##################################
# NO CUT ENZYMES ( 39 enzymes) #
##################################
Acc65I AclI Alw44I AocI ApaLI
AscI Asp718I AvrII BbrPI BlnI
BsiWI BsmI BssHII Bst1107I BstZ17I
Bsu36I CpoI Eco81I FseI HpaI
KpnI MluI NheI NotI NruI
PacI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SalI SgrAI SnaBI
SpeI SphI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 4382 GACGTC GACGT^C
AccI 2968 GTCTAC GT^[AC][GT]AC
AflII 373 CTTAAG C^TTAAG
AgeI 3034 ACCGGT A^CCGGT
Aor51HI 2946 AGCGCT AGC^GCT
AviII 5375 TGCGCA TGC^GCA
BbeI 6080 GGCGCC GGCGC^C
BcgI 4865 CGACGGCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 373 CTTAAG C^TTAAG
BglI 4272 GCCTCACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3762 AGATCT A^GATCT
BsaAI 1454 TACGTG [CT]AC^GT[GA]
BsaBI 1760 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 5992 ATCGAT AT^CGAT
BspHI 6154 TCATGA T^CATGA
BsrDI 3494 GCAATG GCAATG(2/0)
BstAPI 5781 GCACTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1692 TTCGAA TT^CGAA
BstEII 3112 GGTCACC G^GT[ACGT]ACC
BstPI 3112 GGTCACC G^GT[ACGT]ACC
ClaI 5992 ATCGAT AT^CGAT
DraI 2918 TTTAAA TTT^AAA
Eco47III 2946 AGCGCT AGC^GCT
EcoO65I 3112 GGTCACC G^GT[ACGT]ACC
EcoRI 2068 GAATTC G^AATTC
FspI 5375 TGCGCA TGC^GCA
KasI 6080 GGCGCC G^GCGCC
KspI 272 CCGCGG CCGC^GG
MamI 1760 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 197 CAATTG C^AATTG
MunI 197 CAATTG C^AATTG
NarI 6080 GGCGCC GG^CGCC
NdeI 3869 CATATG CA^TATG
NspV 1692 TTCGAA TT^CGAA
PinAI 3034 ACCGGT A^CCGGT
PshAI 984 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 6154 TCATGA T^CATGA
SacII 272 CCGCGG CCGC^GG
SbfI 3478 CCTGCAGG CCTGCA^GG
SfiI 4271 GGCCTCACCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 6080 GGCGCC GGC^GCC
SfuI 1692 TTCGAA TT^CGAA
Sse8387I 3478 CCTGCAGG CCTGCA^GG
TfiI 3648 GAATC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 1572 TCCGGA T^CCGGA
4059 TCCGGA
AseI 6061 ATTAAT AT^TAAT
6594 ATTAAT
AsnI 6061 ATTAAT AT^TAAT
6594 ATTAAT
Asp700I 2064 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6233 GAATTGTTTC
AspI 2393 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2665 GACGCCGTC
BamHI 3573 GGATCC G^GATCC
4605 GGATCC
BbvCI 1096 CCTCAGC CCTCAGC(-5/-2)
875 GCTGAGG
BciVI 3267 GGATAC GTATCC(6/5)
5037 GGATAC
BseAI 1572 TCCGGA T^CCGGA
4059 TCCGGA
Bsp1407I 4596 TGTACA T^GTACA
6544 TGTACA
BspEI 1572 TCCGGA T^CCGGA
4059 TCCGGA
BsrBI 2743 CCGCTC CCGCTC(-3/-3)
3533 GAGCGG
BsrGI 4596 TGTACA T^GTACA
6544 TGTACA
DrdI 3152 GACATGCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3902 GACCATGTGGTC
EagI 1239 CGGCCG C^GGCCG
3513 CGGCCG
EclXI 1239 CGGCCG C^GGCCG
3513 CGGCCG
Eco52I 1239 CGGCCG C^GGCCG
3513 CGGCCG
EcoNI 5762 CCTTGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5887 CCTTTCCCAGG
EcoRV 1568 GATATC GAT^ATC
3071 GATATC
EcoT22I 3155 ATGCAT ATGCA^T
5988 ATGCAT
MroI 1572 TCCGGA T^CCGGA
4059 TCCGGA
NsiI 3155 ATGCAT ATGCA^T
5988 ATGCAT
PflFI 2393 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2665 GACGCCGTC
PshBI 6061 ATTAAT AT^TAAT
6594 ATTAAT
SapI 1894 GAAGAGC GCTCTTC(1/4)
774 GCTCTTC
ScaI 3801 AGTACT AGT^ACT
6102 AGTACT
SexAI 2651 ACCTGGT A^CC[AT]GGT
4626 ACCTGGT
SmaI 2264 CCCGGG CCC^GGG
2779 CCCGGG
SspBI 4596 TGTACA T^GTACA
6544 TGTACA
Tth111I 2393 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2665 GACGCCGTC
XmaCI 2264 CCCGGG C^CCGGG
2779 CCCGGG
XmaI 2264 CCCGGG C^CCGGG
2779 CCCGGG
XmnI 2064 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6233 GAATTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
ApaI ApoI AspEI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BclI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspLU11I
BspMI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI Eam1105I EarI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PaeR7I
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoI XhoII