##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AgeI AhdI ApaI AscI
AspEI AviII AvrII BbeI BbrPI
BbvCI BcgI BfrI BlnI BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BsrBI BssHII Bst1107I
BstZ17I ClaI CpoI DraII DraIII
EagI Eam1105I EclXI Eco52I EcoO109I
EcoRI EcoRV FseI FspI HgaI
Hin1I HpaI KasI KspI MamI
MluI MroI NarI NotI NruI
PacI PflMI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PvuI RsrII
SacI SacII SalI SbfI SfiI
SfoI SgrAI SmaI Sse8387I Van91I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 411 GGTACC G^GTACC
AccII 2952 CGCG CG^CG
Alw44I 5222 GTGCAC G^TGCAC
Aor51HI 3796 AGCGCT AGC^GCT
ApaLI 5222 GTGCAC G^TGCAC
AseI 4238 ATTAAT AT^TAAT
AsnI 4238 ATTAAT AT^TAAT
Asp718I 411 GGTACC G^GTACC
BamHI 5766 GGATCC G^GATCC
BsaWI 2437 ACCGGA [AT]^CCGG[AT]
BssSI 2419 CTCGTG CACGAG(-5/-1)
BstBI 547 TTCGAA TT^CGAA
BstEII 5539 GGTGACC G^GT[ACGT]ACC
BstPI 5539 GGTGACC G^GT[ACGT]ACC
BstUI 2952 CGCG CG^CG
DrdI 5828 GACTTTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3796 AGCGCT AGC^GCT
EcoO65I 5539 GGTGACC G^GT[ACGT]ACC
KpnI 411 GGTACC GGTAC^C
MvnI 2952 CGCG CG^CG
NcoI 45 CCATGG C^CATGG
NspV 547 TTCGAA TT^CGAA
PshAI 895 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4238 ATTAAT AT^TAAT
SfuI 547 TTCGAA TT^CGAA
SnaBI 2145 TACGTA TAC^GTA
SwaI 4342 ATTTAAAT ATTT^AAAT
XbaI 2888 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AspHI 5151 GTGCTC G[AT]GC[AT]^C
5222 GTGCAC
AspI 581 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4016 GACCGAGTC
BanII 2231 GAGCCC G[GA]GC[CT]^C
2681 GAGCCC
BclI 2206 TGATCA T^GATCA
2666 TGATCA
BlpI 2520 GCTGAGC GC^T[ACGT]AGC
3245 GCTGAGC
Bpu1102I 2520 GCTGAGC GC^T[ACGT]AGC
3245 GCTGAGC
BsaAI 1383 CACGTA [CT]AC^GT[GA]
2145 TACGTA
BsaI 2979 GAGACC GGTCTC(1/5)
4014 GAGACC
BsiHKAI 5151 GTGCTC G[AT]GC[AT]^C
5222 GTGCAC
BspHI 1363 TCATGA T^CATGA
4080 TCATGA
BspMI 716 ACCTGC ACCTGC(4/8)
2175 ACCTGC
BsrFI 1792 GCCGGC [GA]^CCGG[CT]
2083 GCCGGC
CelII 2520 GCTGAGC GC^T[ACGT]AGC
3245 GCTGAGC
Cfr10I 1792 GCCGGC [GA]^CCGG[CT]
2083 GCCGGC
EcoNI 3113 CCTACCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5058 CCTGTTTAAGG
FauI 2606 CCCGC CCCGC(4/6)
2505 GCGGG
FbaI 2206 TGATCA T^GATCA
2666 TGATCA
HaeII 1965 AGCGCC [GA]GCGC^[CT]
3796 AGCGCT
HincII 3461 GTCAAC GT[CT]^[GA]AC
4208 GTTGAC
HindII 3461 GTCAAC GT[CT]^[GA]AC
4208 GTTGAC
MfeI 3970 CAATTG C^AATTG
4442 CAATTG
MunI 3970 CAATTG C^AATTG
4442 CAATTG
NaeI 1792 GCCGGC GCC^GGC
2083 GCCGGC
NdeI 1371 CATATG CA^TATG
1569 CATATG
NgoMIV 1792 GCCGGC G^CCGGC
2083 GCCGGC
NheI 692 GCTAGC G^CTAGC
2705 GCTAGC
PaeR7I 2185 CTCGAG C^TCGAG
3466 CTCGAG
PflFI 581 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4016 GACCGAGTC
RcaI 1363 TCATGA T^CATGA
4080 TCATGA
SapI 2228 GAAGAGC GCTCTTC(1/4)
2529 GAAGAGC
ScaI 2777 AGTACT AGT^ACT
4454 AGTACT
SexAI 755 ACCTGGT A^CC[AT]GGT
4151 ACCAGGT
SpeI 252 ACTAGT A^CTAGT
395 ACTAGT
SphI 3508 GCATGC GCATG^C
5053 GCATGC
StuI 216 AGGCCT AGG^CCT
2869 AGGCCT
Tth111I 581 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4016 GACCGAGTC
XcmI 814 CCATCCCTTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5714 CCAGAAACAGCTTGG
XhoI 2185 CTCGAG C^TCGAG
3466 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI Asp700I
AvaI AvaII BalI BanI BbsI
BbvI BciVI BcnI BfaI BglI
BglII BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspLU11I BsrDI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DsaI EaeI EarI
Eco81I EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI