##################################
# NO CUT ENZYMES (   71 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AgeI        
AscI        AseI        AsnI        AspI        BamHI       
BbeI        BbrPI       BcgI        BciVI       BcnI        
BfrI        BglII       BsaBI       BsaI        BseAI       
BsiWI       BspDI       BspEI       BsrFI       BssHII      
Cfr10I      ClaI        CpoI        DraIII      EagI        
EclXI       Eco52I      EcoRI       FseI        HpaI        
KasI        KspI        MamI        MfeI        MluI        
MroI        MunI        NaeI        NarI        NciI        
NdeI        NgoMIV      NheI        NotI        NruI        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    RsrII       
SacII       SalI        SfiI        SfoI        SgrAI       
SmaI        SnaBI       Tth111I     XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

Acc65I       4460  GGTACC               G^GTACC
AccI          441  GTATAC               GT^[AC][GT]AC
AccII         583  CGCG                 CG^CG
AcyI          752  GACGCC               G[GA]^CG[CT]C
AhdI         2799  GACACTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2399  GTGCAC               G^TGCAC
Aor51HI       611  AGCGCT               AGC^GCT
ApaLI        2399  GTGCAC               G^TGCAC
Asp700I      4513  GAATCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      4460  GGTACC               G^GTACC
AspEI        2799  GACACTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         713  TGCGCA               TGC^GCA
AvrII        2729  CCTAGG               C^CTAGG
BbsI         3082  GTCTTC               GAAGAC(2/6)
BlnI         2729  CCTAGG               C^CTAGG
BlpI          653  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      653  GCTCAGC              GC^T[ACGT]AGC
BpuAI        3082  GTCTTC               GAAGAC(2/6)
BsaAI        4322  TACGTG               [CT]AC^GT[GA]
BsaHI         752  GACGCC               G[GA]^CG[CT]C
BsiEI         126  CGATCG               CG[GA][CT]^CG
BsmI         2818  GCATTC               GAATGC(1/-1)
Bsp1407I     4357  TGTACA               T^GTACA
BsrDI         470  GCAATG               GCAATG(2/0)
BsrGI        4357  TGTACA               T^GTACA
BssSI        1058  CTCGTG               CACGAG(-5/-1)
Bst1107I      441  GTATAC               GTA^TAC
BstBI        1416  TTCGAA               TT^CGAA
BstEII        138  GGTCACC              G^GT[ACGT]ACC
BstPI         138  GGTCACC              G^GT[ACGT]ACC
BstUI         583  CGCG                 CG^CG
BstXI        4569  CCATGTCAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       441  GTATAC               GTA^TAC
CelII         653  GCTCAGC              GC^T[ACGT]AGC
DrdI         2682  GACACACCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     2799  GACACTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          705  CTCTTC               CTCTTC(1/4)
Eco47III      611  AGCGCT               AGC^GCT
EcoO65I       138  GGTCACC              G^GT[ACGT]ACC
EcoRV        1360  GATATC               GAT^ATC
EcoT22I      3635  ATGCAT               ATGCA^T
FspI          713  TGCGCA               TGC^GCA
Hin1I         752  GACGCC               G[GA]^CG[CT]C
HindIII      4336  AAGCTT               A^AGCTT
KpnI         4460  GGTACC               GGTAC^C
Ksp632I       705  CTCTTC               CTCTTC(1/4)
MvnI          583  CGCG                 CG^CG
NsiI         3635  ATGCAT               ATGCA^T
NspV         1416  TTCGAA               TT^CGAA
PpuMI        3939  AGGTCCT              [GA]G^G[AT]CC[CT]
PvuI          126  CGATCG               CGAT^CG
SapI          704  GCTCTTC              GCTCTTC(1/4)
SbfI         1549  CCTGCAGG             CCTGCA^GG
ScaI         2741  AGTACT               AGT^ACT
SexAI        2433  ACCAGGT              A^CC[AT]GGT
SfuI         1416  TTCGAA               TT^CGAA
Sse8387I     1549  CCTGCAGG             CCTGCA^GG
SspBI        4357  TGTACA               T^GTACA
SwaI         2567  ATTTAAAT             ATTT^AAAT
XbaI         3789  TCTAGA               T^CTAGA
XmnI         4513  GAATCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AocI         1132  CCTGAGG              CC^T[ACGT]AGG
             1204  CCTCAGG             
ApaI         1558  GGGCCC               GGGCC^C
             3482  GGGCCC              
AvaI          872  CTCGGG               C^[CT]CG[GA]G
             3151  CCCGAG              
BalI          249  TGGCCA               TGG^CCA
             1908  TGGCCA              
BbvCI        1296  CCTCAGC              CCTCAGC(-5/-2)
             2246  GCTGAGG             
BglI          671  GCCAGAATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3532  GCCATCAGGGC         
BsaWI         843  ACCGGA               [AT]^CCGG[AT]
             2351  ACCGGA              
BsoBI         872  CTCGGG               C^[CT]CG[GA]G
             3151  CCCGAG              
BspHI        2717  TCATGA               T^CATGA
             3615  TCATGA              
BspLU11I     2363  ACATGT               A^CATGT
             3807  ACATGT              
BstAPI       2033  GCAAAATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2283  GCAGTTAATGC         
BstYI        1329  AGATCC               [GA]^GATC[CT]
             1844  AGATCC              
Bsu36I       1132  CCTGAGG              CC^T[ACGT]AGG
             1204  CCTCAGG             
EaeI          249  TGGCCA               [CT]^GGCC[GA]
             1908  TGGCCA              
Eco81I       1132  CCTGAGG              CC^T[ACGT]AGG
             1204  CCTCAGG             
HapII         844  CCGG                 C^CGG
             2352  CCGG                
HincII       1938  GTTGAC               GT[CT]^[GA]AC
             3987  GTTGAC              
HindII       1938  GTTGAC               GT[CT]^[GA]AC
             3987  GTTGAC              
HpaII         844  CCGG                 C^CGG
             2352  CCGG                
MflI         1329  AGATCC               [GA]^GATC[CT]
             1844  AGATCC              
MluNI         249  TGGCCA               TGG^CCA
             1908  TGGCCA              
MscI          249  TGGCCA               TGG^CCA
             1908  TGGCCA              
MspI          844  CCGG                 C^CGG
             2352  CCGG                
PflMI        1365  CCATATTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1537  CCAGAGGCTGG         
PshAI          82  GACGCTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             4036  GACCAGTGTC          
RcaI         2717  TCATGA               T^CATGA
             3615  TCATGA              
SmlI          986  CTCAAG               C^T[CT][GA]AG
             4598  CTTGAG              
SpeI         1078  ACTAGT               A^CTAGT
             1492  ACTAGT              
SphI          319  GCATGC               GCATG^C
              605  GCATGC              
SspI         1477  AATATT               AAT^ATT
             3391  AATATT              
Van91I       1365  CCATATTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1537  CCAGAGGCTGG         
XhoII        1329  AGATCC               [GA]^GATC[CT]
             1844  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   94 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        AspHI       AvaII       
BanI        BanII       BbvI        BclI        BfaI        
BmyI        BpmI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       Bsp1286I    BspMI       BsrBI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
DsaI        EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        FbaI        Fnu4HI      FokI        HaeII       
HaeIII      HgaI        HhaI        HinP1I      HinfI       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspA1I      MvaI        MwoI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PleI        PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI