##################################
# NO CUT ENZYMES ( 43 enzymes) #
##################################
AatII AccIII AclI AflII Aor51HI
AscI AseI AsnI BciVI BclI
BfrI BseAI BsiWI BspDI BspEI
BssHII BstBI ClaI DraIII Eco47III
EcoRV FbaI MfeI MluI MroI
MunI NheI NotI NruI NspV
PacI PmeI PshBI Psp1406I PvuI
SalI SexAI SfuI SgrAI SnaBI
SpeI SspI SwaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AcsI 4211 GAATTC [GA]^AATT[CT]
AgeI 3761 ACCGGT A^CCGGT
AhdI 1868 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 853 GTGCAC G^TGCAC
ApaLI 853 GTGCAC G^TGCAC
ApoI 4211 GAATTC [GA]^AATT[CT]
Asp700I 3716 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1868 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1814 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 5379 CCTAGG C^CTAGG
BamHI 2152 GGATCC G^GATCC
BglII 201 AGATCT A^GATCT
BlnI 5379 CCTAGG C^CTAGG
BsaBI 1114 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrDI 1177 CATTGC GCAATG(2/0)
Bst1107I 3331 GTATAC GTA^TAC
BstZ17I 3331 GTATAC GTA^TAC
CpoI 2949 CGGACCG CG^G[AT]CCG
DrdI 5905 GACGGCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1868 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4211 GAATTC G^AATTC
FseI 2572 GGCCGGCC GGCCGG^CC
HindIII 1245 AAGCTT A^AGCTT
HpaI 5271 GTTAAC GTT^AAC
MamI 1114 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 4109 CATATG CA^TATG
PflFI 1814 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3761 ACCGGT A^CCGGT
RsrII 2949 CGGACCG CG^G[AT]CCG
SbfI 4049 CCTGCAGG CCTGCA^GG
ScaI 963 AGTACT AGT^ACT
SfiI 3238 GGCCTGCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1375 GCATGC GCATG^C
Sse8387I 4049 CCTGCAGG CCTGCA^GG
TfiI 3462 GATTC G^A[AT]TC
Tth111I 1814 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4392 TCTAGA T^CTAGA
XmnI 3716 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AviII 234 TGCGCA TGC^GCA
1173 TGCGCA
BbsI 146 GTCTTC GAAGAC(2/6)
6007 GTCTTC
BpuAI 146 GTCTTC GAAGAC(2/6)
6007 GTCTTC
BsaWI 2202 TCCGGT [AT]^CCGG[AT]
3761 ACCGGT
BsmI 1215 GAATGC GAATGC(1/-1)
2114 GAATGC
Bsp1407I 561 TGTACA T^GTACA
6265 TGTACA
BspLU11I 675 ACATGT A^CATGT
3488 ACATGT
BsrGI 561 TGTACA T^GTACA
6265 TGTACA
BssSI 4594 CACGAG CACGAG(-5/-1)
2938 CTCGTG
BstEII 907 GGTCACC G^GT[ACGT]ACC
2397 GGTCACC
BstPI 907 GGTCACC G^GT[ACGT]ACC
2397 GGTCACC
DraI 3631 TTTAAA TTT^AAA
4505 TTTAAA
EcoO65I 907 GGTCACC G^GT[ACGT]ACC
2397 GGTCACC
EcoT22I 3293 ATGCAT ATGCA^T
4454 ATGCAT
FspI 234 TGCGCA TGC^GCA
1173 TGCGCA
NsiI 3293 ATGCAT ATGCA^T
4454 ATGCAT
SspBI 561 TGTACA T^GTACA
6265 TGTACA
Tsp509I 3602 AATT ^AATT
4212 AATT
XcmI 858 CCAGCCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3844 CCAAGGCTGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 158 enzymes) #
#########################################
Acc65I AccI AccII AciI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApaI Asp718I AspHI AvaI
AvaII BalI BanI BanII BbeI
BbrPI BbvCI BbvI BcgI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
KpnI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PaeR7I PflMI
PleI PmaCI PmlI PpuMI PshAI
PstI PvuII RcaI RsaI SacI
SacII SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StuI StyI TaqI Tru9I TseI
Tsp45I TspRI TthHB8I Van91I XhoI
XhoII XmaCI XmaI