI         5730  CTTAAG               C^TTAAG
BpuAI         722  GAAGAC               GAAGAC(2/6)
BsaAI        1156  CACGTG               [CT]AC^GT[GA]
BsaBI         938  GATAGGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1213  ACCGGA               [AT]^CCGG[AT]
BsmI         5355  GAATGC               GAATGC(1/-1)
Bsp1407I      948  TGTACA               T^GTACA
BspLU11I     4302  ACATGT               A^CATGT
BsrBI         224  CCGCTC               CCGCTC(-3/-3)
BsrGI         948  TGTACA               T^GTACA
Bst1107I     1801  GTATAC               GTA^TAC
BstZ17I      1801  GTATAC               GTA^TAC
DrdI         4581  GACGGCCAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     2859  AGCGCT               AGC^GCT
EcoRI        1384  GAATTC               G^AATTC
EcoRV        5167  GATATC               GAT^ATC
EcoT22I      5782  ATGCAT               ATGCA^T
HgaI          206  GACGC                GACGC(5/10)
KspI          103  CCGCGG               CCGC^GG
MamI          938  GATAGGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        6128  TGGCCA               TGG^CCA
MscI         6128  TGGCCA               TGG^CCA
NsiI         5782  ATGCAT               ATGCA^T
PflMI        2950  CCATCTCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1156  CACGTG               CAC^GTG
PmlI         1156  CACGTG               CAC^GTG
PpuMI         409  GGGACCT              [GA]G^G[AT]CC[CT]
SacII         103  CCGCGG               CCGC^GG
SalI         4590  GTCGAC               G^TCGAC
SapI         4457  GAAGAGC              GCTCTTC(1/4)
ScaI         5103  AGTACT               AGT^ACT
SpeI         3389  ACTAGT               A^CTAGT
SspBI         948  TGTACA               T^GTACA
StuI           54  AGGCCT               AGG^CCT
Van91I       2950  CCATCTCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         2332  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   41 enzymes) #
###################################

AflIII       1157  ACGTGT               A^C[GA][CT]GT
             4302  ACATGT              
Alw44I       4908  GTGCAC               G^TGCAC
             5464  GTGCAC              
AocI         1229  CCTGAGG              CC^T[ACGT]AGG
             6192  CCTGAGG             
ApaLI        4908  GTGCAC               G^TGCAC
             5464  GTGCAC              
AseI         2375  ATTAAT               AT^TAAT
             5159  ATTAAT              
AsnI         2375  ATTAAT               AT^TAAT
             5159  ATTAAT              
Asp700I      1043  GAAGTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5005  GAATGGATTC          
AspI          206  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             6399  GACTCCGTC           
BanI          116  GGCGCC               G^G[CT][GA]CC
              488  GGCGCC              
BbeI          116  GGCGCC               GGCGC^C
              488  GGCGCC              
BbvCI         160  GCTGAGG              CCTCAGC(-5/-2)
             3878  GCTGAGG             
BglII        2254  AGATCT               A^GATCT
             2613  AGATCT              
BsgI         6371  CTGCAC               GTGCAG(16/14)
              770  GTGCAG              
BstBI        2997  TTCGAA               TT^CGAA
             3756  TTCGAA              
BstYI        2254  AGATCT               [GA]^GATC[CT]
             2613  AGATCT              
Bsu36I       1229  CCTGAGG              CC^T[ACGT]AGG
             6192  CCTGAGG             
CpoI           94  CGGTCCG              CG^G[AT]CCG
              172  CGGACCG             
Eco81I       1229  CCTGAGG              CC^T[ACGT]AGG
             6192  CCTGAGG             
HindIII       815  AAGCTT               A^AGCTT
             1656  AAGCTT              
HpaI         2051  GTTAAC               GTT^AAC
             2903  GTTAAC              
KasI          116  GGCGCC               G^GCGCC
              488  GGCGCC              
MfeI         3243  CAATTG               C^AATTG
             3354  CAATTG              
MflI         2254  AGATCT               [GA]^GATC[CT]
             2613  AGATCT              
MunI         3243  CAATTG               C^AATTG
             3354  CAATTG              
NarI          116  GGCGCC               GG^CGCC
              488  GGCGCC              
NdeI         3653  CATATG               CA^TATG
             4364  CATATG              
NspV         2997  TTCGAA               TT^CGAA
             3756  TTCGAA              
PflFI         206  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             6399  GACTCCGTC           
PshBI        2375  ATTAAT               AT^TAAT
             5159  ATTAAT              
RsrII          94  CGGTCCG              CG^G[AT]CCG
              172  CGGACCG             
SacI         2783  GAGCTC               GAGCT^C
             3035  GAGCTC              
SfiI           40  GGCCGTAGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
              267  GGCCGGGCGGGCC       
SfoI          116  GGCGCC               GGC^GCC
              488  GGCGCC              
SfuI         2997  TTCGAA               TT^CGAA
             3756  TTCGAA              
SmaI           72  CCCGGG               CCC^GGG
              349  CCCGGG              
SwaI         5795  ATTTAAAT             ATTT^AAAT
             6112  ATTTAAAT            
Tth111I       206  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             6399  GACTCCGTC           
XhoII        2254  AGATCT               [GA]^GATC[CT]
             2613  AGATCT              
XmaCI          72  CCCGGG               C^CCGGG
              349  CCCGGG              
XmaI           72  CCCGGG               C^CCGGG
              349  CCCGGG              
XmnI         1043  GAAGTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5005  GAATGGATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  122 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AluI        AlwI        AlwNI       ApaI        
ApoI        AspHI       AvaI        AvaII       BanII       
BbvI        BcgI        BclI        BcnI        BfaI        
BglI        BmyI        BpmI        BsaHI       BsaI        
BsaJI       BseRI       BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    BspHI       BsrDI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
EagI        EarI        EclXI       Eco52I      EcoO109I    
EcoRII      EcoT14I     FauI        FbaI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NciI        NcoI        
NdeII       NgoMIV      NlaIII      NlaIV       NspI        
PleI        PstI        PvuII       RcaI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XcmI