##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccI AccIII AcsI AflII AocI
ApoI AscI AseI AsnI AviII
BciVI BclI BfrI BsaBI BseAI
BspDI BspEI BspHI Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DraI
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI MamI
MfeI MluI MroI MunI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PmeI PshAI PshBI PvuI
RcaI RsrII SalI ScaI SexAI
SfuI SgrAI SnaBI SphI SspI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 3186 GACGTC GACGT^C
Acc65I 844 GGTACC G^GTACC
AclI 2154 AACGTT AA^CGTT
AgeI 2254 ACCGGT A^CCGGT
AhdI 624 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1602 AGCGCT AGC^GCT
Asp700I 1964 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 844 GGTACC G^GTACC
AspEI 624 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 3668 GGATCC G^GATCC
BbeI 2010 GGCGCC GGCGC^C
BbsI 3441 GAAGAC GAAGAC(2/6)
BbvCI 147 CCTCAGC CCTCAGC(-5/-2)
BglII 96 AGATCT A^GATCT
BlpI 2105 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2105 GCTGAGC GC^T[ACGT]AGC
BpuAI 3441 GAAGAC GAAGAC(2/6)
BsiEI 2179 CGGCCG CG[GA][CT]^CG
BsiWI 2317 CGTACG C^GTACG
BsmI 1226 GAATGC GAATGC(1/-1)
BspLU11I 2248 ACATGT A^CATGT
BsrDI 572 GCAATG GCAATG(2/0)
BssHII 2220 GCGCGC G^CGCGC
CelII 2105 GCTGAGC GC^T[ACGT]AGC
DraIII 3605 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2179 CGGCCG C^GGCCG
Eam1105I 624 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2179 CGGCCG C^GGCCG
Eco47III 1602 AGCGCT AGC^GCT
Eco52I 2179 CGGCCG C^GGCCG
KasI 2010 GGCGCC G^GCGCC
KpnI 844 GGTACC GGTAC^C
KspI 2242 CCGCGG CCGC^GG
MseI 4040 TTAA T^TAA
NaeI 1785 GCCGGC GCC^GGC
NarI 2010 GGCGCC GG^CGCC
NgoMIV 1785 GCCGGC G^CCGGC
NheI 1316 GCTAGC G^CTAGC
NspI 2248 ACATGT [GA]CATG^[CT]
PinAI 2254 ACCGGT A^CCGGT
Psp1406I 2154 AACGTT AA^CGTT
SacII 2242 CCGCGG CCGC^GG
SapI 479 GCTCTTC GCTCTTC(1/4)
SbfI 3391 CCTGCAGG CCTGCA^GG
SfiI 1716 GGCCACCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2010 GGCGCC GGC^GCC
SmlI 447 CTTGAG C^T[CT][GA]AG
SpeI 431 ACTAGT A^CTAGT
Sse8387I 3391 CCTGCAGG CCTGCA^GG
Tru9I 4040 TTAA T^TAA
XmnI 1964 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Alw44I 2645 GTGCAC G^TGCAC
3149 GTGCAC
ApaLI 2645 GTGCAC G^TGCAC
3149 GTGCAC
AspI 972 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3210 GACATCGTC
AvrII 552 CCTAGG C^CTAGG
3409 CCTAGG
BlnI 552 CCTAGG C^CTAGG
3409 CCTAGG
BsaWI 1440 ACCGGA [AT]^CCGG[AT]
2254 ACCGGT
Bsp1407I 928 TGTACA T^GTACA
2539 TGTACA
BsrGI 928 TGTACA T^GTACA
2539 TGTACA
BstEII 1250 GGTGACC G^GT[ACGT]ACC
2675 GGTGACC
BstPI 1250 GGTGACC G^GT[ACGT]ACC
2675 GGTGACC
BstYI 96 AGATCT [GA]^GATC[CT]
3668 GGATCC
DrdI 426 GACCAACTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1833 GACCGCAACGTC
EcoO65I 1250 GGTGACC G^GT[ACGT]ACC
2675 GGTGACC
HincII 632 GTCAAC GT[CT]^[GA]AC
1884 GTTGAC
HindII 632 GTCAAC GT[CT]^[GA]AC
1884 GTTGAC
MflI 96 AGATCT [GA]^GATC[CT]
3668 GGATCC
PflFI 972 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3210 GACATCGTC
PpuMI 819 AGGACCT [GA]G^G[AT]CC[CT]
2091 GGGTCCT
SacI 2021 GAGCTC GAGCT^C
3929 GAGCTC
SspBI 928 TGTACA T^GTACA
2539 TGTACA
TfiI 58 GAATC G^A[AT]TC
1444 GAATC
Tsp509I 137 AATT ^AATT
199 AATT
Tth111I 972 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3210 GACATCGTC
XhoII 96 AGATCT [GA]^GATC[CT]
3668 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbrPI BbvI BcgI BcnI BfaI
BglI BmyI BpmI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PmaCI PmlI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StuI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I XcmI XmaCI XmaI