##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I ApaLI AscI
Asp700I Asp718I AviII AvrII BbrPI
BcgI BclI BfrI BlnI BsaBI
BseAI BsiEI BspDI BspEI BspHI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraIII DrdI
EagI EclXI Eco52I EcoO65I EcoRI
FbaI FspI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MroI MunI NdeI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SfiI SfuI SgrAI SnaBI
Sse8387I SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 22 enzymes) #
###################################
AhdI 2918 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 16 AGCGCT AGC^GCT
AseI 901 ATTAAT AT^TAAT
AsnI 901 ATTAAT AT^TAAT
AspEI 2918 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 6038 GAAGAC GAAGAC(2/6)
BpuAI 6038 GAAGAC GAAGAC(2/6)
BsaAI 3538 CACGTA [CT]AC^GT[GA]
BsiWI 1950 CGTACG C^GTACG
BspLU11I 3242 ACATGT A^CATGT
BsrBI 2470 GAGCGG CCGCTC(-3/-3)
BssSI 5529 CACGAG CACGAG(-5/-1)
DraI 4453 TTTAAA TTT^AAA
Eam1105I 2918 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 16 AGCGCT AGC^GCT
EcoRV 4825 GATATC GAT^ATC
MluI 3486 ACGCGT A^CGCGT
PshBI 901 ATTAAT AT^TAAT
SexAI 4385 ACCAGGT A^CC[AT]GGT
SpeI 3558 ACTAGT A^CTAGT
SspI 5999 AATATT AAT^ATT
XbaI 3109 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 578 CGCG CG^CG
3487 CGCG
AcsI 1370 GAATTT [GA]^AATT[CT]
4030 AAATTT
ApoI 1370 GAATTT [GA]^AATT[CT]
4030 AAATTT
AspI 5559 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6041 GACAGTGTC
BsaWI 419 TCCGGT [AT]^CCGG[AT]
2250 TCCGGT
BsmI 1220 GAATGC GAATGC(1/-1)
232 GCATTC
Bsp1407I 5870 TGTACA T^GTACA
6075 TGTACA
BsrFI 2112 GCCGGC [GA]^CCGG[CT]
5315 GCCGGC
BsrGI 5870 TGTACA T^GTACA
6075 TGTACA
BstUI 578 CGCG CG^CG
3487 CGCG
Cfr10I 2112 GCCGGC [GA]^CCGG[CT]
5315 GCCGGC
EcoT22I 3514 ATGCAT ATGCA^T
5990 ATGCAT
FseI 2111 GGCCGGCC GGCCGG^CC
5314 GGCCGGCC
MaeII 366 ACGT A^CGT
3539 ACGT
MvnI 578 CGCG CG^CG
3487 CGCG
NaeI 2112 GCCGGC GCC^GGC
5315 GCCGGC
NgoMIV 2112 GCCGGC G^CCGGC
5315 GCCGGC
NsiI 3514 ATGCAT ATGCA^T
5990 ATGCAT
PflFI 5559 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6041 GACAGTGTC
PshAI 1764 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4171 GACACAAGTC
SapI 1708 GAAGAGC GCTCTTC(1/4)
1777 GAAGAGC
SmaI 1350 CCCGGG CCC^GGG
2394 CCCGGG
SphI 3421 GCATGC GCATG^C
3906 GCATGC
SspBI 5870 TGTACA T^GTACA
6075 TGTACA
Tth111I 5559 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6041 GACAGTGTC
XmaCI 1350 CCCGGG C^CCGGG
2394 CCCGGG
XmaI 1350 CCCGGG C^CCGGG
2394 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbeI BbvCI BbvI BciVI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII