##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
Acc65I AclI AgeI Alw44I ApaLI
AscI Asp718I AviII BsiEI BsiWI
BsmI BspDI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraIII EagI
EclXI Eco52I EcoRV EcoT22I FseI
FspI HpaI KpnI KspI MluI
NaeI NgoMIV NheI NotI NruI
NsiI NspV PaeR7I PinAI PmeI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SfiI SfuI SgrAI
SnaBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 1371 GACGTC GACGT^C
AccIII 3195 TCCGGA T^CCGGA
AflII 4829 CTTAAG C^TTAAG
AhdI 4589 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2162 AGCGCT AGC^GCT
Asp700I 2600 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4589 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2285 CCTAGG C^CTAGG
BamHI 5051 GGATCC G^GATCC
BbeI 1137 GGCGCC GGCGC^C
BbsI 1374 GTCTTC GAAGAC(2/6)
BfrI 4829 CTTAAG C^TTAAG
BlnI 2285 CCTAGG C^CTAGG
BlpI 667 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 667 GCTAAGC GC^T[ACGT]AGC
BpuAI 1374 GTCTTC GAAGAC(2/6)
BseAI 3195 TCCGGA T^CCGGA
Bsp1407I 6054 TGTACA T^GTACA
BspEI 3195 TCCGGA T^CCGGA
BsrBI 2986 CCGCTC CCGCTC(-3/-3)
BsrDI 5230 CATTGC GCAATG(2/0)
BsrFI 4566 GCCGGT [GA]^CCGG[CT]
BsrGI 6054 TGTACA T^GTACA
BstEII 3817 GGTAACC G^GT[ACGT]ACC
BstPI 3817 GGTAACC G^GT[ACGT]ACC
CelII 667 GCTAAGC GC^T[ACGT]AGC
Cfr10I 4566 GCCGGT [GA]^CCGG[CT]
Eam1105I 4589 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2162 AGCGCT AGC^GCT
EcoNI 3433 CCTTGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3817 GGTAACC G^GT[ACGT]ACC
EcoRI 2604 GAATTC G^AATTC
KasI 1137 GGCGCC G^GCGCC
MfeI 5530 CAATTG C^AATTG
MroI 3195 TCCGGA T^CCGGA
MunI 5530 CAATTG C^AATTG
NarI 1137 GGCGCC GG^CGCC
NdeI 6322 CATATG CA^TATG
SapI 722 GCTCTTC GCTCTTC(1/4)
SbfI 3823 CCTGCAGG CCTGCA^GG
ScaI 1961 AGTACT AGT^ACT
SexAI 5376 ACCTGGT A^CC[AT]GGT
SfoI 1137 GGCGCC GGC^GCC
SpeI 801 ACTAGT A^CTAGT
SphI 5735 GCATGC GCATG^C
Sse8387I 3823 CCTGCAGG CCTGCA^GG
SspBI 6054 TGTACA T^GTACA
SspI 420 AATATT AAT^ATT
TaqI 1582 TCGA T^CGA
TthHB8I 1582 TCGA T^CGA
XbaI 5064 TCTAGA T^CTAGA
XmnI 2600 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 1274 CGCG CG^CG
5820 CGCG
ApaI 297 GGGCCC GGGCC^C
715 GGGCCC
AseI 1506 ATTAAT AT^TAAT
1571 ATTAAT
AsnI 1506 ATTAAT AT^TAAT
1571 ATTAAT
BbvCI 4270 CCTCAGC CCTCAGC(-5/-2)
4405 CCTCAGC
BcgI 4259 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4107 GCAGTGGCCTCG
BciVI 2370 GGATAC GTATCC(6/5)
2936 GTATCC
BclI 116 TGATCA T^GATCA
2709 TGATCA
BssSI 2295 CACGAG CACGAG(-5/-1)
2893 CACGAG
BstUI 1274 CGCG CG^CG
5820 CGCG
DrdI 3522 GACACAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3561 GACTTTGCGGTC
FbaI 116 TGATCA T^GATCA
2709 TGATCA
HgaI 3795 GACGC GACGC(5/10)
1631 GCGTC
HincII 2399 GTCAAC GT[CT]^[GA]AC
5427 GTCAAC
HindII 2399 GTCAAC GT[CT]^[GA]AC
5427 GTCAAC
MvnI 1274 CGCG CG^CG
5820 CGCG
NcoI 1674 CCATGG C^CATGG
6403 CCATGG
PacI 1572 TTAATTAA TTAAT^TAA
6335 TTAATTAA
PflMI 2760 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4526 CCATAAACTGG
PshBI 1506 ATTAAT AT^TAAT
1571 ATTAAT
StuI 2762 AGGCCT AGG^CCT
6129 AGGCCT
Van91I 2760 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4526 CCATAAACTGG
XcmI 370 CCACCTGCAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5576 CCACAGGGAGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AspI AvaI AvaII
BalI BanI BanII BbrPI BbvI
BcnI BfaI BglI BglII BmyI
BpmI BsaAI BsaBI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspHI BspLU11I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflFI PleI
PmaCI PmlI PpuMI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I XhoII XmaCI XmaI