##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AclI AgeI AhdI Alw44I
AocI ApaI ApaLI AscI AspEI
AspI AviII BamHI BglI BseRI
BsiWI Bsp1407I BsrFI BsrGI BstBI
Bsu36I Cfr10I CpoI Eam1105I Eco81I
FseI FspI HgaI MluI NaeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
Psp1406I PvuI RsrII SalI SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SwaI Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 6022 GGTACC G^GTACC
Aor51HI 19 AGCGCT AGC^GCT
Asp718I 6022 GGTACC G^GTACC
AvrII 6053 CCTAGG C^CTAGG
BbrPI 2857 CACGTG CAC^GTG
BcgI 1513 GCAGGCGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 691 GGATAC GTATCC(6/5)
BlnI 6053 CCTAGG C^CTAGG
BlpI 4394 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4394 GCTCAGC GC^T[ACGT]AGC
BsaBI 5421 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 5245 CGGCCG CG[GA][CT]^CG
BsmBI 1001 GAGACG CGTCTC(1/5)
BspLU11I 2277 ACATGT A^CATGT
BsrBI 1165 CCGCTC CCGCTC(-3/-3)
BstXI 5354 CCAGAGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 4394 GCTCAGC GC^T[ACGT]AGC
EagI 5245 CGGCCG C^GGCCG
EclXI 5245 CGGCCG C^GGCCG
Eco47III 19 AGCGCT AGC^GCT
Eco52I 5245 CGGCCG C^GGCCG
EcoNI 5752 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 268 GAATTC G^AATTC
HindIII 4612 AAGCTT A^AGCTT
HpaI 2219 GTTAAC GTT^AAC
KpnI 6022 GGTACC GGTAC^C
KspI 65 CCGCGG CCGC^GG
MamI 5421 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4993 CAATTG C^AATTG
MunI 4993 CAATTG C^AATTG
NdeI 2195 CATATG CA^TATG
PmaCI 2857 CACGTG CAC^GTG
PmlI 2857 CACGTG CAC^GTG
PshAI 2274 GACACATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1722 GAGCTC GAGCT^C
SacII 65 CCGCGG CCGC^GG
SspI 2648 AATATT AAT^ATT
StuI 4470 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 789 TCCGGA T^CCGGA
5634 TCCGGA
AcyI 47 GGCGCC G[GA]^CG[CT]C
1516 GGCGCC
AflII 1618 CTTAAG C^TTAAG
5473 CTTAAG
AflIII 2277 ACATGT A^C[GA][CT]GT
2858 ACGTGT
AseI 4178 ATTAAT AT^TAAT
5808 ATTAAT
AsnI 4178 ATTAAT AT^TAAT
5808 ATTAAT
BbeI 47 GGCGCC GGCGC^C
1516 GGCGCC
BbvCI 5752 CCTCAGC CCTCAGC(-5/-2)
3361 GCTGAGG
BfrI 1618 CTTAAG C^TTAAG
5473 CTTAAG
BsaAI 719 TACGTG [CT]AC^GT[GA]
2857 CACGTG
BsaHI 47 GGCGCC G[GA]^CG[CT]C
1516 GGCGCC
BsaI 2968 GAGACC GGTCTC(1/5)
949 GGTCTC
BsaWI 789 TCCGGA [AT]^CCGG[AT]
5634 TCCGGA
BseAI 789 TCCGGA T^CCGGA
5634 TCCGGA
BspDI 845 ATCGAT AT^CGAT
1133 ATCGAT
BspEI 789 TCCGGA T^CCGGA
5634 TCCGGA
BssHII 72 GCGCGC G^CGCGC
92 GCGCGC
BssSI 1094 CACGAG CACGAG(-5/-1)
5222 CACGAG
Bst1107I 6134 GTATAC GTA^TAC
6166 GTATAC
BstAPI 1814 GCATGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5344 GCATGGGATGC
BstZ17I 6134 GTATAC GTA^TAC
6166 GTATAC
ClaI 845 ATCGAT AT^CGAT
1133 ATCGAT
DraIII 4153 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
4378 CACTGGGTG
DrdI 941 GACATAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1316 GACTCCCGGGTC
EcoT22I 4917 ATGCAT ATGCA^T
5948 ATGCAT
Hin1I 47 GGCGCC G[GA]^CG[CT]C
1516 GGCGCC
HincII 1613 GTTGAC GT[CT]^[GA]AC
2219 GTTAAC
HindII 1613 GTTGAC GT[CT]^[GA]AC
2219 GTTAAC
KasI 47 GGCGCC G^GCGCC
1516 GGCGCC
MroI 789 TCCGGA T^CCGGA
5634 TCCGGA
NarI 47 GGCGCC GG^CGCC
1516 GGCGCC
NsiI 4917 ATGCAT ATGCA^T
5948 ATGCAT
PshBI 4178 ATTAAT AT^TAAT
5808 ATTAAT
ScaI 2812 AGTACT AGT^ACT
4639 AGTACT
SfoI 47 GGCGCC GGC^GCC
1516 GGCGCC
SmaI 1320 CCCGGG CCC^GGG
1374 CCCGGG
SphI 4998 GCATGC GCATG^C
5451 GCATGC
XbaI 1898 TCTAGA T^CTAGA
2282 TCTAGA
XcmI 874 CCAGCTCTTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2839 CCAGGACCCACATGG
XmaCI 1320 CCCGGG C^CCGGG
1374 CCCGGG
XmaI 1320 CCCGGG C^CCGGG
1374 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BclI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspHI BspMI BsrDI BsrI BssKI
BstEII BstF5I BstNI BstPI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoO65I EcoRII EcoRV
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI