##################################
# NO CUT ENZYMES (   71 enzymes) #
##################################

Acc65I      AccIII      AflIII      AgeI        AhdI        
AscI        Asp700I     Asp718I     AspEI       AvrII       
BamHI       BbeI        BbrPI       BbvCI       BciVI       
BclI        BglI        BglII       BlnI        BsaAI       
BsaWI       BseAI       BsiWI       BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BssHII      Bst1107I    
BstBI       BstZ17I     ClaI        CpoI        DrdI        
Eam1105I    EcoRV       FbaI        FseI        HindIII     
KasI        KpnI        MluI        MroI        NaeI        
NarI        NdeI        NgoMIV      NruI        NspV        
PacI        PinAI       PmaCI       PmeI        PmlI        
PshAI       PvuI        RcaI        RsrII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SnaBI       Sse8387I    SwaI        XbaI        
XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccII         512  CGCG                 CG^CG
AclI         1804  AACGTT               AA^CGTT
AflII        2590  CTTAAG               C^TTAAG
Alw44I        942  GTGCAC               G^TGCAC
Aor51HI      2594  AGCGCT               AGC^GCT
ApaLI         942  GTGCAC               G^TGCAC
AseI         2424  ATTAAT               AT^TAAT
AsnI         2424  ATTAAT               AT^TAAT
BcgI         2513  CGATACACTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         2590  CTTAAG               C^TTAAG
BsaBI        3834  GATTGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          634  GAGACC               GGTCTC(1/5)
BsiEI           2  CGGCCG               CG[GA][CT]^CG
BsmBI        3134  CGTCTC               CGTCTC(1/5)
BsrBI         347  CCGCTC               CCGCTC(-3/-3)
BstUI         512  CGCG                 CG^CG
BstXI        1865  CCAAAATTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        4002  GGATCT               [GA]^GATC[CT]
DraIII       2019  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI            2  CGGCCG               C^GGCCG
EclXI           2  CGGCCG               C^GGCCG
Eco47III     2594  AGCGCT               AGC^GCT
Eco52I          2  CGGCCG               C^GGCCG
EcoNI        3976  CCTGCCACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HpaI         3384  GTTAAC               GTT^AAC
KspI          511  CCGCGG               CCGC^GG
MamI         3834  GATTGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI         4002  GGATCT               [GA]^GATC[CT]
MvnI          512  CGCG                 CG^CG
NheI         3879  GCTAGC               G^CTAGC
NotI            1  GCGGCCGC             GC^GGCCGC
PaeR7I        585  CTCGAG               C^TCGAG
PflMI        3240  CCAAACTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        2424  ATTAAT               AT^TAAT
Psp1406I     1804  AACGTT               AA^CGTT
SacI          430  GAGCTC               GAGCT^C
SacII         511  CCGCGG               CCGC^GG
SapI         1792  GAAGAGC              GCTCTTC(1/4)
SgrAI         935  CGCCGGTG             C[GA]^CCGG[CT]G
SpeI         3523  ACTAGT               A^CTAGT
SspI         3439  AATATT               AAT^ATT
Van91I       3240  CCAAACTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          585  CTCGAG               C^TCGAG
XhoII        4002  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AatII        2800  GACGTC               GACGT^C
             3132  GACGTC              
AspI          178  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3200  GACAGTGTC           
AviII        2110  TGCGCA               TGC^GCA
             2748  TGCGCA              
BsgI         1193  CTGCAC               GTGCAG(16/14)
             1433  CTGCAC              
BsmI         2575  GCATTC               GAATGC(1/-1)
             4097  GCATTC              
Bsp1407I     2356  TGTACA               T^GTACA
             3373  TGTACA              
BsrFI         321  GCCGGT               [GA]^CCGG[CT]
              936  GCCGGT              
BsrGI        2356  TGTACA               T^GTACA
             3373  TGTACA              
BssSI        3295  CACGAG               CACGAG(-5/-1)
              163  CTCGTG              
BstEII        157  GGTGACC              G^GT[ACGT]ACC
             2528  GGTCACC             
BstPI         157  GGTGACC              G^GT[ACGT]ACC
             2528  GGTCACC             
Cfr10I        321  GCCGGT               [GA]^CCGG[CT]
              936  GCCGGT              
DraI         3023  TTTAAA               TTT^AAA
             3351  TTTAAA              
EcoO65I       157  GGTGACC              G^GT[ACGT]ACC
             2528  GGTCACC             
EcoRI         874  GAATTC               G^AATTC
             2465  GAATTC              
EcoT22I      1885  ATGCAT               ATGCA^T
             3419  ATGCAT              
FspI         2110  TGCGCA               TGC^GCA
             2748  TGCGCA              
HaeII         742  AGCGCC               [GA]GCGC^[CT]
             2594  AGCGCT              
HincII        184  GTCAAC               GT[CT]^[GA]AC
             3384  GTTAAC              
HindII        184  GTCAAC               GT[CT]^[GA]AC
             3384  GTTAAC              
MfeI         2835  CAATTG               C^AATTG
             3377  CAATTG              
MunI         2835  CAATTG               C^AATTG
             3377  CAATTG              
NcoI         1715  CCATGG               C^CATGG
             2028  CCATGG              
NsiI         1885  ATGCAT               ATGCA^T
             3419  ATGCAT              
PflFI         178  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3200  GACAGTGTC           
SphI          930  GCATGC               GCATG^C
             1887  GCATGC              
SspBI        2356  TGTACA               T^GTACA
             3373  TGTACA              
TaqI          586  TCGA                 T^CGA
             2069  TCGA                
Tth111I       178  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3200  GACAGTGTC           
TthHB8I       586  TCGA                 T^CGA
             2069  TCGA                
XcmI          197  CCATGACCCACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3240  CCAAACTCTGGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AccI        AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       AocI        ApaI        
ApoI        AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbsI        BbvI        BcnI        
BfaI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaHI       BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       Bsu36I      Cac8I       CelII       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        Eco81I      EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        SmlI        StuI        StyI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
XmaCI       XmaI