##################################
# NO CUT ENZYMES (   52 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        Aor51HI     
AscI        Asp700I     Asp718I     AviII       BamHI       
BbeI        BbvCI       BciVI       BspDI       BspLU11I    
Bst1107I    BstBI       BstZ17I     ClaI        CpoI        
DrdI        Eco47III    EcoRV       FseI        FspI        
HindIII     HpaI        KasI        KpnI        MfeI        
MluI        MunI        NarI        NdeI        NheI        
NotI        NspV        PacI        PaeR7I      PinAI       
Psp1406I    PvuI        RsrII       SalI        SfoI        
SfuI        SnaBI       SpeI        SwaI        XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI         4197  GTCTAC               GT^[AC][GT]AC
AccIII       4811  TCCGGA               T^CCGGA
AflII        6068  CTTAAG               C^TTAAG
Alw44I        736  GTGCAC               G^TGCAC
AocI         4472  CCTCAGG              CC^T[ACGT]AGG
ApaI         2639  GGGCCC               GGGCC^C
ApaLI         736  GTGCAC               G^TGCAC
AseI         6296  ATTAAT               AT^TAAT
AsnI         6296  ATTAAT               AT^TAAT
BbrPI        2188  CACGTG               CAC^GTG
BcgI          368  CGAAAAAGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         6068  CTTAAG               C^TTAAG
BsaBI        3135  GATGTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        4811  TCCGGA               T^CCGGA
BsiWI        1697  CGTACG               C^GTACG
BsmBI        2194  GAGACG               CGTCTC(1/5)
BspEI        4811  TCCGGA               T^CCGGA
BssSI        1144  CACGAG               CACGAG(-5/-1)
Bsu36I       4472  CCTCAGG              CC^T[ACGT]AGG
DraIII       3269  CACAGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         2765  CGGCCG               C^GGCCG
EclXI        2765  CGGCCG               C^GGCCG
Eco52I       2765  CGGCCG               C^GGCCG
Eco81I       4472  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1788  CCTCACCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I      5911  ATGCAT               ATGCA^T
HincII       3508  GTTGAC               GT[CT]^[GA]AC
HindII       3508  GTTGAC               GT[CT]^[GA]AC
MamI         3135  GATGTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         4811  TCCGGA               T^CCGGA
NruI          191  TCGCGA               TCG^CGA
NsiI         5911  ATGCAT               ATGCA^T
PmaCI        2188  CACGTG               CAC^GTG
PmeI         5944  GTTTAAAC             GTTT^AAAC
PmlI         2188  CACGTG               CAC^GTG
PshAI        2992  GACACTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        6296  ATTAAT               AT^TAAT
SbfI         2058  CCTGCAGG             CCTGCA^GG
SexAI        1247  ACCAGGT              A^CC[AT]GGT
SfiI         2766  GGCCGCCTCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI        2394  CGCCGGCG             C[GA]^CCGG[CT]G
SphI         2792  GCATGC               GCATG^C
Sse8387I     2058  CCTGCAGG             CCTGCA^GG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AhdI         1987  GACTACACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3103  GACTGGCTGTC         
AspEI        1987  GACTACACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3103  GACTGGCTGTC         
AspI          724  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3186  GACGGGGTC           
AvrII        2787  CCTAGG               C^CTAGG
             3564  CCTAGG              
BbsI         3872  GAAGAC               GAAGAC(2/6)
             5228  GAAGAC              
BglII        2930  AGATCT               A^GATCT
             4179  AGATCT              
BlnI         2787  CCTAGG               C^CTAGG
             3564  CCTAGG              
BpuAI        3872  GAAGAC               GAAGAC(2/6)
             5228  GAAGAC              
BsaAI        2188  CACGTG               [CT]AC^GT[GA]
             5342  CACGTA              
BsiEI         188  CGGTCG               CG[GA][CT]^CG
             2765  CGGCCG              
BsmI         4360  GAATGC               GAATGC(1/-1)
             5662  GAATGC              
BspHI        4761  TCATGA               T^CATGA
             6133  TCATGA              
DraI         5945  TTTAAA               TTT^AAA
             6535  TTTAAA              
Eam1105I     1987  GACTACACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3103  GACTGGCTGTC         
EcoRI        4170  GAATTC               G^AATTC
             4696  GAATTC              
PflFI         724  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3186  GACGGGGTC           
RcaI         4761  TCATGA               T^CATGA
             6133  TCATGA              
StuI         1710  AGGCCT               AGG^CCT
             2156  AGGCCT              
Tth111I       724  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3186  GACGGGGTC           
XcmI         1241  CCACCAACCAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1937  CCAGCACCACCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  143 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       ApoI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbvI        BclI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BsaHI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    Bsp1407I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssKI       BstAPI      BstEII      BstF5I      
BstNI       BstPI       BstUI       BstXI       BstYI       
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoO65I     EcoRII      EcoT14I     
FauI        FbaI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     KspI        MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NcoI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SacI        SacII       SapI        
Sau3AI      Sau96I      ScaI        ScrFI       SfaNI       
SfcI        SmaI        SmlI        SspBI       SspI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       XmaCI       XmaI