##################################
# NO CUT ENZYMES (   58 enzymes) #
##################################

Acc65I      AclI        AgeI        AhdI        AlwNI       
ApaI        AscI        Asp718I     AspEI       AviII       
AvrII       BbeI        BbvCI       BcgI        BglII       
BlnI        BsiEI       BsiWI       BspDI       BspLU11I    
BsrBI       BssHII      BstEII      BstPI       ClaI        
CpoI        DrdI        EagI        Eam1105I    EclXI       
Eco52I      EcoO65I     FseI        FspI        KasI        
KpnI        KspI        MluI        NarI        NheI        
NotI        NruI        PacI        PinAI       PmeI        
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SbfI        SfiI        SfoI        SgrAI       
SphI        Sse8387I    StuI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AatII        2720  GACGTC               GACGT^C
AcyI         2720  GACGTC               G[GA]^CG[CT]C
AflII        5618  CTTAAG               C^TTAAG
AflIII        716  ACACGT               A^C[GA][CT]GT
Aor51HI       629  AGCGCT               AGC^GCT
BbrPI        5779  CACGTG               CAC^GTG
BfrI         5618  CTTAAG               C^TTAAG
BglI         1646  GCCTGTTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         2798  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2798  GCTCAGC              GC^T[ACGT]AGC
BsaHI        2720  GACGTC               G[GA]^CG[CT]C
BseRI        1858  CTCCTC               GAGGAG(10/8)
Bst1107I      479  GTATAC               GTA^TAC
BstAPI       5712  GCAAAGACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        4812  TTCGAA               TT^CGAA
BstZ17I       479  GTATAC               GTA^TAC
CelII        2798  GCTCAGC              GC^T[ACGT]AGC
Eco47III      629  AGCGCT               AGC^GCT
EcoNI        5760  CCTGCACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1428  GAATTC               G^AATTC
EcoRV        4359  GATATC               GAT^ATC
Hin1I        2720  GACGTC               G[GA]^CG[CT]C
NaeI         2869  GCCGGC               GCC^GGC
NgoMIV       2869  GCCGGC               G^CCGGC
NspV         4812  TTCGAA               TT^CGAA
PaeR7I        822  CTCGAG               C^TCGAG
PmaCI        5779  CACGTG               CAC^GTG
PmlI         5779  CACGTG               CAC^GTG
PshAI        3835  GACTTGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI        4472  ACCTGGT              A^CC[AT]GGT
SfuI         4812  TTCGAA               TT^CGAA
SmaI          684  CCCGGG               CCC^GGG
SpeI         7301  ACTAGT               A^CTAGT
SwaI         5157  ATTTAAAT             ATTT^AAAT
XhoI          822  CTCGAG               C^TCGAG
XmaCI         684  CCCGGG               C^CCGGG
XmaI          684  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccIII       3584  TCCGGA               T^CCGGA
             6640  TCCGGA              
Alw44I       1392  GTGCAC               G^TGCAC
             3167  GTGCAC              
ApaLI        1392  GTGCAC               G^TGCAC
             3167  GTGCAC              
AspI         2717  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5631  GACAGGGTC           
AvaI          684  CCCGGG               C^[CT]CG[GA]G
              822  CTCGAG              
BamHI        1303  GGATCC               G^GATCC
             2395  GGATCC              
BanI         1794  GGCACC               G^G[CT][GA]CC
             2898  GGCACC              
BciVI        3428  GGATAC               GTATCC(6/5)
             6295  GTATCC              
BsaI          639  GAGACC               GGTCTC(1/5)
             7433  GAGACC              
BsaWI        3584  TCCGGA               [AT]^CCGG[AT]
             6640  TCCGGA              
BseAI        3584  TCCGGA               T^CCGGA
             6640  TCCGGA              
BsmBI        2951  CGTCTC               CGTCTC(1/5)
             5736  CGTCTC              
BsoBI         684  CCCGGG               C^[CT]CG[GA]G
              822  CTCGAG              
BspEI        3584  TCCGGA               T^CCGGA
             6640  TCCGGA              
BspHI        6126  TCATGA               T^CATGA
             6786  TCATGA              
BsrFI        2869  GCCGGC               [GA]^CCGG[CT]
             3679  GCCGGT              
Cfr10I       2869  GCCGGC               [GA]^CCGG[CT]
             3679  GCCGGT              
DsaI         1154  CCATGG               C^C[GA][CT]GG
             5984  CCATGG              
EcoT22I      3212  ATGCAT               ATGCA^T
             6245  ATGCAT              
FauI         2126  GCGGG                CCCGC(4/6)
             2754  GCGGG               
HaeII         629  AGCGCT               [GA]GCGC^[CT]
             2833  GGCGCT              
MfeI         1831  CAATTG               C^AATTG
             4563  CAATTG              
MroI         3584  TCCGGA               T^CCGGA
             6640  TCCGGA              
MunI         1831  CAATTG               C^AATTG
             4563  CAATTG              
NcoI         1154  CCATGG               C^CATGG
             5984  CCATGG              
NdeI         2817  CATATG               CA^TATG
             7352  CATATG              
NsiI         3212  ATGCAT               ATGCA^T
             6245  ATGCAT              
PflFI        2717  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5631  GACAGGGTC           
RcaI         6126  TCATGA               T^CATGA
             6786  TCATGA              
SapI         1530  GAAGAGC              GCTCTTC(1/4)
             7119  GAAGAGC             
ScaI         3361  AGTACT               AGT^ACT
             4392  AGTACT              
SnaBI        1837  TACGTA               TAC^GTA
             4368  TACGTA              
Tth111I      2717  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5631  GACAGGGTC           
XbaI         3957  TCTAGA               T^CTAGA
             4691  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  129 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AluI        AlwI        AocI        ApoI        AseI        
AsnI        Asp700I     AspHI       AvaII       BalI        
BanII       BbsI        BbvI        BclI        BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaAI       
BsaBI       BsaJI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
Bsp1407I    BspMI       BsrDI       BsrGI       BsrI        
BssKI       BssSI       BstF5I      BstNI       BstUI       
BstXI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
DraIII      EaeI        EarI        Eco81I      EcoO109I    
EcoRII      EcoT14I     FbaI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HincII      HindII      HindIII     HinfI       HpaI        
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MamI        MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PshBI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SspBI       
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII       XmnI