##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

AatII       AccII       AccIII      AcyI        AgeI        
Alw44I      Aor51HI     ApaLI       AscI        AviII       
AvrII       BalI        BamHI       BbeI        BbrPI       
BcgI        BciVI       BlnI        BsaHI       BsaWI       
BseAI       BsiEI       BsmBI       BspDI       BspEI       
BsrBI       BssHII      BstEII      BstPI       BstUI       
ClaI        CpoI        DraIII      DsaI        EaeI        
EagI        EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRV       FseI        FspI        Hin1I       
KasI        KspI        MluI        MluNI       MroI        
MscI        MvnI        NarI        NcoI        NotI        
NruI        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PvuI        
RsrII       SacI        SacII       SalI        SbfI        
SfiI        SfoI        SgrAI       SnaBI       Sse8387I    
StuI        Van91I      XhoI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AhdI         4231  GACTGTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI         5832  GGGCCC               GGGCC^C
AspEI        4231  GACTGTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        3785  GAGCAC               G[AT]GC[AT]^C
AspI         5510  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI        5437  GCTGAGG              CCTCAGC(-5/-2)
BclI         3670  TGATCA               T^GATCA
BglI         2870  GCCGTAAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         4891  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     4891  GCTCAGC              GC^T[ACGT]AGC
BsaAI        5061  CACGTA               [CT]AC^GT[GA]
BsaBI        4965  GATGGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI      3785  GAGCAC               G[AT]GC[AT]^C
BsiWI        3818  CGTACG               C^GTACG
BsmFI          81  GGGAC                GGGAC(10/14)
Bsp1407I     5124  TGTACA               T^GTACA
BspMI        1175  GCAGGT               ACCTGC(4/8)
BsrFI          15  GCCGGC               [GA]^CCGG[CT]
BsrGI        5124  TGTACA               T^GTACA
BssSI        3782  CACGAG               CACGAG(-5/-1)
BstAPI       4700  GCAACCAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII        4891  GCTCAGC              GC^T[ACGT]AGC
CfoI         3987  GCGC                 GCG^C
Cfr10I         15  GCCGGC               [GA]^CCGG[CT]
Eam1105I     4231  GACTGTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI         3670  TGATCA               T^GATCA
HaeII        3986  GGCGCT               [GA]GCGC^[CT]
HgaI          566  GACGC                GACGC(5/10)
HhaI         3987  GCGC                 GCG^C
HinP1I       3987  GCGC                 G^CGC
HincII       3475  GTTAAC               GT[CT]^[GA]AC
HindII       3475  GTTAAC               GT[CT]^[GA]AC
HpaI         3475  GTTAAC               GTT^AAC
MamI         4965  GATGGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         3617  CAATTG               C^AATTG
MunI         3617  CAATTG               C^AATTG
NaeI           15  GCCGGC               GCC^GGC
NdeI         6144  CATATG               CA^TATG
NgoMIV         15  GCCGGC               G^CCGGC
NheI         1171  GCTAGC               G^CTAGC
PflFI        5510  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
ScaI         5314  AGTACT               AGT^ACT
SexAI        4840  ACCAGGT              A^CC[AT]GGT
SmaI         4482  CCCGGG               CCC^GGG
SpeI         6439  ACTAGT               A^CTAGT
SspBI        5124  TGTACA               T^GTACA
Tth111I      5510  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        4482  CCCGGG               C^CCGGG
XmaI         4482  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

Acc65I       1609  GGTACC               G^GTACC
             5347  GGTACC              
AclI         3447  AACGTT               AA^CGTT
             5548  AACGTT              
AflIII       2612  ACATGT               A^C[GA][CT]GT
             5655  ACATGT              
AlwNI         207  CAGAGACTG            CAG[ACGT][ACGT][ACGT]^CTG
             3663  CAGAAACTG           
AocI         5289  CCTCAGG              CC^T[ACGT]AGG
             6107  CCTTAGG             
Asp718I      1609  GGTACC               G^GTACC
             5347  GGTACC              
AvaI         4482  CCCGGG               C^[CT]CG[GA]G
             5493  CTCGGG              
BanI         1609  GGTACC               G^G[CT][GA]CC
             5347  GGTACC              
BanII        5790  GAGCCC               G[GA]GC[CT]^C
             5832  GGGCCC              
BglII        2980  AGATCT               A^GATCT
             3157  AGATCT              
BsaI         2909  GAGACC               GGTCTC(1/5)
             5508  GAGACC              
BsgI         5482  CTGCAC               GTGCAG(16/14)
             4662  GTGCAG              
BsmI         2545  GAATGC               GAATGC(1/-1)
             2825  GAATGC              
BsoBI        4482  CCCGGG               C^[CT]CG[GA]G
             5493  CTCGGG              
BspHI        1081  TCATGA               T^CATGA
             1498  TCATGA              
BspLU11I     2612  ACATGT               A^CATGT
             5655  ACATGT              
Bst1107I     1658  GTATAC               GTA^TAC
             5759  GTATAC              
BstBI        1463  TTCGAA               TT^CGAA
             4266  TTCGAA              
BstZ17I      1658  GTATAC               GTA^TAC
             5759  GTATAC              
Bsu36I       5289  CCTCAGG              CC^T[ACGT]AGG
             6107  CCTTAGG             
DrdI         1276  GACATTTTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3178  GACATTACAGTC        
Eco81I       5289  CCTCAGG              CC^T[ACGT]AGG
             6107  CCTTAGG             
EcoRI        3905  GAATTC               G^AATTC
             5264  GAATTC              
EcoT14I      2316  CCTTGG               C^C[AT][AT]GG
             3876  CCAAGG              
EcoT22I      5232  ATGCAT               ATGCA^T
             6461  ATGCAT              
KpnI         1609  GGTACC               GGTAC^C
             5347  GGTACC              
NsiI         5232  ATGCAT               ATGCA^T
             6461  ATGCAT              
NspV         1463  TTCGAA               TT^CGAA
             4266  TTCGAA              
PpuMI        4260  AGGTCCT              [GA]G^G[AT]CC[CT]
             5293  AGGACCC             
Psp1406I     3447  AACGTT               AA^CGTT
             5548  AACGTT              
RcaI         1081  TCATGA               T^CATGA
             1498  TCATGA              
SfuI         1463  TTCGAA               TT^CGAA
             4266  TTCGAA              
StyI         2316  CCTTGG               C^C[AT][AT]GG
             3876  CCAAGG              
SwaI         2169  ATTTAAAT             ATTT^AAAT
             6557  ATTTAAAT            
XbaI         2533  TCTAGA               T^CTAGA
             4462  TCTAGA              
XcmI         3633  CCAAAAAGGAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4620  CCAAAGAAGAATTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflII       
AluI        AlwI        ApoI        AseI        AsnI        
Asp700I     AvaII       BbsI        BbvI        BcnI        
BfaI        BfrI        BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsiYI       BslI        BsmAI       
Bsp1286I    BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstXI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraI        DraII       EarI        
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PleI        PshBI       PstI        PvuII       
RsaI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SphI        SspI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       XmnI