##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI Alw44I ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AvrII
BciVI BfrI BlnI BsaBI BsiWI
Bsp1407I BspDI BspHI BspLU11I BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DrdI EcoO65I
EcoRI EcoRV EcoT22I HincII HindII
HindIII HpaI KpnI MamI MfeI
MluI MseI MunI NheI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI ScaI SfuI SgrAI
SnaBI SpeI SspBI SspI SwaI
Tru9I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AccI 979 GTCTAC GT^[AC][GT]AC
AhdI 3772 GACGAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3772 GACGAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1223 TGCGCA TGC^GCA
BalI 527 TGGCCA TGG^CCA
BbsI 1009 GTCTTC GAAGAC(2/6)
BbvCI 2024 CCTCAGC CCTCAGC(-5/-2)
BclI 2180 TGATCA T^GATCA
BfaI 2413 CTAG C^TAG
BglII 4088 AGATCT A^GATCT
BpuAI 1009 GTCTTC GAAGAC(2/6)
BsmI 2245 GCATTC GAATGC(1/-1)
BsrDI 3909 CATTGC GCAATG(2/0)
BssHII 4082 GCGCGC G^CGCGC
BssSI 1054 CTCGTG CACGAG(-5/-1)
BstXI 695 CCACAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1423 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3772 GACGAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 2180 TGATCA T^GATCA
FseI 4305 GGCCGGCC GGCCGG^CC
FspI 1223 TGCGCA TGC^GCA
MaeI 2413 CTAG C^TAG
MluNI 527 TGGCCA TGG^CCA
MscI 527 TGGCCA TGG^CCA
NdeI 4114 CATATG CA^TATG
NotI 1 GCGGCCGC GC^GGCCGC
SphI 950 GCATGC GCATG^C
TfiI 1569 GATTC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 463 ACGTGT A^C[GA][CT]GT
3323 ACGTGT
AspI 679 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1003 GACCAGGTC
BsaI 2960 GAGACC GGTCTC(1/5)
3789 GAGACC
BsmBI 3666 CGTCTC CGTCTC(1/5)
2791 GAGACG
EcoT14I 596 CCATGG C^C[AT][AT]GG
1067 CCATGG
KspI 3689 CCGCGG CCGC^GG
4369 CCGCGG
NaeI 3643 GCCGGC GCC^GGC
4306 GCCGGC
NcoI 596 CCATGG C^CATGG
1067 CCATGG
NgoMIV 3643 GCCGGC G^CCGGC
4306 GCCGGC
PaeR7I 1097 CTCGAG C^TCGAG
4189 CTCGAG
PflFI 679 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1003 GACCAGGTC
SacII 3689 CCGCGG CCGC^GG
4369 CCGCGG
SapI 1923 GCTCTTC GCTCTTC(1/4)
2481 GCTCTTC
SfiI 3693 GGCCGAGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4258 GGCCAGGCAGGCC
SmlI 1097 CTCGAG C^T[CT][GA]AG
4189 CTCGAG
StyI 596 CCATGG C^C[AT][AT]GG
1067 CCATGG
Tsp509I 1411 AATT ^AATT
3391 AATT
Tth111I 679 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1003 GACCAGGTC
XcmI 221 CCATGCCCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1163 CCACCGCCTTCCTGG
XhoI 1097 CTCGAG C^TCGAG
4189 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI AlwI AlwNI AocI Aor51HI
ApaI AspHI AvaI AvaII BamHI
BanI BanII BbeI BbrPI BbvI
BcgI BcnI BglI BlpI BmyI
BpmI Bpu1102I BsaAI BsaHI BsaJI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspEI BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MroI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I SbfI
ScrFI SexAI SfaNI SfcI SfoI
SmaI Sse8387I StuI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XhoII
XmaCI XmaI