##################################
# NO CUT ENZYMES (   49 enzymes) #
##################################

AclI        AflII       AgeI        Aor51HI     AscI        
AseI        AsnI        AviII       BciVI       BfrI        
BsaBI       BsiWI       BspDI       BspHI       BssHII      
Bst1107I    BstBI       BstZ17I     ClaI        DraI        
Eco47III    EcoRI       EcoRV       EcoT22I     FspI        
HpaI        MamI        MfeI        MluI        MunI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PinAI       PmeI        PshBI       Psp1406I    
PvuI        RcaI        SfuI        SgrAI       SnaBI       
SpeI        SspI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   21 enzymes) #
###################################

AatII        6211  GACGTC               GACGT^C
Acc65I       3385  GGTACC               G^GTACC
AccIII       6217  TCCGGA               T^CCGGA
Asp700I      7719  GAATCATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      3385  GGTACC               G^GTACC
BclI         9762  TGATCA               T^GATCA
BseAI        6217  TCCGGA               T^CCGGA
BspEI        6217  TCCGGA               T^CCGGA
CpoI         4507  CGGACCG              CG^G[AT]CCG
FbaI         9762  TGATCA               T^GATCA
FseI         2235  GGCCGGCC             GGCCGG^CC
KpnI         3385  GGTACC               GGTAC^C
MroI         6217  TCCGGA               T^CCGGA
NdeI         5149  CATATG               CA^TATG
PaeR7I       8742  CTCGAG               C^TCGAG
PshAI        1446  GACCTGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII        4507  CGGACCG              CG^G[AT]CCG
SalI         6208  GTCGAC               G^TCGAC
SphI         3219  GCATGC               GCATG^C
XhoI         8742  CTCGAG               C^TCGAG
XmnI         7719  GAATCATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI         7881  AAATTC               [GA]^AATT[CT]
             9694  GAATTT              
ApoI         7881  AAATTC               [GA]^AATT[CT]
             9694  GAATTT              
AspI         1401  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             9057  GACACTGTC           
BbrPI        1914  CACGTG               CAC^GTG
             3782  CACGTG              
BcgI         8240  CGAGCACCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             9889  GCAGAAGAATCG        
BglII        2593  AGATCT               A^GATCT
             2935  AGATCT              
BsaWI         838  ACCGGA               [AT]^CCGG[AT]
             6217  TCCGGA              
BsiEI        2234  CGGCCG               CG[GA][CT]^CG
             7850  CGGCCG              
EagI         2234  CGGCCG               C^GGCCG
             7850  CGGCCG              
EclXI        2234  CGGCCG               C^GGCCG
             7850  CGGCCG              
Eco52I       2234  CGGCCG               C^GGCCG
             7850  CGGCCG              
HincII       5886  GTCAAC               GT[CT]^[GA]AC
             6208  GTCGAC              
HindII       5886  GTCAAC               GT[CT]^[GA]AC
             6208  GTCGAC              
HindIII       970  AAGCTT               A^AGCTT
             6085  AAGCTT              
PflFI        1401  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             9057  GACACTGTC           
PmaCI        1914  CACGTG               CAC^GTG
             3782  CACGTG              
PmlI         1914  CACGTG               CAC^GTG
             3782  CACGTG              
SapI         9446  GAAGAGC              GCTCTTC(1/4)
             8342  GCTCTTC             
SbfI         1145  CCTGCAGG             CCTGCA^GG
             7532  CCTGCAGG            
ScaI         7417  AGTACT               AGT^ACT
             8869  AGTACT              
SexAI        5881  ACCAGGT              A^CC[AT]GGT
             6111  ACCTGGT             
SmlI         8253  CTCAAG               C^T[CT][GA]AG
             8742  CTCGAG              
Sse8387I     1145  CCTGCAGG             CCTGCA^GG
             7532  CCTGCAGG            
Tth111I      1401  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             9057  GACACTGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  164 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AflIII      AhdI        AluI        Alw44I      AlwI        
AlwNI       AocI        ApaI        ApaLI       AspEI       
AspHI       AvaI        AvaII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbsI        
BbvCI       BbvI        BcnI        BfaI        BglI        
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssKI       
BssSI       BstAPI      BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       BstYI       Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DraIII      DrdI        
DsaI        EaeI        Eam1105I    EarI        Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     KspI        MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        SacII       
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfiI        SfoI        SmaI        SspBI       StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoII       XmaCI       XmaI