################################## # NO CUT ENZYMES ( 56 enzymes) # ################################## AatII AclI AgeI AhdI Alw44I AocI ApaI ApaLI AscI AspEI AspI AviII BamHI BglI BseRI BsiWI Bsp1407I BsrFI BsrGI BstBI Bsu36I Cfr10I CpoI Eam1105I Eco81I FseI FspI HgaI MluI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI Psp1406I PvuI RsrII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### Acc65I 6022 GGTACC G^GTACC Aor51HI 19 AGCGCT AGC^GCT Asp718I 6022 GGTACC G^GTACC AvrII 6053 CCTAGG C^CTAGG BbrPI 2857 CACGTG CAC^GTG BcgI 1513 GCAGGCGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 691 GGATAC GTATCC(6/5) BlnI 6053 CCTAGG C^CTAGG BlpI 4394 GCTCAGC GC^T[ACGT]AGC Bpu1102I 4394 GCTCAGC GC^T[ACGT]AGC BsaBI 5421 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 5245 CGGCCG CG[GA][CT]^CG BsmBI 1001 GAGACG CGTCTC(1/5) BspLU11I 2277 ACATGT A^CATGT BsrBI 1165 CCGCTC CCGCTC(-3/-3) BstXI 5354 CCAGAGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 4394 GCTCAGC GC^T[ACGT]AGC EagI 5245 CGGCCG C^GGCCG EclXI 5245 CGGCCG C^GGCCG Eco47III 19 AGCGCT AGC^GCT Eco52I 5245 CGGCCG C^GGCCG EcoNI 5752 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 268 GAATTC G^AATTC HindIII 4612 AAGCTT A^AGCTT HpaI 2219 GTTAAC GTT^AAC KpnI 6022 GGTACC GGTAC^C KspI 65 CCGCGG CCGC^GG MamI 5421 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 4993 CAATTG C^AATTG MunI 4993 CAATTG C^AATTG NdeI 2195 CATATG CA^TATG PmaCI 2857 CACGTG CAC^GTG PmlI 2857 CACGTG CAC^GTG PshAI 2274 GACACATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 1722 GAGCTC GAGCT^C SacII 65 CCGCGG CCGC^GG SspI 2648 AATATT AAT^ATT StuI 4470 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 41 enzymes) # ################################### AccIII 789 TCCGGA T^CCGGA 5634 TCCGGA AcyI 47 GGCGCC G[GA]^CG[CT]C 1516 GGCGCC AflII 1618 CTTAAG C^TTAAG 5473 CTTAAG AflIII 2277 ACATGT A^C[GA][CT]GT 2858 ACGTGT AseI 4178 ATTAAT AT^TAAT 5808 ATTAAT AsnI 4178 ATTAAT AT^TAAT 5808 ATTAAT BbeI 47 GGCGCC GGCGC^C 1516 GGCGCC BbvCI 5752 CCTCAGC CCTCAGC(-5/-2) 3361 GCTGAGG BfrI 1618 CTTAAG C^TTAAG 5473 CTTAAG BsaAI 719 TACGTG [CT]AC^GT[GA] 2857 CACGTG BsaHI 47 GGCGCC G[GA]^CG[CT]C 1516 GGCGCC BsaI 2968 GAGACC GGTCTC(1/5) 949 GGTCTC BsaWI 789 TCCGGA [AT]^CCGG[AT] 5634 TCCGGA BseAI 789 TCCGGA T^CCGGA 5634 TCCGGA BspDI 845 ATCGAT AT^CGAT 1133 ATCGAT BspEI 789 TCCGGA T^CCGGA 5634 TCCGGA BssHII 72 GCGCGC G^CGCGC 92 GCGCGC BssSI 1094 CACGAG CACGAG(-5/-1) 5222 CACGAG Bst1107I 6134 GTATAC GTA^TAC 6166 GTATAC BstAPI 1814 GCATGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5344 GCATGGGATGC BstZ17I 6134 GTATAC GTA^TAC 6166 GTATAC ClaI 845 ATCGAT AT^CGAT 1133 ATCGAT DraIII 4153 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG 4378 CACTGGGTG DrdI 941 GACATAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1316 GACTCCCGGGTC EcoT22I 4917 ATGCAT ATGCA^T 5948 ATGCAT Hin1I 47 GGCGCC G[GA]^CG[CT]C 1516 GGCGCC HincII 1613 GTTGAC GT[CT]^[GA]AC 2219 GTTAAC HindII 1613 GTTGAC GT[CT]^[GA]AC 2219 GTTAAC KasI 47 GGCGCC G^GCGCC 1516 GGCGCC MroI 789 TCCGGA T^CCGGA 5634 TCCGGA NarI 47 GGCGCC GG^CGCC 1516 GGCGCC NsiI 4917 ATGCAT ATGCA^T 5948 ATGCAT PshBI 4178 ATTAAT AT^TAAT 5808 ATTAAT ScaI 2812 AGTACT AGT^ACT 4639 AGTACT SfoI 47 GGCGCC GGC^GCC 1516 GGCGCC SmaI 1320 CCCGGG CCC^GGG 1374 CCCGGG SphI 4998 GCATGC GCATG^C 5451 GCATGC XbaI 1898 TCTAGA T^CTAGA 2282 TCTAGA XcmI 874 CCAGCTCTTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2839 CCAGGACCCACATGG XmaCI 1320 CCCGGG C^CCGGG 1374 CCCGGG XmaI 1320 CCCGGG C^CCGGG 1374 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BclI BcnI BfaI BglII BmyI BpmI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspMI BsrDI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoO65I EcoRII EcoRV EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI