##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AclI AcsI AflIII AgeI
Aor51HI ApoI AscI AseI AsnI
AviII BamHI BglII BsaBI BsiEI
BsiWI BspDI BspLU11I BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FseI FspI KspI
MamI MfeI MluI MunI NaeI
NgoMIV NotI NruI NsiI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 1220 TCCGGA T^CCGGA
AflII 2246 CTTAAG C^TTAAG
Alw44I 6285 GTGCAC G^TGCAC
ApaLI 6285 GTGCAC G^TGCAC
Asp700I 3912 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 5174 CACGTG CAC^GTG
BcgI 4577 CGAGGAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2246 CTTAAG C^TTAAG
BlpI 1129 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1129 GCTAAGC GC^T[ACGT]AGC
BseAI 1220 TCCGGA T^CCGGA
BsmBI 6179 GAGACG CGTCTC(1/5)
BsmI 5843 GAATGC GAATGC(1/-1)
BspEI 1220 TCCGGA T^CCGGA
BspHI 2873 TCATGA T^CATGA
BstXI 3195 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1129 GCTAAGC GC^T[ACGT]AGC
DraI 4042 TTTAAA TTT^AAA
HindIII 5897 AAGCTT A^AGCTT
MroI 1220 TCCGGA T^CCGGA
NdeI 3700 CATATG CA^TATG
NheI 4634 GCTAGC G^CTAGC
PmaCI 5174 CACGTG CAC^GTG
PmlI 5174 CACGTG CAC^GTG
RcaI 2873 TCATGA T^CATGA
SacI 1224 GAGCTC GAGCT^C
SmaI 5209 CCCGGG CCC^GGG
XmaCI 5209 CCCGGG C^CCGGG
XmaI 5209 CCCGGG C^CCGGG
XmnI 3912 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AspI 6015 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
6241 GACTCTGTC
BbeI 2783 GGCGCC GGCGC^C
6137 GGCGCC
BbsI 3577 GTCTTC GAAGAC(2/6)
4187 GTCTTC
BclI 3964 TGATCA T^GATCA
4999 TGATCA
BpuAI 3577 GTCTTC GAAGAC(2/6)
4187 GTCTTC
BsaAI 3889 CACGTA [CT]AC^GT[GA]
5174 CACGTG
BsaWI 1220 TCCGGA [AT]^CCGG[AT]
3789 TCCGGT
Bsp1407I 2541 TGTACA T^GTACA
4273 TGTACA
BsrBI 2597 CCGCTC CCGCTC(-3/-3)
484 GAGCGG
BsrFI 302 ACCGGC [GA]^CCGG[CT]
1730 GCCGGT
BsrGI 2541 TGTACA T^GTACA
4273 TGTACA
BssSI 3295 CTCGTG CACGAG(-5/-1)
5096 CTCGTG
Cfr10I 302 ACCGGC [GA]^CCGG[CT]
1730 GCCGGT
DraIII 718 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
986 CACCAGGTG
FbaI 3964 TGATCA T^GATCA
4999 TGATCA
HincII 5686 GTTAAC GT[CT]^[GA]AC
5815 GTTAAC
HindII 5686 GTTAAC GT[CT]^[GA]AC
5815 GTTAAC
HpaI 5686 GTTAAC GTT^AAC
5815 GTTAAC
KasI 2783 GGCGCC G^GCGCC
6137 GGCGCC
MslI 58 CATGGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1619 CACACACATG
NarI 2783 GGCGCC GG^CGCC
6137 GGCGCC
PaeR7I 167 CTCGAG C^TCGAG
5382 CTCGAG
PflFI 6015 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
6241 GACTCTGTC
SfoI 2783 GGCGCC GGC^GCC
6137 GGCGCC
SspBI 2541 TGTACA T^GTACA
4273 TGTACA
Tth111I 6015 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
6241 GACTCTGTC
XbaI 3908 TCTAGA T^CTAGA
5258 TCTAGA
XhoI 167 CTCGAG C^TCGAG
5382 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
Acc65I AccI AccII AciI AcyI
AfaI AhdI AluI AlwI AlwNI
AocI ApaI Asp718I AspEI AspHI
AvaI AvaII AvrII BalI BanI
BanII BbvCI BbvI BciVI BcnI
BfaI BglI BlnI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI Eam1105I EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII