##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI Aor51HI AscI Asp718I
AspEI AviII AvrII BbeI BcgI
BlnI BsaBI BseAI BsiEI BsiWI
BspDI BspEI BssHII BssSI Bst1107I
BstBI BstZ17I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FseI FspI HpaI
KasI KpnI KspI MamI MfeI
MroI MunI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PinAI PmeI PshAI Psp1406I
PvuI RsrII SacII SalI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 2517 CGCG CG^CG
AcyI 2722 GACGCC G[GA]^CG[CT]C
AflII 1170 CTTAAG C^TTAAG
Alw44I 549 GTGCAC G^TGCAC
ApaI 4766 GGGCCC GGGCC^C
ApaLI 549 GTGCAC G^TGCAC
AseI 4509 ATTAAT AT^TAAT
AsnI 4509 ATTAAT AT^TAAT
BamHI 2435 GGATCC G^GATCC
BclI 3757 TGATCA T^GATCA
BfrI 1170 CTTAAG C^TTAAG
BsaHI 2722 GACGCC G[GA]^CG[CT]C
BsaWI 312 TCCGGT [AT]^CCGG[AT]
Bsp1407I 55 TGTACA T^GTACA
BspHI 1879 TCATGA T^CATGA
BspLU11I 4014 ACATGT A^CATGT
BsrBI 764 GAGCGG CCGCTC(-3/-3)
BsrFI 192 ACCGGC [GA]^CCGG[CT]
BsrGI 55 TGTACA T^GTACA
BstEII 4025 GGTAACC G^GT[ACGT]ACC
BstPI 4025 GGTAACC G^GT[ACGT]ACC
BstUI 2517 CGCG CG^CG
Cfr10I 192 ACCGGC [GA]^CCGG[CT]
DrdI 4290 GACCTGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4025 GGTAACC G^GT[ACGT]ACC
FbaI 3757 TGATCA T^GATCA
Hin1I 2722 GACGCC G[GA]^CG[CT]C
HincII 623 GTTGAC GT[CT]^[GA]AC
HindII 623 GTTGAC GT[CT]^[GA]AC
HindIII 3655 AAGCTT A^AGCTT
MluI 2516 ACGCGT A^CGCGT
MvnI 2517 CGCG CG^CG
NheI 3910 GCTAGC G^CTAGC
PshBI 4509 ATTAAT AT^TAAT
RcaI 1879 TCATGA T^CATGA
SbfI 2631 CCTGCAGG CCTGCA^GG
SexAI 2239 ACCTGGT A^CC[AT]GGT
SmaI 629 CCCGGG CCC^GGG
SphI 4690 GCATGC GCATG^C
Sse8387I 2631 CCTGCAGG CCTGCA^GG
SspBI 55 TGTACA T^GTACA
SspI 1552 AATATT AAT^ATT
XmaCI 629 CCCGGG C^CCGGG
XmaI 629 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
Asp700I 1518 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4078 GAAAGATTTC
BbrPI 461 CACGTG CAC^GTG
2526 CACGTG
BciVI 423 GTATCC GTATCC(6/5)
2520 GTATCC
BglI 518 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1626 GCCTGCTAGGC
BglII 434 AGATCT A^GATCT
4307 AGATCT
BsmBI 2448 CGTCTC CGTCTC(1/5)
664 GAGACG
BsmI 1758 GAATGC GAATGC(1/-1)
4411 GCATTC
NcoI 2840 CCATGG C^CATGG
4760 CCATGG
PaeR7I 2923 CTCGAG C^TCGAG
3093 CTCGAG
PmaCI 461 CACGTG CAC^GTG
2526 CACGTG
PmlI 461 CACGTG CAC^GTG
2526 CACGTG
PvuII 2548 CAGCTG CAG^CTG
4827 CAGCTG
StuI 1745 AGGCCT AGG^CCT
4242 AGGCCT
XhoI 2923 CTCGAG C^TCGAG
3093 CTCGAG
XmnI 1518 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4078 GAAAGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI AspHI
AspI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII