##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BbeI BbrPI BcgI
BciVI BclI BfrI BglI BlnI
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmI BspEI BspHI
BsrDI BssHII Bst1107I BstBI BstZ17I
CpoI DrdI EagI Eam1105I EclXI
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NarI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccI 1062 GTCGAC GT^[AC][GT]AC
AccII 102 CGCG CG^CG
AflIII 427 ACATGT A^C[GA][CT]GT
AspI 2211 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 862 GGATCC G^GATCC
BbvCI 209 CCTCAGC CCTCAGC(-5/-2)
Bsp1407I 160 TGTACA T^GTACA
BspDI 1818 ATCGAT AT^CGAT
BspLU11I 427 ACATGT A^CATGT
BsrFI 1231 GCCGGC [GA]^CCGG[CT]
BsrGI 160 TGTACA T^GTACA
BssSI 446 CACGAG CACGAG(-5/-1)
BstAPI 1796 GCAGACTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1667 GGTCACC G^GT[ACGT]ACC
BstPI 1667 GGTCACC G^GT[ACGT]ACC
BstUI 102 CGCG CG^CG
Cfr10I 1231 GCCGGC [GA]^CCGG[CT]
ClaI 1818 ATCGAT AT^CGAT
DraI 57 TTTAAA TTT^AAA
EcoO65I 1667 GGTCACC G^GT[ACGT]ACC
MvnI 102 CGCG CG^CG
NaeI 1231 GCCGGC GCC^GGC
NcoI 1651 CCATGG C^CATGG
NdeI 1480 CATATG CA^TATG
NgoMIV 1231 GCCGGC G^CCGGC
PflFI 2211 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1661 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2641 CTGCAG CTGCA^G
SacI 941 GAGCTC GAGCT^C
SalI 1062 GTCGAC G^TCGAC
SfcI 2641 CTGCAG C^T[GA][CT]AG
SspBI 160 TGTACA T^GTACA
StuI 2224 AGGCCT AGG^CCT
Tth111I 2211 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 344 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AlwNI 696 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
2034 CAGGGTCTG
Aor51HI 631 AGCGCT AGC^GCT
868 AGCGCT
BglII 641 AGATCT A^GATCT
1106 AGATCT
BsmBI 3302 CGTCTC CGTCTC(1/5)
1405 GAGACG
DraIII 1035 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2716 CACCCTGTG
Eco47III 631 AGCGCT AGC^GCT
868 AGCGCT
HincII 1062 GTCGAC GT[CT]^[GA]AC
1176 GTCAAC
HindII 1062 GTCGAC GT[CT]^[GA]AC
1176 GTCAAC
PflMI 748 CCACTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
764 CCAGTATGTGG
PvuII 1854 CAGCTG CAG^CTG
2367 CAGCTG
SmlI 231 CTCAAG C^T[CT][GA]AG
1581 CTCAAG
Van91I 748 CCACTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
764 CCAGTATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcyI AfaI AluI Alw44I
AlwI AocI ApaI ApaLI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SmaI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmaCI XmaI