##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
Alw44I ApaLI AscI Asp700I AvrII
BamHI BbeI BbvCI BciVI BglI
BlnI BsaHI BseAI BsiEI BsiWI
BspDI BspEI BspLU11I BstBI ClaI
CpoI DrdI EagI EclXI Eco52I
EcoRI EcoRV FseI Hin1I HpaI
KasI KspI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PflMI PinAI PmeI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI Van91I
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 6015 GGTACC G^GTACC
AflII 6125 CTTAAG C^TTAAG
AhdI 1908 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2528 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 488 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2884 AGCGCT AGC^GCT
ApaI 236 GGGCCC GGGCC^C
Asp718I 6015 GGTACC G^GTACC
AspEI 1908 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1984 TGCGCA TGC^GCA
BalI 2755 TGGCCA TGG^CCA
BbrPI 2542 CACGTG CAC^GTG
BcgI 1714 GCAACGTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 6125 CTTAAG C^TTAAG
BsaWI 1241 ACCGGA [AT]^CCGG[AT]
BsrFI 1952 GCCGGT [GA]^CCGG[CT]
BssHII 2162 GCGCGC G^CGCGC
Bst1107I 3286 GTATAC GTA^TAC
BstAPI 1990 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1300 GGTTACC G^GT[ACGT]ACC
BstPI 1300 GGTTACC G^GT[ACGT]ACC
BstZ17I 3286 GTATAC GTA^TAC
Bsu36I 488 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1952 GCCGGT [GA]^CCGG[CT]
Eam1105I 1908 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2884 AGCGCT AGC^GCT
Eco81I 488 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1300 GGTTACC G^GT[ACGT]ACC
EcoT22I 3315 ATGCAT ATGCA^T
FspI 1984 TGCGCA TGC^GCA
HindIII 1628 AAGCTT A^AGCTT
KpnI 6015 GGTACC GGTAC^C
MluNI 2755 TGGCCA TGG^CCA
MscI 2755 TGGCCA TGG^CCA
NsiI 3315 ATGCAT ATGCA^T
PmaCI 2542 CACGTG CAC^GTG
PmlI 2542 CACGTG CAC^GTG
PshAI 1673 GACCGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1806 GAAGAGC GCTCTTC(1/4)
SphI 3317 GCATGC GCATG^C
SspI 1463 AATATT AAT^ATT
StuI 2137 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 1717 ACGTGT A^C[GA][CT]GT
3515 ACGTGT
AseI 1284 ATTAAT AT^TAAT
1596 ATTAAT
AsnI 1284 ATTAAT AT^TAAT
1596 ATTAAT
BanI 2269 GGCACC G^G[CT][GA]CC
6015 GGTACC
BclI 757 TGATCA T^GATCA
1559 TGATCA
BglII 1005 AGATCT A^GATCT
1118 AGATCT
BsaBI 3990 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4174 GATCCCCATC
BsaI 1429 GAGACC GGTCTC(1/5)
1959 GAGACC
BsmBI 2342 GAGACG CGTCTC(1/5)
2548 GAGACG
BsmI 270 GAATGC GAATGC(1/-1)
1313 GAATGC
Bsp1407I 162 TGTACA T^GTACA
2314 TGTACA
BsrDI 2288 CATTGC GCAATG(2/0)
3300 CATTGC
BsrGI 162 TGTACA T^GTACA
2314 TGTACA
DraIII 2271 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
3511 CACTACGTG
EarI 458 CTCTTC CTCTTC(1/4)
1806 GAAGAG
EcoNI 203 CCTCTGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2510 CCTCGGCGAGG
FbaI 757 TGATCA T^GATCA
1559 TGATCA
HincII 2567 GTTGAC GT[CT]^[GA]AC
6112 GTCAAC
HindII 2567 GTTGAC GT[CT]^[GA]AC
6112 GTCAAC
Ksp632I 458 CTCTTC CTCTTC(1/4)
1806 GAAGAG
MamI 3990 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4174 GATCCCCATC
NcoI 954 CCATGG C^CATGG
1474 CCATGG
PaeR7I 1042 CTCGAG C^TCGAG
2141 CTCGAG
PshBI 1284 ATTAAT AT^TAAT
1596 ATTAAT
PvuII 417 CAGCTG CAG^CTG
427 CAGCTG
SexAI 1966 ACCAGGT A^CC[AT]GGT
3110 ACCAGGT
SspBI 162 TGTACA T^GTACA
2314 TGTACA
XhoI 1042 CTCGAG C^TCGAG
2141 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApoI AspHI AspI
AvaI AvaII BanII BbsI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrBI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflFI
PleI PpuMI PstI RcaI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XbaI XcmI XhoII