##################################
# NO CUT ENZYMES (   79 enzymes) #
##################################

AatII       Acc65I      AcyI        AhdI        AocI        
Aor51HI     ApaI        AscI        Asp718I     AspEI       
AvaI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BcnI        BglI        BpmI        BsaHI       
BsiWI       BsoBI       BspMI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CpoI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     FseI        Hin1I       HpaI        KasI        
KpnI        KspI        MfeI        MluI        MunI        
NaeI        NarI        NciI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PmaCI       PmlI        PshAI       PvuI        RsrII       
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SmaI        SnaBI       Sse8387I    
SwaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccI         2820  GTAGAC               GT^[AC][GT]AC
AccII         736  CGCG                 CG^CG
AccIII       1133  TCCGGA               T^CCGGA
AclI         5753  AACGTT               AA^CGTT
AgeI         3502  ACCGGT               A^CCGGT
AviII        1150  TGCGCA               TGC^GCA
AvrII        6591  CCTAGG               C^CTAGG
BalI         3922  TGGCCA               TGG^CCA
BamHI        5071  GGATCC               G^GATCC
BanI         2084  GGTGCC               G^G[CT][GA]CC
BlnI         6591  CCTAGG               C^CTAGG
BsaI         3054  GGTCTC               GGTCTC(1/5)
BseAI        1133  TCCGGA               T^CCGGA
BsgI         6421  GTGCAG               GTGCAG(16/14)
BsiEI        4840  CGGTCG               CG[GA][CT]^CG
BsmBI        5733  CGTCTC               CGTCTC(1/5)
BspDI        2159  ATCGAT               AT^CGAT
BspEI        1133  TCCGGA               T^CCGGA
BsrBI        1163  GAGCGG               CCGCTC(-3/-3)
BsrFI        3502  ACCGGT               [GA]^CCGG[CT]
BstUI         736  CGCG                 CG^CG
Cfr10I       3502  ACCGGT               [GA]^CCGG[CT]
ClaI         2159  ATCGAT               AT^CGAT
DraII        3638  GGGACCT              [GA]G^G[ACGT]CC[CT]
EaeI         3922  TGGCCA               [CT]^GGCC[GA]
EcoO109I     3638  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRV        3715  GATATC               GAT^ATC
FspI         1150  TGCGCA               TGC^GCA
HaeII        5847  GGCGCT               [GA]GCGC^[CT]
MluNI        3922  TGGCCA               TGG^CCA
MroI         1133  TCCGGA               T^CCGGA
MscI         3922  TGGCCA               TGG^CCA
MspA1I        333  CAGCTG               C[AC]G^C[GT]G
MvnI          736  CGCG                 CG^CG
PinAI        3502  ACCGGT               A^CCGGT
PmeI         4018  GTTTAAAC             GTTT^AAAC
PpuMI        3638  GGGACCT              [GA]G^G[AT]CC[CT]
Psp1406I     5753  AACGTT               AA^CGTT
PstI         1439  CTGCAG               CTGCA^G
PvuII         333  CAGCTG               CAG^CTG
ScaI         2493  AGTACT               AGT^ACT
SgrAI        3501  CACCGGTG             C[GA]^CCGG[CT]G
SphI         4611  GCATGC               GCATG^C
StuI         5151  AGGCCT               AGG^CCT
XbaI         2996  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   41 enzymes) #
###################################

AflII        1598  CTTAAG               C^TTAAG
             3599  CTTAAG              
AlwNI         333  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              705  CAGATTCTG           
AseI          839  ATTAAT               AT^TAAT
             4678  ATTAAT              
AsnI          839  ATTAAT               AT^TAAT
             4678  ATTAAT              
AspI         1228  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2473  GACTGAGTC           
BclI         2001  TGATCA               T^GATCA
             5727  TGATCA              
BfrI         1598  CTTAAG               C^TTAAG
             3599  CTTAAG              
BlpI          272  GCTAAGC              GC^T[ACGT]AGC
             4518  GCTGAGC             
Bpu1102I      272  GCTAAGC              GC^T[ACGT]AGC
             4518  GCTGAGC             
BsaAI        1314  TACGTG               [CT]AC^GT[GA]
             5533  TACGTG              
BsaWI        1133  TCCGGA               [AT]^CCGG[AT]
             3502  ACCGGT              
BspHI        3228  TCATGA               T^CATGA
             5267  TCATGA              
BsrDI        4788  CATTGC               GCAATG(2/0)
             1673  GCAATG              
BstAPI       4615  GCAAATATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             4942  GCAAGGGATGC         
CelII         272  GCTAAGC              GC^T[ACGT]AGC
             4518  GCTGAGC             
DraIII       6271  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             6273  CACAGTGTG           
DsaI         4492  CCATGG               C^C[GA][CT]GG
             6144  CCATGG              
EcoRI        2136  GAATTC               G^AATTC
             3930  GAATTC              
EcoT22I        93  ATGCAT               ATGCA^T
             3214  ATGCAT              
FauI         1636  GCGGG                CCCGC(4/6)
             4980  GCGGG               
FbaI         2001  TGATCA               T^GATCA
             5727  TGATCA              
HapII        1134  CCGG                 C^CGG
             3503  CCGG                
HgaI          734  GACGC                GACGC(5/10)
              721  GCGTC               
HincII       3948  GTTGAC               GT[CT]^[GA]AC
             5446  GTTGAC              
HindII       3948  GTTGAC               GT[CT]^[GA]AC
             5446  GTTGAC              
HindIII      1424  AAGCTT               A^AGCTT
             1563  AAGCTT              
HpaII        1134  CCGG                 C^CGG
             3503  CCGG                
MspI         1134  CCGG                 C^CGG
             3503  CCGG                
NcoI         4492  CCATGG               C^CATGG
             6144  CCATGG              
NdeI           86  CATATG               CA^TATG
             5784  CATATG              
NsiI           93  ATGCAT               ATGCA^T
             3214  ATGCAT              
PflFI        1228  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2473  GACTGAGTC           
PflMI        4492  CCATGGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             6731  CCACCTCATGG         
PshBI         839  ATTAAT               AT^TAAT
             4678  ATTAAT              
RcaI         3228  TCATGA               T^CATGA
             5267  TCATGA              
SacI         2540  GAGCTC               GAGCT^C
             2887  GAGCTC              
SapI         1547  GAAGAGC              GCTCTTC(1/4)
             2099  GAAGAGC             
SpeI         6429  ACTAGT               A^CTAGT
             6441  ACTAGT              
Tth111I      1228  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2473  GACTGAGTC           
Van91I       4492  CCATGGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             6731  CCACCTCATGG         
XcmI         1242  CCATAGTCAAAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1776  CCAGATTATATTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
Alw44I      AlwI        ApaLI       ApoI        Asp700I     
AspHI       AvaII       BanII       BbsI        BbvI        
BfaI        BglII       BmyI        BpuAI       BsaBI       
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    Bsp1407I    
BspLU11I    BsrGI       BsrI        BssKI       BstF5I      
BstNI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        EarI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HhaI        HinP1I      HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MamI        
MboI        MboII       MflI        MnlI        MseI        
MslI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspBI       SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       XmnI