##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII AclI AflII AscI AseI
AsnI AviII AvrII BbeI BbrPI
BfrI BlnI BsiWI BspDI BsrDI
Bst1107I BstZ17I ClaI CpoI EcoRI
EcoRV FseI FspI KasI KspI
MluI NarI NdeI NheI NotI
PacI PaeR7I PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfoI
SgrAI SnaBI SpeI Sse8387I SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AcsI 3324 GAATTT [GA]^AATT[CT]
AgeI 499 ACCGGT A^CCGGT
AhdI 5904 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2498 GTGCAC G^TGCAC
Aor51HI 1094 AGCGCT AGC^GCT
ApaLI 2498 GTGCAC G^TGCAC
ApoI 3324 GAATTT [GA]^AATT[CT]
AspEI 5904 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 5020 GTATCC GTATCC(6/5)
BclI 395 TGATCA T^GATCA
BglII 4622 AGATCT A^GATCT
BsaAI 18 TACGTG [CT]AC^GT[GA]
BsaBI 2723 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 398 TCATGA T^CATGA
BssHII 221 GCGCGC G^CGCGC
BstBI 355 TTCGAA TT^CGAA
DraI 411 TTTAAA TTT^AAA
DrdI 5280 GACCGCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 5130 CGGCCG C^GGCCG
Eam1105I 5904 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 5130 CGGCCG C^GGCCG
Eco47III 1094 AGCGCT AGC^GCT
Eco52I 5130 CGGCCG C^GGCCG
EcoT22I 3585 ATGCAT ATGCA^T
FbaI 395 TGATCA T^GATCA
HpaI 1305 GTTAAC GTT^AAC
MamI 2723 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3828 CAATTG C^AATTG
MunI 3828 CAATTG C^AATTG
NsiI 3585 ATGCAT ATGCA^T
NspV 355 TTCGAA TT^CGAA
PinAI 499 ACCGGT A^CCGGT
RcaI 398 TCATGA T^CATGA
ScaI 3104 AGTACT AGT^ACT
SfuI 355 TTCGAA TT^CGAA
SmaI 3643 CCCGGG CCC^GGG
SspI 616 AATATT AAT^ATT
XmaCI 3643 CCCGGG C^CCGGG
XmaI 3643 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Acc65I 3232 GGTACC G^GTACC
3467 GGTACC
AccI 1572 GTAGAC GT^[AC][GT]AC
1890 GTCTAC
AccIII 763 TCCGGA T^CCGGA
973 TCCGGA
Asp700I 140 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3728 GAACTGCTTC
Asp718I 3232 GGTACC G^GTACC
3467 GGTACC
BamHI 766 GGATCC G^GATCC
5107 GGATCC
BcgI 544 CGAGCCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4064 CGAGGAGGCTGC
BseAI 763 TCCGGA T^CCGGA
973 TCCGGA
BsiEI 4466 CGACCG CG[GA][CT]^CG
5130 CGGCCG
BsmBI 2873 CGTCTC CGTCTC(1/5)
2991 GAGACG
BsmI 7063 GAATGC GAATGC(1/-1)
3655 GCATTC
BspEI 763 TCCGGA T^CCGGA
973 TCCGGA
BstEII 2121 GGTGACC G^GT[ACGT]ACC
6690 GGTCACC
BstPI 2121 GGTGACC G^GT[ACGT]ACC
6690 GGTCACC
EcoO65I 2121 GGTGACC G^GT[ACGT]ACC
6690 GGTCACC
KpnI 3232 GGTACC GGTAC^C
3467 GGTACC
MroI 763 TCCGGA T^CCGGA
973 TCCGGA
NruI 96 TCGCGA TCG^CGA
238 TCGCGA
SapI 6396 GAAGAGC GCTCTTC(1/4)
5060 GCTCTTC
SphI 3710 GCATGC GCATG^C
3895 GCATGC
XmnI 140 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3728 GAACTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 146 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AspI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspLU11I BspMI BsrBI
BsrFI BsrGI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII