##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspI BcgI BclI BfrI BsaBI
BsiEI BsiWI BspDI BspHI BspLU11I
BsrFI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I Cfr10I ClaI CpoI
DraI DraIII EagI EclXI Eco47III
Eco52I EcoRV EcoT22I FbaI FseI
HpaI KpnI KspI MamI MfeI
MluI MunI MvnI NaeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SfiI SfuI SgrAI
SnaBI SpeI SphI SspI SwaI
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 4232 TCCGGA T^CCGGA
AcyI 3369 GGCGCC G[GA]^CG[CT]C
AflIII 250 ACGTGT A^C[GA][CT]GT
AviII 3803 TGCGCA TGC^GCA
AvrII 4342 CCTAGG C^CTAGG
BbeI 3369 GGCGCC GGCGC^C
BbrPI 249 CACGTG CAC^GTG
BglII 4736 AGATCT A^GATCT
BlnI 4342 CCTAGG C^CTAGG
BsaAI 249 CACGTG [CT]AC^GT[GA]
BsaHI 3369 GGCGCC G[GA]^CG[CT]C
BseAI 4232 TCCGGA T^CCGGA
Bsp1407I 289 TGTACA T^GTACA
BspEI 4232 TCCGGA T^CCGGA
BsrBI 2208 CCGCTC CCGCTC(-3/-3)
BsrGI 289 TGTACA T^GTACA
DrdI 2948 GACAGCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3828 GAATTC G^AATTC
FspI 3803 TGCGCA TGC^GCA
HgaI 148 GCGTC GACGC(5/10)
Hin1I 3369 GGCGCC G[GA]^CG[CT]C
HincII 3690 GTTGAC GT[CT]^[GA]AC
HindII 3690 GTTGAC GT[CT]^[GA]AC
HindIII 4566 AAGCTT A^AGCTT
KasI 3369 GGCGCC G^GCGCC
MroI 4232 TCCGGA T^CCGGA
NarI 3369 GGCGCC GG^CGCC
NheI 3058 GCTAGC G^CTAGC
PmaCI 249 CACGTG CAC^GTG
PmlI 249 CACGTG CAC^GTG
ScaI 2373 AGTACT AGT^ACT
SexAI 535 ACCAGGT A^CC[AT]GGT
SfoI 3369 GGCGCC GGC^GCC
SmaI 676 CCCGGG CCC^GGG
SspBI 289 TGTACA T^GTACA
TaqI 299 TCGA T^CGA
TthHB8I 299 TCGA T^CGA
XmaCI 676 CCCGGG C^CCGGG
XmaI 676 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 1756 GTCTAC GT^[AC][GT]AC
2796 GTCTAC
AhdI 4 GACTCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
780 GACCTCTGGTC
AspEI 4 GACTCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
780 GACCTCTGGTC
BamHI 3591 GGATCC G^GATCC
3954 GGATCC
BciVI 3308 GTATCC GTATCC(6/5)
4082 GTATCC
BglI 3809 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4000 GCCTACCAGGC
BlpI 3237 GCTGAGC GC^T[ACGT]AGC
3921 GCTCAGC
Bpu1102I 3237 GCTGAGC GC^T[ACGT]AGC
3921 GCTCAGC
BsaI 1509 GAGACC GGTCTC(1/5)
4190 GAGACC
BsaWI 1378 ACCGGA [AT]^CCGG[AT]
4232 TCCGGA
BstEII 2151 GGTAACC G^GT[ACGT]ACC
2967 GGTGACC
BstPI 2151 GGTAACC G^GT[ACGT]ACC
2967 GGTGACC
CelII 3237 GCTGAGC GC^T[ACGT]AGC
3921 GCTCAGC
Eam1105I 4 GACTCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
780 GACCTCTGGTC
EcoO65I 2151 GGTAACC G^GT[ACGT]ACC
2967 GGTGACC
MslI 2363 CATGTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2902 CATTCGCATG
NdeI 429 CATATG CA^TATG
2517 CATATG
SapI 1322 GAAGAGC GCTCTTC(1/4)
185 GCTCTTC
SbfI 926 CCTGCAGG CCTGCA^GG
1895 CCTGCAGG
Sse8387I 926 CCTGCAGG CCTGCA^GG
1895 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII