################################## # NO CUT ENZYMES ( 56 enzymes) # ################################## Acc65I AccI AccIII AclI AgeI AhdI AscI Asp718I AspEI AviII BamHI BsaBI BseAI BsiEI BsiWI BspDI BspEI BspLU11I Bst1107I BstEII BstPI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco52I EcoO65I EcoRV FseI FspI KpnI MamI MluI MroI NdeI NheI NotI NruI PacI PinAI PmeI PshAI Psp1406I PvuI RsrII SalI SbfI SfiI SgrAI SnaBI SpeI Sse8387I SspI SwaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 5879 GACGTC GACGT^C AflII 886 CTTAAG C^TTAAG AflIII 4294 ACGTGT A^C[GA][CT]GT Aor51HI 3585 AGCGCT AGC^GCT Asp700I 2853 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 5672 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BfrI 886 CTTAAG C^TTAAG BglII 3813 AGATCT A^GATCT BsaWI 1610 TCCGGT [AT]^CCGG[AT] BsmI 5545 GAATGC GAATGC(1/-1) Bsp1407I 935 TGTACA T^GTACA BspHI 5883 TCATGA T^CATGA BsrFI 5271 GCCGGC [GA]^CCGG[CT] BsrGI 935 TGTACA T^GTACA BssHII 2910 GCGCGC G^CGCGC BssSI 1382 CTCGTG CACGAG(-5/-1) BstAPI 4419 GCACTGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1478 TTCGAA TT^CGAA Cfr10I 5271 GCCGGC [GA]^CCGG[CT] DrdI 6051 GACTGTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3585 AGCGCT AGC^GCT KspI 4676 CCGCGG CCGC^GG NaeI 5271 GCCGGC GCC^GGC NgoMIV 5271 GCCGGC G^CCGGC NspV 1478 TTCGAA TT^CGAA PaeR7I 1067 CTCGAG C^TCGAG PflFI 5672 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 3421 CCACGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 5883 TCATGA T^CATGA SacII 4676 CCGCGG CCGC^GG SexAI 3514 ACCAGGT A^CC[AT]GGT SfuI 1478 TTCGAA TT^CGAA SphI 1847 GCATGC GCATG^C SspBI 935 TGTACA T^GTACA Tth111I 5672 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 3421 CCACGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1067 CTCGAG C^TCGAG XmnI 2853 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AocI 6115 CCTGAGG CC^T[ACGT]AGG 6288 CCTGAGG AseI 4941 ATTAAT AT^TAAT 5625 ATTAAT AsnI 4941 ATTAAT AT^TAAT 5625 ATTAAT BbeI 415 GGCGCC GGCGC^C 3311 GGCGCC BbsI 3595 GAAGAC GAAGAC(2/6) 5387 GTCTTC BcgI 4703 CGAGGGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 5753 CGATTCTCCTGC BciVI 1667 GGATAC GTATCC(6/5) 3900 GTATCC BclI 694 TGATCA T^GATCA 2313 TGATCA BpuAI 3595 GAAGAC GAAGAC(2/6) 5387 GTCTTC Bsu36I 6115 CCTGAGG CC^T[ACGT]AGG 6288 CCTGAGG Eco81I 6115 CCTGAGG CC^T[ACGT]AGG 6288 CCTGAGG EcoRI 3626 GAATTC G^AATTC 5744 GAATTC EcoT22I 4197 ATGCAT ATGCA^T 5415 ATGCAT FbaI 694 TGATCA T^GATCA 2313 TGATCA HindIII 2854 AAGCTT A^AGCTT 3049 AAGCTT HpaI 3641 GTTAAC GTT^AAC 5487 GTTAAC KasI 415 GGCGCC G^GCGCC 3311 GGCGCC MfeI 5474 CAATTG C^AATTG 5492 CAATTG MunI 5474 CAATTG C^AATTG 5492 CAATTG NarI 415 GGCGCC GG^CGCC 3311 GGCGCC NcoI 3336 CCATGG C^CATGG 3930 CCATGG NsiI 4197 ATGCAT ATGCA^T 5415 ATGCAT PshBI 4941 ATTAAT AT^TAAT 5625 ATTAAT ScaI 533 AGTACT AGT^ACT 5325 AGTACT SfoI 415 GGCGCC GGC^GCC 3311 GGCGCC XbaI 960 TCTAGA T^CTAGA 5391 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 138 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI Alw44I AlwI AlwNI ApaI ApaLI ApoI AspHI AvaI AvaII AvrII BalI BanI BanII BbrPI BbvCI BbvI BcnI BfaI BglI BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI