##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
Acc65I AccI AccIII AclI AgeI
AhdI AscI Asp718I AspEI AviII
BamHI BsaBI BseAI BsiEI BsiWI
BspDI BspEI BspLU11I Bst1107I BstEII
BstPI BstZ17I ClaI CpoI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRV
FseI FspI KpnI MamI MluI
MroI NdeI NheI NotI NruI
PacI PinAI PmeI PshAI Psp1406I
PvuI RsrII SalI SbfI SfiI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 5879 GACGTC GACGT^C
AflII 886 CTTAAG C^TTAAG
AflIII 4294 ACGTGT A^C[GA][CT]GT
Aor51HI 3585 AGCGCT AGC^GCT
Asp700I 2853 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 5672 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BfrI 886 CTTAAG C^TTAAG
BglII 3813 AGATCT A^GATCT
BsaWI 1610 TCCGGT [AT]^CCGG[AT]
BsmI 5545 GAATGC GAATGC(1/-1)
Bsp1407I 935 TGTACA T^GTACA
BspHI 5883 TCATGA T^CATGA
BsrFI 5271 GCCGGC [GA]^CCGG[CT]
BsrGI 935 TGTACA T^GTACA
BssHII 2910 GCGCGC G^CGCGC
BssSI 1382 CTCGTG CACGAG(-5/-1)
BstAPI 4419 GCACTGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1478 TTCGAA TT^CGAA
Cfr10I 5271 GCCGGC [GA]^CCGG[CT]
DrdI 6051 GACTGTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3585 AGCGCT AGC^GCT
KspI 4676 CCGCGG CCGC^GG
NaeI 5271 GCCGGC GCC^GGC
NgoMIV 5271 GCCGGC G^CCGGC
NspV 1478 TTCGAA TT^CGAA
PaeR7I 1067 CTCGAG C^TCGAG
PflFI 5672 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3421 CCACGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 5883 TCATGA T^CATGA
SacII 4676 CCGCGG CCGC^GG
SexAI 3514 ACCAGGT A^CC[AT]GGT
SfuI 1478 TTCGAA TT^CGAA
SphI 1847 GCATGC GCATG^C
SspBI 935 TGTACA T^GTACA
Tth111I 5672 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3421 CCACGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1067 CTCGAG C^TCGAG
XmnI 2853 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AocI 6115 CCTGAGG CC^T[ACGT]AGG
6288 CCTGAGG
AseI 4941 ATTAAT AT^TAAT
5625 ATTAAT
AsnI 4941 ATTAAT AT^TAAT
5625 ATTAAT
BbeI 415 GGCGCC GGCGC^C
3311 GGCGCC
BbsI 3595 GAAGAC GAAGAC(2/6)
5387 GTCTTC
BcgI 4703 CGAGGGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5753 CGATTCTCCTGC
BciVI 1667 GGATAC GTATCC(6/5)
3900 GTATCC
BclI 694 TGATCA T^GATCA
2313 TGATCA
BpuAI 3595 GAAGAC GAAGAC(2/6)
5387 GTCTTC
Bsu36I 6115 CCTGAGG CC^T[ACGT]AGG
6288 CCTGAGG
Eco81I 6115 CCTGAGG CC^T[ACGT]AGG
6288 CCTGAGG
EcoRI 3626 GAATTC G^AATTC
5744 GAATTC
EcoT22I 4197 ATGCAT ATGCA^T
5415 ATGCAT
FbaI 694 TGATCA T^GATCA
2313 TGATCA
HindIII 2854 AAGCTT A^AGCTT
3049 AAGCTT
HpaI 3641 GTTAAC GTT^AAC
5487 GTTAAC
KasI 415 GGCGCC G^GCGCC
3311 GGCGCC
MfeI 5474 CAATTG C^AATTG
5492 CAATTG
MunI 5474 CAATTG C^AATTG
5492 CAATTG
NarI 415 GGCGCC GG^CGCC
3311 GGCGCC
NcoI 3336 CCATGG C^CATGG
3930 CCATGG
NsiI 4197 ATGCAT ATGCA^T
5415 ATGCAT
PshBI 4941 ATTAAT AT^TAAT
5625 ATTAAT
ScaI 533 AGTACT AGT^ACT
5325 AGTACT
SfoI 415 GGCGCC GGC^GCC
3311 GGCGCC
XbaI 960 TCTAGA T^CTAGA
5391 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaI
ApaLI ApoI AspHI AvaI AvaII
AvrII BalI BanI BanII BbrPI
BbvCI BbvI BcnI BfaI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI