################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## Acc65I AclI Aor51HI AscI Asp718I AviII BalI BbeI BbrPI BcgI BglII BsiEI BsiWI BspDI BsrBI BsrDI BssHII BssSI BstBI BstEII BstPI ClaI CpoI DraIII EagI EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI HaeII HpaI KasI KpnI KspI MfeI MluI MluNI MscI MunI NaeI NarI NgoMIV NotI NspV PacI PaeR7I PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 3132 GACGTC GACGT^C AccIII 1139 TCCGGA T^CCGGA AflII 916 CTTAAG C^TTAAG AgeI 4923 ACCGGT A^CCGGT AhdI 3186 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2258 GTGCAC G^TGCAC ApaI 1849 GGGCCC GGGCC^C ApaLI 2258 GTGCAC G^TGCAC Asp700I 4939 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 3186 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 988 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 3945 CCTAGG C^CTAGG BamHI 2665 GGATCC G^GATCC BbvCI 1249 CCTCAGC CCTCAGC(-5/-2) BfrI 916 CTTAAG C^TTAAG BglI 1851 GCCCCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 3945 CCTAGG C^CTAGG BlpI 4091 GCTAAGC GC^T[ACGT]AGC Bpu1102I 4091 GCTAAGC GC^T[ACGT]AGC BsaAI 2380 TACGTG [CT]AC^GT[GA] BsaBI 2494 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 1139 TCCGGA T^CCGGA BspEI 1139 TCCGGA T^CCGGA BspHI 5688 TCATGA T^CATGA BsrFI 4923 ACCGGT [GA]^CCGG[CT] BstAPI 3036 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 4091 GCTAAGC GC^T[ACGT]AGC Cfr10I 4923 ACCGGT [GA]^CCGG[CT] DraI 3687 TTTAAA TTT^AAA DrdI 4998 GACTCATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 2587 CGGCCA [CT]^GGCC[GA] Eam1105I 3186 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 4234 GAATTC G^AATTC EcoT22I 430 ATGCAT ATGCA^T MamI 2494 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1139 TCCGGA T^CCGGA NdeI 5081 CATATG CA^TATG NruI 3205 TCGCGA TCG^CGA NsiI 430 ATGCAT ATGCA^T PflFI 988 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 4923 ACCGGT A^CCGGT PshAI 4728 GACGTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 5688 TCATGA T^CATGA ScaI 1043 AGTACT AGT^ACT SexAI 2323 ACCAGGT A^CC[AT]GGT SphI 2507 GCATGC GCATG^C Tth111I 988 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 49 CCATTTTTTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 4939 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 2994 CGCG CG^CG 3206 CGCG AocI 2711 CCTCAGG CC^T[ACGT]AGG 6163 CCTAAGG BciVI 2097 GTATCC GTATCC(6/5) 4751 GTATCC BclI 1504 TGATCA T^GATCA 2647 TGATCA BcnI 1373 CCCGG CC^[GC]GG 3277 CCCGG BsaWI 1139 TCCGGA [AT]^CCGG[AT] 4923 ACCGGT BseRI 74 CTCCTC GAGGAG(10/8) 2709 CTCCTC BsmBI 2080 CGTCTC CGTCTC(1/5) 4726 GAGACG Bsp1407I 4527 TGTACA T^GTACA 5544 TGTACA BspLU11I 3460 ACATGT A^CATGT 3735 ACATGT BsrGI 4527 TGTACA T^GTACA 5544 TGTACA Bst1107I 5523 GTATAC GTA^TAC 6144 GTATAC BstUI 2994 CGCG CG^CG 3206 CGCG BstZ17I 5523 GTATAC GTA^TAC 6144 GTATAC Bsu36I 2711 CCTCAGG CC^T[ACGT]AGG 6163 CCTAAGG Eco81I 2711 CCTCAGG CC^T[ACGT]AGG 6163 CCTAAGG FauI 1406 CCCGC CCCGC(4/6) 1469 CCCGC FbaI 1504 TGATCA T^GATCA 2647 TGATCA MslI 2171 CACAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 4171 CATCTCTGTG MvnI 2994 CGCG CG^CG 3206 CGCG NciI 1373 CCCGG CC^[GC]GG 3277 CCCGG NcoI 1083 CCATGG C^CATGG 2917 CCATGG NheI 2212 GCTAGC G^CTAGC 5483 GCTAGC PflMI 2185 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2934 CCAGGTTCTGG SspBI 4527 TGTACA T^GTACA 5544 TGTACA StuI 4649 AGGCCT AGG^CCT 5737 AGGCCT Van91I 2185 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2934 CCAGGTTCTGG XbaI 287 TCTAGA T^CTAGA 4992 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI AseI AsnI AspHI AvaI AvaII BanI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI PshBI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII