##################################
# NO CUT ENZYMES (   69 enzymes) #
##################################

AccII       AgeI        AhdI        ApaI        AscI        
AspEI       AviII       AvrII       BamHI       BbvCI       
BglI        BlnI        BsiEI       BsiWI       BsmBI       
BspDI       BspLU11I    BsrBI       BsrFI       BssHII      
BstBI       BstEII      BstPI       BstUI       Cfr10I      
ClaI        CpoI        DrdI        EagI        Eam1105I    
EclXI       Eco52I      EcoO65I     EcoRV       FseI        
FspI        HgaI        HpaI        KspI        MluI        
MvnI        NaeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PinAI       
PmeI        PshAI       PvuI        RsrII       SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SnaBI       Sse8387I    
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AatII         604  GACGTC               GACGT^C
Acc65I        529  GGTACC               G^GTACC
AccIII       3052  TCCGGA               T^CCGGA
AclI         5761  AACGTT               AA^CGTT
AflIII       1815  ACACGT               A^C[GA][CT]GT
Aor51HI      3835  AGCGCT               AGC^GCT
AseI         4209  ATTAAT               AT^TAAT
AsnI         4209  ATTAAT               AT^TAAT
Asp700I       988  GAAAGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       529  GGTACC               G^GTACC
AspI          417  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         3009  CTCGGG               C^[CT]CG[GA]G
AvaII        3688  GGACC                G^G[AT]CC
BcgI         4536  GCAAAGTATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         6000  TGATCA               T^GATCA
BsaWI        3052  TCCGGA               [AT]^CCGG[AT]
BseAI        3052  TCCGGA               T^CCGGA
BsoBI        3009  CTCGGG               C^[CT]CG[GA]G
BspEI        3052  TCCGGA               T^CCGGA
BssSI        3062  CTCGTG               CACGAG(-5/-1)
Bst1107I     6081  GTATAC               GTA^TAC
BstXI        2340  CCAGAGCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      6081  GTATAC               GTA^TAC
DraII        3687  GGGACCT              [GA]G^G[ACGT]CC[CT]
DraIII       1864  CACAAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          788  CCATGG               C^C[GA][CT]GG
Eco47III     3835  AGCGCT               AGC^GCT
EcoO109I     3687  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRI        2107  GAATTC               G^AATTC
FbaI         6000  TGATCA               T^GATCA
KpnI          529  GGTACC               GGTAC^C
MroI         3052  TCCGGA               T^CCGGA
NcoI          788  CCATGG               C^CATGG
NspI         2481  GCATGC               [GA]CATG^[CT]
PflFI         417  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        4723  CCATATGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        3687  GGGACCT              [GA]G^G[AT]CC[CT]
PshBI        4209  ATTAAT               AT^TAAT
Psp1406I     5761  AACGTT               AA^CGTT
SpeI         4447  ACTAGT               A^CTAGT
SphI         2481  GCATGC               GCATG^C
Tth111I       417  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       4723  CCATATGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         2754  TCTAGA               T^CTAGA
XmnI          988  GAAAGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccI          167  GTAGAC               GT^[AC][GT]AC
             6081  GTATAC              
AocI           44  CCTCAGG              CC^T[ACGT]AGG
              658  CCTCAGG             
BalI         2880  TGGCCA               TGG^CCA
             3150  TGGCCA              
BbeI         2329  GGCGCC               GGCGC^C
             4440  GGCGCC              
BciVI        3815  GTATCC               GTATCC(6/5)
             4673  GTATCC              
BlpI          322  GCTGAGC              GC^T[ACGT]AGC
             1624  GCTGAGC             
Bpu1102I      322  GCTGAGC              GC^T[ACGT]AGC
             1624  GCTGAGC             
BsaBI        2029  GATAAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4972  GATAGAAATC          
BspHI         968  TCATGA               T^CATGA
             3410  TCATGA              
Bsu36I         44  CCTCAGG              CC^T[ACGT]AGG
              658  CCTCAGG             
CelII         322  GCTGAGC              GC^T[ACGT]AGC
             1624  GCTGAGC             
Eco81I         44  CCTCAGG              CC^T[ACGT]AGG
              658  CCTCAGG             
KasI         2329  GGCGCC               G^GCGCC
             4440  GGCGCC              
MamI         2029  GATAAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4972  GATAGAAATC          
MfeI         1610  CAATTG               C^AATTG
             1994  CAATTG              
MluNI        2880  TGGCCA               TGG^CCA
             3150  TGGCCA              
MscI         2880  TGGCCA               TGG^CCA
             3150  TGGCCA              
MunI         1610  CAATTG               C^AATTG
             1994  CAATTG              
NarI         2329  GGCGCC               GG^CGCC
             4440  GGCGCC              
RcaI          968  TCATGA               T^CATGA
             3410  TCATGA              
ScaI          617  AGTACT               AGT^ACT
             5715  AGTACT              
SfoI         2329  GGCGCC               GGC^GCC
             4440  GGCGCC              
StuI         1762  AGGCCT               AGG^CCT
             2789  AGGCCT              
SwaI         1127  ATTTAAAT             ATTT^AAAT
             4230  ATTTAAAT            


#########################################
# 3 OR MORE CUT ENZYMES (  120 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AflII       
AluI        Alw44I      AlwI        AlwNI       ApaLI       
ApoI        AspHI       BanI        BanII       BbrPI       
BbsI        BbvI        BcnI        BfaI        BfrI        
BglII       BmyI        BpmI        BpuAI       BsaAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    Bsp1407I    BspMI       BsrDI       
BsrGI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        EaeI        EarI        
EcoNI       EcoRII      EcoT14I     EcoT22I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeI        NdeII       NlaIII      NlaIV       NsiI        
PleI        PmaCI       PmlI        PstI        PvuII       
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspBI       SspI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII