##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
Alw44I ApaLI AscI Asp718I BalI
BbeI BbrPI BcgI BciVI BsaHI
BsiWI BspDI BssHII Bst1107I BstEII
BstPI BstZ17I ClaI CpoI DraIII
DrdI EagI EclXI Eco52I EcoNI
EcoO65I FseI Hin1I HpaI KasI
KpnI MluI MluNI MscI NarI
NheI NotI NruI PacI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
RsrII SalI SbfI ScaI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I StuI SwaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccII 132 CGCG CG^CG
AhdI 253 GACAGACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2161 CCTGAGG CC^T[ACGT]AGG
Aor51HI 42 AGCGCT AGC^GCT
ApaI 2788 GGGCCC GGGCC^C
Asp700I 1140 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 253 GACAGACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1666 GAGCTC G[AT]GC[AT]^C
AspI 2858 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4578 TGCGCA TGC^GCA
BamHI 4665 GGATCC G^GATCC
BglI 2790 GCCCGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 3722 TACGTG [CT]AC^GT[GA]
BsaI 296 GGTCTC GGTCTC(1/5)
BsgI 5942 GTGCAG GTGCAG(16/14)
BsiHKAI 1666 GAGCTC G[AT]GC[AT]^C
BspHI 2416 TCATGA T^CATGA
BspLU11I 2590 ACATGT A^CATGT
BsrBI 5897 GAGCGG CCGCTC(-3/-3)
BsrFI 440 GCCGGC [GA]^CCGG[CT]
BssSI 1752 CTCGTG CACGAG(-5/-1)
BstAPI 5474 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4506 TTCGAA TT^CGAA
BstUI 132 CGCG CG^CG
Bsu36I 2161 CCTGAGG CC^T[ACGT]AGG
Cfr10I 440 GCCGGC [GA]^CCGG[CT]
EaeI 9 TGGCCG [CT]^GGCC[GA]
Eam1105I 253 GACAGACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 42 AGCGCT AGC^GCT
Eco81I 2161 CCTGAGG CC^T[ACGT]AGG
EcoRI 2648 GAATTC G^AATTC
EcoRV 1827 GATATC GAT^ATC
EcoT22I 2286 ATGCAT ATGCA^T
FauI 130 CCCGC CCCGC(4/6)
FspI 4578 TGCGCA TGC^GCA
HaeII 42 AGCGCT [GA]GCGC^[CT]
HincII 3207 GTTGAC GT[CT]^[GA]AC
HindII 3207 GTTGAC GT[CT]^[GA]AC
KspI 131 CCGCGG CCGC^GG
MfeI 5916 CAATTG C^AATTG
MunI 5916 CAATTG C^AATTG
MvnI 132 CGCG CG^CG
NaeI 440 GCCGGC GCC^GGC
NcoI 1016 CCATGG C^CATGG
NgoMIV 440 GCCGGC G^CCGGC
NsiI 2286 ATGCAT ATGCA^T
NspV 4506 TTCGAA TT^CGAA
PaeR7I 2428 CTCGAG C^TCGAG
PflFI 2858 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1641 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 4949 GGGACCT [GA]G^G[AT]CC[CT]
PvuI 175 CGATCG CGAT^CG
RcaI 2416 TCATGA T^CATGA
SacI 1666 GAGCTC GAGCT^C
SacII 131 CCGCGG CCGC^GG
SexAI 2573 ACCTGGT A^CC[AT]GGT
SfuI 4506 TTCGAA TT^CGAA
Tth111I 2858 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1641 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2428 CTCGAG C^TCGAG
XmnI 1140 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 1744 GTAGAC GT^[AC][GT]AC
5346 GTCTAC
AccIII 6050 TCCGGA T^CCGGA
6302 TCCGGA
AflII 2364 CTTAAG C^TTAAG
4853 CTTAAG
AflIII 2590 ACATGT A^C[GA][CT]GT
3723 ACGTGT
AseI 2912 ATTAAT AT^TAAT
3663 ATTAAT
AsnI 2912 ATTAAT AT^TAAT
3663 ATTAAT
AvrII 1832 CCTAGG C^CTAGG
1850 CCTAGG
BanI 118 GGCACC G^G[CT][GA]CC
5600 GGCACC
BanII 1666 GAGCTC G[GA]GC[CT]^C
2788 GGGCCC
BbsI 6354 GAAGAC GAAGAC(2/6)
4848 GTCTTC
BbvCI 2767 GCTGAGG CCTCAGC(-5/-2)
5231 GCTGAGG
BfrI 2364 CTTAAG C^TTAAG
4853 CTTAAG
BlnI 1832 CCTAGG C^CTAGG
1850 CCTAGG
BlpI 72 GCTCAGC GC^T[ACGT]AGC
2535 GCTGAGC
Bpu1102I 72 GCTCAGC GC^T[ACGT]AGC
2535 GCTGAGC
BpuAI 6354 GAAGAC GAAGAC(2/6)
4848 GTCTTC
BseAI 6050 TCCGGA T^CCGGA
6302 TCCGGA
BsiEI 134 CGGTCG CG[GA][CT]^CG
175 CGATCG
BsmI 3588 GAATGC GAATGC(1/-1)
2137 GCATTC
BspEI 6050 TCCGGA T^CCGGA
6302 TCCGGA
CelII 72 GCTCAGC GC^T[ACGT]AGC
2535 GCTGAGC
CfoI 43 GCGC GCG^C
4579 GCGC
DsaI 131 CCGCGG C^C[GA][CT]GG
1016 CCATGG
HgaI 416 GACGC GACGC(5/10)
342 GCGTC
HhaI 43 GCGC GCG^C
4579 GCGC
HinP1I 43 GCGC G^CGC
4579 GCGC
MroI 6050 TCCGGA T^CCGGA
6302 TCCGGA
NdeI 3568 CATATG CA^TATG
4518 CATATG
PshBI 2912 ATTAAT AT^TAAT
3663 ATTAAT
PvuII 18 CAGCTG CAG^CTG
2407 CAGCTG
SmaI 185 CCCGGG CCC^GGG
2791 CCCGGG
XcmI 1252 CCAATTAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2986 CCAGTTTTGGCCTGG
XmaCI 185 CCCGGG C^CCGGG
2791 CCCGGG
XmaI 185 CCCGGG C^CCGGG
2791 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaI AvaII BbvI
BclI BcnI BfaI BglII BmyI
BpmI BsaBI BsaJI BsaWI BseRI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII EarI EcoO109I
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII