##################################
# NO CUT ENZYMES ( 28 enzymes) #
##################################
AhdI AspEI BciVI BlpI Bpu1102I
BsaBI BspDI Bst1107I BstZ17I CelII
ClaI CpoI Eam1105I EcoRI EcoRV
MamI NdeI NotI NruI PacI
PmeI PvuI RsrII SfiI SgrAI
SnaBI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 3550 GGTACC G^GTACC
AccIII 231 TCCGGA T^CCGGA
AclI 2811 AACGTT AA^CGTT
AflII 4724 CTTAAG C^TTAAG
AgeI 1831 ACCGGT A^CCGGT
Alw44I 6180 GTGCAC G^TGCAC
Aor51HI 344 AGCGCT AGC^GCT
ApaLI 6180 GTGCAC G^TGCAC
AscI 873 GGCGCGCC GG^CGCGCC
AseI 3276 ATTAAT AT^TAAT
AsnI 3276 ATTAAT AT^TAAT
Asp700I 3626 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3550 GGTACC G^GTACC
AvrII 2759 CCTAGG C^CTAGG
BamHI 4115 GGATCC G^GATCC
BbrPI 485 CACGTG CAC^GTG
BfrI 4724 CTTAAG C^TTAAG
BglII 2377 AGATCT A^GATCT
BlnI 2759 CCTAGG C^CTAGG
BseAI 231 TCCGGA T^CCGGA
BsiWI 1455 CGTACG C^GTACG
BspEI 231 TCCGGA T^CCGGA
BspLU11I 5751 ACATGT A^CATGT
BsrBI 970 GAGCGG CCGCTC(-3/-3)
BsrDI 4780 CATTGC GCAATG(2/0)
BssHII 874 GCGCGC G^CGCGC
BstBI 1163 TTCGAA TT^CGAA
EagI 3809 CGGCCG C^GGCCG
EclXI 3809 CGGCCG C^GGCCG
Eco47III 344 AGCGCT AGC^GCT
Eco52I 3809 CGGCCG C^GGCCG
EcoT22I 3325 ATGCAT ATGCA^T
FseI 5296 GGCCGGCC GGCCGG^CC
HindIII 845 AAGCTT A^AGCTT
HpaI 4234 GTTAAC GTT^AAC
KpnI 3550 GGTACC GGTAC^C
KspI 1945 CCGCGG CCGC^GG
MfeI 3268 CAATTG C^AATTG
MluI 801 ACGCGT A^CGCGT
MroI 231 TCCGGA T^CCGGA
MunI 3268 CAATTG C^AATTG
NheI 5370 GCTAGC G^CTAGC
NsiI 3325 ATGCAT ATGCA^T
NspV 1163 TTCGAA TT^CGAA
PaeR7I 3909 CTCGAG C^TCGAG
PinAI 1831 ACCGGT A^CCGGT
PmaCI 485 CACGTG CAC^GTG
PmlI 485 CACGTG CAC^GTG
PshBI 3276 ATTAAT AT^TAAT
Psp1406I 2811 AACGTT AA^CGTT
SacII 1945 CCGCGG CCGC^GG
SalI 1607 GTCGAC G^TCGAC
SfuI 1163 TTCGAA TT^CGAA
SphI 6736 GCATGC GCATG^C
XbaI 3356 TCTAGA T^CTAGA
XhoI 3909 CTCGAG C^TCGAG
XmnI 3626 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 2367 GACGTC GACGT^C
3054 GACGTC
BbeI 359 GGCGCC GGCGC^C
6786 GGCGCC
BbsI 2598 GAAGAC GAAGAC(2/6)
1741 GTCTTC
BcgI 777 CGAGCTCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
6526 GCACTGTAATCG
BclI 894 TGATCA T^GATCA
3349 TGATCA
BpuAI 2598 GAAGAC GAAGAC(2/6)
1741 GTCTTC
BsmBI 1429 CGTCTC CGTCTC(1/5)
2838 CGTCTC
Bsp1407I 1563 TGTACA T^GTACA
6682 TGTACA
BspHI 5344 TCATGA T^CATGA
6629 TCATGA
BsrGI 1563 TGTACA T^GTACA
6682 TGTACA
BstEII 1405 GGTCACC G^GT[ACGT]ACC
4577 GGTGACC
BstPI 1405 GGTCACC G^GT[ACGT]ACC
4577 GGTGACC
BstXI 879 CCAACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1680 CCACATTCCTGG
DrdI 1310 GACCTGAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3048 GACGCAGACGTC
EcoNI 6138 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
6296 CCTCCAGGAGG
EcoO65I 1405 GGTCACC G^GT[ACGT]ACC
4577 GGTGACC
FbaI 894 TGATCA T^GATCA
3349 TGATCA
KasI 359 GGCGCC G^GCGCC
6786 GGCGCC
NarI 359 GGCGCC GG^CGCC
6786 GGCGCC
RcaI 5344 TCATGA T^CATGA
6629 TCATGA
SapI 5365 GAAGAGC GCTCTTC(1/4)
250 GCTCTTC
SbfI 1822 CCTGCAGG CCTGCA^GG
2355 CCTGCAGG
SfoI 359 GGCGCC GGC^GCC
6786 GGCGCC
SmaI 1508 CCCGGG CCC^GGG
5527 CCCGGG
Sse8387I 1822 CCTGCAGG CCTGCA^GG
2355 CCTGCAGG
SspBI 1563 TGTACA T^GTACA
6682 TGTACA
XmaCI 1508 CCCGGG C^CCGGG
5527 CCCGGG
XmaI 1508 CCCGGG C^CCGGG
5527 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApaI ApoI AspHI AspI
AvaI AvaII AviII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PshAI PstI PvuII
RsaI SacI Sau3AI Sau96I ScaI
ScrFI SexAI SfaNI SfcI SmlI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII