##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AclI Aor51HI AscI Asp718I
AviII BalI BbeI BbrPI BcgI
BglII BsiEI BsiWI BspDI BsrBI
BsrDI BssHII BssSI BstBI BstEII
BstPI ClaI CpoI DraIII EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
FseI FspI HaeII HpaI KasI
KpnI KspI MfeI MluI MluNI
MscI MunI NaeI NarI NgoMIV
NotI NspV PacI PaeR7I PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 3132 GACGTC GACGT^C
AccIII 1139 TCCGGA T^CCGGA
AflII 916 CTTAAG C^TTAAG
AgeI 4923 ACCGGT A^CCGGT
AhdI 3186 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2258 GTGCAC G^TGCAC
ApaI 1849 GGGCCC GGGCC^C
ApaLI 2258 GTGCAC G^TGCAC
Asp700I 4939 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3186 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 988 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3945 CCTAGG C^CTAGG
BamHI 2665 GGATCC G^GATCC
BbvCI 1249 CCTCAGC CCTCAGC(-5/-2)
BfrI 916 CTTAAG C^TTAAG
BglI 1851 GCCCCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3945 CCTAGG C^CTAGG
BlpI 4091 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 4091 GCTAAGC GC^T[ACGT]AGC
BsaAI 2380 TACGTG [CT]AC^GT[GA]
BsaBI 2494 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1139 TCCGGA T^CCGGA
BspEI 1139 TCCGGA T^CCGGA
BspHI 5688 TCATGA T^CATGA
BsrFI 4923 ACCGGT [GA]^CCGG[CT]
BstAPI 3036 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 4091 GCTAAGC GC^T[ACGT]AGC
Cfr10I 4923 ACCGGT [GA]^CCGG[CT]
DraI 3687 TTTAAA TTT^AAA
DrdI 4998 GACTCATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2587 CGGCCA [CT]^GGCC[GA]
Eam1105I 3186 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4234 GAATTC G^AATTC
EcoT22I 430 ATGCAT ATGCA^T
MamI 2494 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1139 TCCGGA T^CCGGA
NdeI 5081 CATATG CA^TATG
NruI 3205 TCGCGA TCG^CGA
NsiI 430 ATGCAT ATGCA^T
PflFI 988 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4923 ACCGGT A^CCGGT
PshAI 4728 GACGTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 5688 TCATGA T^CATGA
ScaI 1043 AGTACT AGT^ACT
SexAI 2323 ACCAGGT A^CC[AT]GGT
SphI 2507 GCATGC GCATG^C
Tth111I 988 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 49 CCATTTTTTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 4939 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 2994 CGCG CG^CG
3206 CGCG
AocI 2711 CCTCAGG CC^T[ACGT]AGG
6163 CCTAAGG
BciVI 2097 GTATCC GTATCC(6/5)
4751 GTATCC
BclI 1504 TGATCA T^GATCA
2647 TGATCA
BcnI 1373 CCCGG CC^[GC]GG
3277 CCCGG
BsaWI 1139 TCCGGA [AT]^CCGG[AT]
4923 ACCGGT
BseRI 74 CTCCTC GAGGAG(10/8)
2709 CTCCTC
BsmBI 2080 CGTCTC CGTCTC(1/5)
4726 GAGACG
Bsp1407I 4527 TGTACA T^GTACA
5544 TGTACA
BspLU11I 3460 ACATGT A^CATGT
3735 ACATGT
BsrGI 4527 TGTACA T^GTACA
5544 TGTACA
Bst1107I 5523 GTATAC GTA^TAC
6144 GTATAC
BstUI 2994 CGCG CG^CG
3206 CGCG
BstZ17I 5523 GTATAC GTA^TAC
6144 GTATAC
Bsu36I 2711 CCTCAGG CC^T[ACGT]AGG
6163 CCTAAGG
Eco81I 2711 CCTCAGG CC^T[ACGT]AGG
6163 CCTAAGG
FauI 1406 CCCGC CCCGC(4/6)
1469 CCCGC
FbaI 1504 TGATCA T^GATCA
2647 TGATCA
MslI 2171 CACAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4171 CATCTCTGTG
MvnI 2994 CGCG CG^CG
3206 CGCG
NciI 1373 CCCGG CC^[GC]GG
3277 CCCGG
NcoI 1083 CCATGG C^CATGG
2917 CCATGG
NheI 2212 GCTAGC G^CTAGC
5483 GCTAGC
PflMI 2185 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2934 CCAGGTTCTGG
SspBI 4527 TGTACA T^GTACA
5544 TGTACA
StuI 4649 AGGCCT AGG^CCT
5737 AGGCCT
Van91I 2185 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2934 CCAGGTTCTGG
XbaI 287 TCTAGA T^CTAGA
4992 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AseI AsnI AspHI AvaI AvaII
BanI BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PpuMI PshBI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII